Physiological Approach to Filament Cells. Ⅰ. Studies On the Enzymic Aspects and Surface Structure of Filament Cells Induced by Penicillin

The abnormal filament formation of Sal. typhi was observed on penicillin containing nutrient agar in 1u/ml. Characters of the filament cells were compared with normally harvested cells in the view points of oxidative activities to some substrates, acid agglutinating property, glucosamine content and serological properties. No difference was observed about the oxidative activities, the glucosamine content and the serological properties. With respect to the acid agglutinating property, as comparsed with the normal cells, the filament cells exhibited a property to agglutinate in broader PH range

Physiological Approaceh to Filament Cells. 2. Studies On the Enzymic Aspects and Nucleic Acids Components of Filament Cells Induced By Mitomycin C

1. The abnormally elongated filament cell formafion was observed on Mitomycin C containing agar in 1 gamma per ml. In the filment cells, by cell wall staining method, no septum formation was observed notwithstanding cell wall structure was retained. By a method of chromatinic body staining, regularly arranged abundant chromatinic bodies were observed. 2. By a method of manometric technique, the filament cells exhibited low oxidation activities to the substrates on TCA cycle. 3. With respect to acid agglutination test, the filament cells were agglutinated at the different range as compared with normal cells. 4. In the course of the filament formation, RNA synthesis proceeded without inhibition but DNA synthesis was slightly inhibited

Analytical Studies on the Column Chromatographic Determination of DNA Base Composition with Ion Exchange Resins

1. Many type of methods has been designed for determination of nucleic acid base composition. In this paper the a curacy of the results obtained using ion exchgers were tested about calf thymus DNA. The column chromatographic methods yield satisfactory results. 2. In the course of the operation, nutralization of acidic solution of nucleic acid bases must be carried out carefully to avoid precipitation of purine bases