2 research outputs found
Molecular Identification And Population Dynamics Of The Major Malaria Vectors In A Rainforest Zone Of Nigeria
Adult female mosquito vectors were collected from three villages in a
typical rain forest area of Nigeria where no information exists on the
major malaria vectors associated with human malaria. Sampling was
carried out between January 2004 and January 2005 using pyrethrum and
Human landing catch (HLC) techniques. A total catch of 2010 mosquitoes
was recorded out of which 1800 were morphologically identified as
female Anopheline mosquitoes. Further identification of the Anopheline
species using the morphological keys revealed that 1399 (77.7%)
belonged to the Anopheles gambiae s.l. and 401 (22.3%) to Anopheles
funestus . A PCR based test on the Anopheles gambiae group identified
636 (45.5%) as Anopheles gambiae s.s and 763 (54.5%) as Anopheles
arabiensis respectively. The cocktail PCR-assayon the total Anopheles
funestus group showed 307 (76.6%), to be Anopheles funestus s.s and 94
(23.4%) to be Anopheles leesoni . The total number of Anopheles
gambiae collected across the 3 villages was significantly higher
(P<0.05) than the number of Anopheles funestus caught within the
same period. However, there was a seasonal difference in the population
of Anopheline species collected in which the wet season collections
constitute 45.4% An.gambiae s.l and 17.7% An.funestus while the dry
season population constitutes 32.3% An.gambiae s.l and 4.5%
An.funestus. The dry seasoncollections were predominantly An.
arabiensis producing 23.9% of the total catch in.The overall number of
Anopheles mosquitoes collected in the wet season was significantly
higher than that of the dry season (P<0.01). Generally, low
sporozoite rates were recorded in all the communities and this may be
an indication that transmission in this area is less intense. This
study provides information on mosquito ecology, genetic and molecular
techniques for identification of species complexes which are important
strategies for planning malaria control programmes
Molecular identification and population dynamics of the major malaria vectors in a rainforest zone of Nigeria
Adult female mosquito vectors were collected from three villages in a typical rain forest area of Nigeria where no information exists on the major malaria vectors associated with human malaria. Sampling was carried out between January 2004 and January 2005 using pyrethrum and Human landing catch (HLC) techniques. A total catch of 2010 mosquitoes was recorded out of which 1800 were morphologically identified as female Anopheline mosquitoes. Further identification of the Anopheline species using the morphological keys revealed that 1399 (77.7%) belonged to the Anopheles gambiae s.l. and 401 (22.3%) to Anopheles funestus. A PCR based test on the Anopheles gambiae group identified 636 (45.5%) as Anopheles gambiae s.s and 763 (54.5%) as Anopheles arabiensis respectively. The cocktail PCR-assay on the total Anopheles funestus group showed 307 (76.6%), to be Anopheles funestus s.s and 94 (23.4%) to be Anopheles leesoni. The total number of Anopheles gambiae collected across the 3 villages was significantly higher (