Decreased levels of serum glutathione peroxidase 3 are associated with papillary serous ovarian cancer and disease progression

<p>Abstract</p> <p>Background</p> <p>Glutathione peroxidase 3 (GPX3) is a selenocysteine-containing antioxidant enzyme that reacts with hydrogen peroxide and soluble fatty acid hydroperoxides, thereby helping to maintain redox balance within cells. Serum levels of GPX3 have been found to be reduced in various cancers including prostrate, thyroid, colorectal, breast and gastric cancers. Intriguingly, GPX3 has been reported to be upregulated in clear cell ovarian cancer tissues and thus may have implications in chemotherapeutic resistance. Since clear cell and serous subtypes of ovarian cancer represent two distinct disease entities, the aim of this study was to determine GPX3 levels in serous ovarian cancer patients and establish its potential as a biomarker for detection and/or surveillance of papillary serous ovarian cancer, the most frequent form of ovarian tumors in women.</p> <p>Patients and Methods</p> <p>Serum was obtained from 66 patients (median age: 62 years, range: 22-89) prior to surgery and 65 controls with a comparable age-range (median age: 53 years, range: 25-83). ELISA was used to determine the levels of serum GPX3. The Mann Whitney <it>U </it>test was performed to determine statistical significance between the levels of serum GPX3 in patients and controls.</p> <p>Results</p> <p>Serum levels of GPX3 were found to be significantly lower in patients than controls (p = 1 × 10^-2). Furthermore, this was found to be dependent on the stage of disease. While levels in early stage (I/II) patients showed no significant difference when compared to controls, there was a significant reduction in late stage (III/IV, p = 9 × 10^-4) and recurrent (p = 1 × 10^-2) patients. There was a statistically significant reduction in levels of GPX3 between early and late stage (p = 5 × 10^-4) as well as early and recurrent (p = 1 × 10^-2) patients. Comparison of women and controls stratified to include only women at or above 50 years of age shows that the same trends were maintained and the differences became more statistically significant.</p> <p>Conclusions</p> <p>Serum GPX3 levels are decreased in women with papillary serous ovarian cancer in a stage-dependent manner and also decreased in women with disease recurrence. Whether this decrease represents a general feature in response to the disease or a link to the progression of the cancer is unknown. Understanding this relationship may have clinical and therapeutic consequences for women with papillary serous adenocarcinoma.</p

Innovación, desarrollo tecnológico y gestión : una construcción desde la investigación

Libro que compila investigaciones de carácter aplicadas y descriptivas en materia de innovación y desarrollo tecnológico, gestión organizacional y empresarial orientada a productividad, rentabilidad, competitividad y sostenibilidadBook that compiles researches of applied and descriptive character in the matter of innovation and technological development, organizational and business management oriented to productivity, profitability, competitiveness and sustainabilityCapítulo 1. Material compuesto para la construcción a partir de la celulosa del papel y cartón reciclado / Carlos Arturo Tamayo S; Nicolás Montero Camacho; Fredy Antonio Herrera -- Capítulo 2. Tecnologías de conservación para base de sopa de frijol rojo (phaseolus vulgaris) y vegetales / Yaceris Castro Escorcia; Teresa Altamar Pérez; Enedys Florez Cortés; Ángela Ortiz Ruiz. Capítulo 3. Utilización de harina compuesta de frijol caupi (vigna unguiculata) en masas para alimentos congelados / Marcela Villalba Cadavid; Matilde Rodríguez Muñoz; Beatriz Fernández; Juan Mendoza Combatt -- Capítulo 4. Elaboración de biorrecubrimiento comestible para carne de hamburguesa como alternativa de conservación y condimento natural / Camila Andrea Ubaque Beltrán. CAPÍTULO 5. Identificación de alternativas de industrialización de productos y subproductos agroindustriales en nuevos materiales bio poliméricos / Luz Henao Díaz; Deya Pérez Zúñiga; Herold Arango Gómez. Capítulo 6. SCADA inalámbrico para monitoreo de sistemas de energía solar / Nelson Giovanni Agudelo Cristancho; Juan Carlos Amezquita Tovar; Ángela María Montoya Castro. -- Capítulo 7. Evaluación de la calidad del agua para consumo humano del corregimiento de Jaraquiel, Montería, Córdoba / Carlos Burgos Galeano; Álvaro Aleán Vásquez; Paula Estrada Palencia -- Capítulo 8. Optimización del sistema de abastecimiento de agua en la comunidad de Jaraquel, Montería Colombia / Carlos Burgos Galeano; Pedro Ramos Tejada; Paula Estrada Palencia; Jhon Sánchez Correa. -- Capítulo 9. Ejecución y sostenibilidad de proyectos productivos en la microrregión cafetera del municipio de Ciénaga, Magdalena / Sugey Issa Fontalvo; Eduardo Robles Panetta; Freddy González Castillo. -- Capítulo 10. Aplicación del método cualitativo por puntos para determinar aspirante favorito a cargos directivos en IES / Zamir Martelo Ballesteros; Raúl Martelo Gómez; Luis Tovar Garrido; Natividad Villabona Gómez; David Franco Borré. -- Capítulo 11 Responsabilidad social en comunidades indígenas orientada al diseño de automatización de válvulas del gasoducto Riohacha-Maicao / Gelvis Melo Freile; César Rivera Romero; Jesús García Guiliany. -- Capítulo 12 Fortalecimiento de la gestión económica de las Mipymes a través de la consultoría / Gloria Amparo Acosta Romero; Mónica Andrade Ríos; Karen Roxana Sánchez. -- Capítulo 13. El compromiso como valor en la responsabilidad social universitaria / Maura Quintero Gutiérrez, Dubys Villarreal Torres; Jesús García Guiliany; Annherys Paz Marcano; Marieth Orcasitas Peñaloza. -- Capítulo 14. Educación financiera como alternativa de desarrollo económico y social para el distrito de Riohacha / Henitzo Martínez Pinedo; Darcy Luz Mendoza; Martha Jaramillo Acosta; Edwin Salas Solano. -- Capítulo 15. Tecnologías de información y comunicación en proceso contable y financiero en pymes del sector turístico / Martha Josefina Castrillón Rois; Edilberto Rafael Santos Moreno; Lorena Esther Gómez Bermúdez; Génesis Barros González. -- Capítulo 16. Aplicación de brainstorming y problem trees para determinar factores que inciden en enseñanza del inglés / Jesús Llerena; Raúl J. Martelo; Jhon Cuesta; Javier Pinedo; David Franco.-- Capítulo 17. Incidencia del marketing en las microempresas del sector comercio en Rionegro Antioquia: conceptualización / Santiago Álzate Carmona; María Yamile Mazo Gil; Leidy García Jaramillo. -- Capítulo 18. Turismo en el Cabo de la Vela: un acercamiento entre los imaginarios turísticos de los visitantes y la creencia de Jepirra, territorio sagrado / María Laura Aponte Aarón; Esmerlis Camargo Torres. -- Capítulo 19. Caracterización de la actividad turística en buenaventura y su integración con las comunidades locales / Víctor Cándelo Aragón; Henry Orobio García; Luis Montaño Aguilar. -- Capítulo 20. Plataforma de comercialización electrónica de un centro de abastos / Karen Ávila Suarez; Mauro Reyes Ortiz. -- Capítulo 21. El teletrabajo en la gestión administrativa / Estefanía Sandoval Cruz; René Alexander Guerrero Vergel. -- Capítulo 22. Gestión del conocimiento y alianzas estratégicas en los procesos de innovación tecnológica / Elder Rivero Gutiérrez; Fátima Bolaño Mendoza. -- Capítulo 23. Competitividad e innovación en el aprendiz SENA: perspectivas de formación / Elizabeth Tuberquia Vanegas; René Alexander Guerrero Vergel. -- Capítulo 24. Bomba de riego por goteo solar, una alternativa para aumentar la eficiencia energética en las unidades acuícolas / Sergio Gabriel Brito Brito; Daldo Ricardo Araujo Vidal; Nicolás Annicharico Jiménez. -- Capítulo 25. Herramienta digital de consultas contables y tributarias para unidades productivas creadas en el fondo emprender / Elkin Fuentes Jiménez; Alda Pérez Campuzano; Marieth Orcasitas Peñaloza; Olga Elena Guerra ArmentaPrimera ediciónna347 página

Alterazioni ribosomali in eucarioti superiori: ricerca e costruzione di mutanti

Dottorato di ricerca in biologia cellulare e molecolareConsiglio Nazionale delle Ricerche - Biblioteca Centrale - P.le Aldo Moro, 7, Rome; Biblioteca Nazionale Centrale - P.za Cavalleggeri, 1, Florence / CNR - Consiglio Nazionale delle RichercheSIGLEITItal

Mutations in Diaphyseal Medullary Stenosis- Malignant Fibrous Histiocytoma Related Gene MTAP Affects Expression of Splice Variants SV2 and SV5

Diaphyseal Medullary Stenosis- Malignant Fibrous Histiocytoma (DMS-MFH) is a rare autosomal dominant bone syndrome. Thirty-five percent of patients diagnosed with DMS-MFH are at risk of developing a bone sarcoma, malignant fibrous histiocytoma (MFH). Symptoms of this bone disease include bone infarctions, bone pain, leg weakness, and the development of early-onset cataracts. Recently, studies in our laboratory have shown that either of two inherited mutations, IVS8 (-2) A&gt;G or Ex9 (+72) A&gt;G in the MTAP gene locus, results in the development of DMS-MFH. These mutations specifically target the methylthioadenosine phosphorylase (MTAP) gene, located on chromosome 9p21.3. Our laboratory has now shown that the MTAP gene encodes six alternative splicing isoforms

Clinical Characterization Of Myopathy In A Rare Autosomal Disease: Hereditary Bone Dysplasia/Osteosarcoma And Limb Girdle Myopathy In A Unique Family

Autosomal‐dominant myopathic disorder associated with diaphyseal medullary stenosis with malignant fibrous his?ocytoma (DMS‐MFH) is characterized by myopathy, bone fragility, and osteosarcoma. DMS‐MFH was recently associated with muta?ons in the methylthioadenosine phosphorylase gene (MTAP). MTAP is a ubiquitously expressed enzyme crucial for polyamine biosynthesis. Two disease‐causing muta?ons have been iden?fied in MTAP: c.813‐2A&gt;G and c.885A&gt;G, both of which result in dysregulated alterna?ve splicing of MTAP isoforms. Here, we report on myopathy in two cousins with the c.813‐2A&gt;G muta?on. Both developed a progressive limb‐girdle type myopathy at age 30 years. To our knowledge, we are the first group to characterize the myopathy associated with DMS‐MFH, discovering varied muscle fiber size, degenera?on, and increased centralized nuclei. In this report, expression levels of transac?ve response DNA‐binding protein (TDP)‐43, light chain (LC)3‐I/II, and p62/sequestome 1 (SQSTM1) in the muscle fibers were increased, sugges?ng a possible dysregula?on of autophagy. Elucida?on of the pathologic mechanism(s) in DMS‐MFH offers the poten?al to uncover key molecular signaling pathways and the promise of novel future treatments