ICAMs support B cell interactions with T follicular helper cells and promote clonal selection.

The germinal center (GC) reaction begins with a diverse and expanded group of B cell clones bearing a wide range of antibody affinities. During GC colonization, B cells engage in long-lasting interactions with T follicular helper (Tfh) cells, a process that depends on antigen uptake and antigen presentation to the Tfh cells. How long-lasting T-B interactions and B cell clonal expansion are regulated by antigen presentation remains unclear. Here, we use in vivo B cell competition models and intravital imaging to examine the adhesive mechanisms governing B cell selection for GC colonization. We find that intercellular adhesion molecule 1 (ICAM-1) and ICAM-2 on B cells are essential for long-lasting cognate Tfh-B cell interactions and efficient selection of low-affinity B cell clones for proliferative clonal expansion. Thus, B cell ICAMs promote efficient antibody immune response by enhancement of T cell help to cognate B cells

Enhanced production yields of rVSV-SARS-CoV-2 vaccine using Fibra-Cel® macrocarriers

The COVID-19 pandemic has led to high global demand for vaccines to safeguard public health. To that end, our institute has developed a recombinant viral vector vaccine utilizing a modified vesicular stomatitis virus (VSV) construct, wherein the G protein of VSV is replaced with the spike protein of SARS-CoV-2 (rVSV-ΔG-spike). Previous studies have demonstrated the production of a VSV-based vaccine in Vero cells adsorbed on Cytodex 1 microcarriers or in suspension. However, the titers were limited by both the carrier surface area and shear forces. Here, we describe the development of a bioprocess for rVSV-ΔG-spike production in serum-free Vero cells using porous Fibra-Cel® macrocarriers in fixed-bed BioBLU®320 5p bioreactors, leading to high-end titers. We identified core factors that significantly improved virus production, such as the kinetics of virus production, the use of macrospargers for oxygen supply, and medium replenishment. Implementing these parameters, among others, in a series of GMP production processes improved the titer yields by at least two orders of magnitude (2e9 PFU/mL) over previously reported values. The developed process was highly effective, repeatable, and robust, creating potent and genetically stable vaccine viruses and introducing new opportunities for application in other viral vaccine platforms

Russia’s invasion of Ukraine increased the synchronisation of global commodity prices

The Russian Federation’s efforts to expand its regional political influence culminated in launching a full-scale war of aggression on Ukraine on 24 February 2022. As both countries are large exporters of commodities crucial for global food and energy security, the resulting abrupt supply chains disruptions created substantial uncertainty in commodity markets worldwide. This study quantifies to what extent this major shock induced global commodity prices to move more synchronously by gauging their time-varying comovement. Using the concordance index, it analyses the development of 15 key global commodity price indices from January 2010 to July 2022. We find that the supply chains disruptions increased synchronisation of grain, energy and fertiliser prices at the global level in direction and magnitude. Moreover, they resulted in contagion across numerous food and non-food markets, creating a global covariate shock to food and energy security. Notably, the increased synchronisation at broad scale restricts the ability of consumers to mitigate the adverse effects of food and energy price inflation by resorting to inexpensive alternatives. Hence, policymakers must improve the resilience of global food supply chains sustainably such that adverse effects of attaining the Sustainable Development Goals in crises can be minimised

Naturalistic Generative Narratives Reveal Effects of Social Characteristics on Decision-Making

The social characteristics of others can powerfully influence our decisions. They can also be broadly impacted by the social context in which these choices are made, making the effects of these characteristics on decision-making especially challenging to understand. Here, we developed a Generative Narrative Approach that provided participants with naturalistic scenarios that richly varied in social context and theme but that also systematically varied the characteristics of the social agents involved. After validating this approach using feeling thermometer measures, we find that the emotional states of others had the strongest and most consistent effect on the participants’ choices. More notably, whereas most characteristics had independent effects on decision-making, social features such as the inferred socioeconomic status of others significantly influenced the effect that race had on the participant’s judgments. Moreover, the social context of the agent’s interactions with other agents had a significant additive effect, especially when the emotional states of the agents in the scenarios contrasted. The influence of these characteristics on the participants’ choices was also markedly affected by their demographics, especially when contrasting with that of the agents involved, and were often driven by the participants' reported political views. Together, these findings reveal how the mixture of social characteristics, context, and personal views influence decision-making and highlight the use of naturalistic generative narrative approaches in studying human behavior

Russia’s invasion of Ukraine increased the synchronisation of global commodity prices

The Russian Federation’s efforts to expand its regional political influence culminated in launching a full-scale war of aggression on Ukraine on 24 February 2022. As both countries are large exporters of commodities crucial for global food and energy security, the resulting abrupt supply chains disruptions created substantial uncertainty in commodity markets worldwide. This study quantifies to what extent this major shock induced global commodity prices to move more synchronously by gauging their time-varying comovement. Using the concordance index, it analyses the development of 15 key global commodity price indices from January 2010 to July 2022. We find that the supply chains disruptions increased synchronisation of grain, energy and fertiliser prices at the global level in direction and magnitude. Moreover, they resulted in contagion across numerous food and non-food markets, creating a global covariate shock to food and energy security. Notably, the increased synchronisation at broad scale restricts the ability of consumers to mitigate the adverse effects of food and energy price inflation by resorting to inexpensive alternatives. Hence, policymakers must improve the resilience of global food supply chains sustainably such that adverse effects of attaining the Sustainable Development Goals in crises can be minimised

Improving Diagnostic Accuracy Using EHR in Emergency Departments: A Simulation-Based Study

It is widely believed that electronic health records (EHR) improve medical decision making by enabling medical staff to access medical information stored in the system. It remains unclear, however, whether EHR indeed fulfills this claim under the severe time constraints of Emergency Departments (EDs). We assessed whether accessing EHR in an ED actually improves decision-making by clinicians. An authentic simulated ED environment was created at the Israel Center for Medical Simulation (MSR). Four different actors were trained to simulate four specific complaints and behavior. Each physician treated half of the cases (randomly) with access to EHR, and their medical decisions were compared to those where the physicians had no access to EHR. Accessing the EHR led to an increase in the quality of the clinical decisions. The percentage of correct diagnoses was higher and physicians were more confident in their diagnoses

Brand names act like marketing placebos

This research illustrates the power of reputation, such as that embodied in brand names, demonstrating that names can enhance objective product efficacy. Study participants facing a glaring light were asked to read printed words as accurately and as quickly as they could, receiving compensation proportional to their performance. Those wearing sunglasses tagged Ray-Ban made fewer errors, yet read more quickly, than those wearing the identical pair of sunglasses when tagged Mango (a less prestigious brand). Similarly, ear-muffs blocked noise more effectively, and chamomile tea improved mental focus more, when otherwise identical target products carried more reputable names.