Lactobacillus crispatus as the etiological agent in cytolytic vaginosis

Introduction: Lactobacillus spp. dominate the vaginal niche but can also be involved in other vaginal dysbiosis, such as cytolytic vaginosis (CV), which remains poorly studied. It is characterized by a cryptic symptomatology, that often confounds the clinic. Goals: The aim of this work was to search for the etiological agent of CV, by studying the vaginal microbiome and metabolomics of women afflicted with this disease and compare it with women with other clinical diagnostic. Methods: Twenty-one vaginal washes have been collected from women attending a gynaecology consultation of a private clinic. The samples were categorized according with clinical diagnosis at the time of sampling (CV, 11; vulvovaginal candidosis, 8; Healthy, 2). The distribution of bacterial species, and their prevalence was assessed by next-generation sequencing of the 16S V4 region. In addition, total lactate D-lactic acid and L-lactic acid was quantified in all washes by a commercial kit, as well as lactate dehydrogenase (LDH) activity. Results: L. crispatus was dominant (>70%) in all CV samples. Lactate was increased in CV in comparison with other cases. The presence of D-lactic acid isomer was associated with presence of L. crispatus. LDH activity was increased in vaginal washes that tested positive for the presence of L. crispatus, however no direct association was found with CV cases. Discussion/Conclusions: The microbiome of women afflicted with CV was dominated in all cases by L. crispatus, contrarily with the results obtained for women diagnosed with other clinical symptomatology. In addition, the finding that an increase in D-lactic acid is associated with CV patients can be related to the role of L. crispatus in CV. The determination of LDH activity did not correlate exclusively with CV cases. On the other hand, D-lactic acid and total lactate quantification could be used as a valuable biomarker to diagnose this cryptic vaginal infection.info:eu-repo/semantics/publishedVersio

The low-mass Initial Mass Function in the young cluster NGC 6611

NGC 6611 is the massive young cluster (2-3 Myr) that ionises the Eagle Nebula. We present very deep photometric observations of the central region of NGC 6611 obtained with the Hubble Space Telescope and the following filters: ACS/WFC F775W and F850LP and NIC2 F110W and F160W, loosely equivalent to ground-based IZJH filters. This survey reaches down to I ~ 26 mag. We construct the Initial Mass Function (IMF) from ~ 1.5 Msun well into the brown dwarf regime (down to ~ 0.02 Msun). We have detected 30-35 brown dwarf candidates in this sample. The low-mass IMF is combined with a higher-mass IMF constructed from the groundbased catalogue from Oliveira et al. (2005). We compare the final IMF with those of well studied star forming regions: we find that the IMF of NGC 6611 more closely resembles that of the low-mass star forming region in Taurus than that of the more massive Orion Nebula Cluster (ONC). We conclude that there seems to be no severe environmental effect in the IMF due to the proximity of the massive stars in NGC 6611.Comment: accepted for publication in MNRAS (main journal); 18 pages, 12 figures and 3 table

Star Formation in the Eagle Nebula and NGC 6611

We present IZJHKL' photometry of the core of the cluster NGC 6611 in the Eagle Nebula. This photometry is used to constrain the Initial Mass Function (IMF) and the circumstellar disk frequency of the young stellar objects. Optical spectroscopy of 258 objects is used to confirm membership and constrain contamination as well as individual reddening estimates. Our overall aim is to assess the influence of the ionizing radiation from the massive stars on the formation and evolution of young low-mass stars and their disks. The disk frequency determined from the JHKL' colour-colour diagram suggests that the ionizing radiation from the massive stars has little effect on disk evolution (Oliveira et al. 2005). The cluster IMF seems indistinguishable from those of quieter environments; however towards lower masses the tell-tale signs of an environmental influence are expected to become more noticeable, a question we are currently addressing with our recently acquired ultra-deep (ACS and NICMOS) HST images.Comment: in "Triggered Star Formation in a Turbulent ISM", IAU symposium, poster contribution; a full version of the poster can be found at http://www.astro.keele.ac.uk/~jacco/papers/IAUS237_Eagle_2006.pd

Cool stars in NGC 2547 and pre main sequence lithium depletion

We present the results of a spectroscopic survey of X-ray selected, low-mass candidate members of the young open cluster NGC 2547. Using a combination of photometry, spectroscopic indices and radial velocities we refine our candidate list and then use our spectroscopy to study the progression of lithium depletion in low-mass pre main sequence stars. We derive lithium abundances or upper limits for all our candidate members, which have effective temperatures in the range 5000>Teff>3200K, and compare these with predictions for lithium burning and depletion provided by a number of models and also with the lithium depletion seen in younger and older stars. We find that some models can reproduce the lithium abundance pattern of NGC 2547 if the cluster has an age of ~20-35Myr, which is also indicated by fits to low-mass isochrones in the Hertzsprung-Russell diagram. But the lack of significant further lithium depletion between NGC 2547 and older clusters argues for an age of at least 50Myr, more in keeping with the lack of lithium observed in even fainter NGC 2547 candidates. We show that reconciliation of these age estimates may require additions to the physics incorporated in current generations of pre main sequence models.Comment: Accepted for publication in MNRAS (better version of Fig.1 available at http://www.astro.keele.ac.uk/~rdj/