Structure of cation channels, revealed by single particle electron microscopy

AbstractA large barrier in the way to obtaining high-resolution structures of eukaryotic ion channels remains the expression and purification of sufficient amounts of channel protein to carry out crystallization trials. In the absence of crystals, the main available source of structural information has been electron microscopy (EM), which is well suited to the visualization of isolated macromolecular complexes and their conformational changes. The recently published EM structures outline native conformations of eukaryotic cation channels that until now have eluded crystallization. According to these results, homo-tetrameric K+ channels have a distinct two-layer architecture with connectors conjoining the two layers, while the pseudo-tetrameric Ca2+ or Na+ channels are more globular and have flexible surface loops, which makes the identification of subunits complicated. Subunits can be identified using atomic structure docking into the EM maps, labeling, or deletion studies

Justification of the Recipe Composition of Biscuit Using Functional Additives

Increased production of processed foods, rapid urbanization and changing lifestyles have led to a shift in dietary patterns. Nowadays, people consume more foods high in calories, fat, free sugars, and salt/sodium, and many do not consume enough fruits, vegetables, and other types of fiber such as whole grains. The article describes the principles of healthy nutrition, which are justified by the World Health Organization (WHO). The recipe composition of the biscuit using a mixture of potato and corn starches, oat bran replace part of the sugar on stevioside with the addition of functional additives in the Arctic region, namely black currant juice, grown in Lovozero. It is known that black currant contains vitamins, organic acids, sugars (fructose and glucose), flavonoids, pectin, minerals and adding it to the formulation can increase nutritional and functional value. The article shows the results of the study of biscuit quality using functional additives of the Arctic region. The composition of the biscuit: the ratio of the added vegetable raw materials is scientifically proved. A point scale of quality of functional biscuit products with the use of additives from the raw materials of the Arctic region was developed. The composition of the finished product was investigated

DIGITAL TECHNOLOGIES IN INNOVATIVE AND STRUCTURAL TRANSFORMATION OF UKRAINE'S ECONOMY

The article substantiates the role of artificial intelligence in procuring the management of innovation and structural transformation of the economy. The analysis of structural shifts in gross value added in the economy of Ukraine during 2002–2019 is carried out. Solow’s residuals are calculated for the primary, secondary and tertiary sectors. The expediency of managing the innovative and structural transformation of Ukraine's economy on the basis of changes in the level of Solow's residual in that sector and in the period of time determined by big data analysis by artificial intelligence is proved. A VAR model was built in the EViews environment for Solow’s residuals in three sectors of the Ukrainian economy. Based on variance decomposition and IRF, it is established that increasing the share of intermediate consumption by the secondary sector of education, ICT, professional, scientific and technical services will increase Solow's residual in the secondary sector in two years, in the tertiary sector in three years and in the primary sector in four years, which will affect the increase in output in Ukraine during these three years.The article substantiates the role of artificial intelligence in procuring the management of innovation and structural transformation of the economy. The analysis of structural shifts in gross value added in the economy of Ukraine during 2002–2019 is carried out. Solow’s residuals are calculated for the primary, secondary and tertiary sectors. The expediency of managing the innovative and structural transformation of Ukraine's economy on the basis of changes in the level of Solow's residual in that sector and in the period of time determined by big data analysis by artificial intelligence is proved. A VAR model was built in the EViews environment for Solow’s residuals in three sectors of the Ukrainian economy. Based on variance decomposition and IRF, it is established that increasing the share of intermediate consumption by the secondary sector of education, ICT, professional, scientific and technical services will increase Solow's residual in the secondary sector in two years, in the tertiary sector in three years and in the primary sector in four years, which will affect the increase in output in Ukraine during these three years

Elaborating principles of idiom-formation in the avant-garde literary discourse: a case study of Russian Cubo-Futurism

The paper sets out to explore the avant-garde literary discourse from the point of view of its idiomatic constituent. In particular, it studies avant-garde manifestoes written by the representatives of Cubo- Futurism, in which radical aesthetic renewal and the need for the reformation in the sphere of literature are proclaimed. As very expressive and complex rhetorical performances, manifestoes contain various kinds of idiomatic phrases that can be considered both vivid representations of a particular (avant-garde) approach to the language and prescriptions for certain ways of the language use. Special attention is paid to the elaboration of the methodology aimed to identify avant-garde idioms proper in the avant-garde literary discourse. The results obtained show that the manifestoes under consideration are, in essence, the first “trials” in the field of (poetic) language reformation and language experimentation and the sources of the idiomatics (idioms / idiomatic expressions) that can be termed “experimental”

Characterization of Virulence Factors of Staphylococcus aureus: Novel Function of Known Virulence Factors That Are Implicated in Activation of Airway Epithelial Proinflammatory Response

Airway epithelial cells play a major role in initiating inflammation in response to bacterial pathogens. S. aureus is an important pathogen associated with activation of diverse types of infection characterized by inflammation dominated by polymorphonuclear leukocytes. This bacterium frequently causes lung infection, which is attributed to virulence factors. Many of virulence determinants associated with S. aureus-mediated lung infection have been known for several years. In this paper, we discuss recent advances in our understanding of known virulence factors implicated in pneumonia. We anticipate that better understanding of novel functions of known virulence factors could open the way to regulate inflammatory reactions of the epithelium and to develop effective strategies to treat S. aureus-induced airway diseases

Role of Uropathogenic Escherichia coli Virulence Factors in Development of Urinary Tract Infection and Kidney Damage

Uropathogenic Escherichia coli (UPEC) is a causative agent in the vast majority of urinary tract infections (UTIs), including cystitis and pyelonephritis, and infectious complications, which may result in acute renal failure in healthy individuals as well as in renal transplant patients. UPEC expresses a multitude of virulence factors to break the inertia of the mucosal barrier. In response to the breach by UPEC into the normally sterile urinary tract, host inflammatory responses are triggered leading to cytokine production, neutrophil influx, and the exfoliation of infected bladder epithelial cells. Several signaling pathways activated during UPEC infection, including the pathways known to activate the innate immune response, interact with calcium-dependent signaling pathways. Some UPEC isolates, however, might possess strategies to delay or suppress the activation of components of the innate host response in the urinary tract. Studies published in the recent past provide new information regarding how virulence factors of uropathogenic E. coli are involved in activation of the innate host response. Despite numerous host defense mechanisms, UPEC can persist within the urinary tract and may serve as a reservoir for recurrent infections and serious complications. Presentation of the molecular details of these events is essential for development of successful strategies for prevention of human UTIs and urological complications associated with UTIs

SOME ASPECTS OF THE PSYCHOLOGICAL AND PEDAGOGICAL SUPPORT FOR THE ADAPTATION OF FUTURE TEACHERS

The article is devoted to the study of some aspects of psychological and pedagogical support for the adaptation of future teachers. Objective: to determine the impact of the proposed strategies of psychological and pedagogical support on the ability of students to adapt. The “Adaptability” (Multilevel Personality Questionnaire) questionnaire by A. Maklakov and S. Chermianin was used in the study to diagnose students of the 1st-6th years of bachelor’s and master’s degree programs of higher education at the faculty of arts of a pedagogical higher education establishment. The results of the introduction of strategies of psycho-pedagogical support showed that students grow their ability to regulate their interaction with the environment, that there is an increase in social contacts, social activity and self-esteem. It has been proven that the introduction of the proposed strategies of pedagogical support at the faculty of the arts of a pedagogical higher education establishment has a direct impact on the ability of students to adapt.

Quantum-chemical study of electronically excited states of protolytic forms of vanillic acid

The paper describes an analysis of possible ways of deactivation of electronically excited states of 4-hydroxy- 3-methoxy-benzoic acid (vanillic acid) and its protolytic forms with the use of quantum-chemical methods INDO/S (intermediate neglect of differential overlap with a spectroscopic parameterization) and MEP (molecular electrostatic potential). The ratio of radiative and non-radiative deactivation channels of the electronic excitation energy is established. The rate constants of photophysical processes (internal and intercombination conversions) occurring after the absorption of light in these forms are evaluated. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only