Biological sample donation and informed consent for neurobiobanking: Evidence from a community survey in Ghana and Nigeria

Copyright: \ua9 2022 Singh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Introduction Genomic research and neurobiobanking are expanding globally. Empirical evidence on the level of awareness and willingness to donate/share biological samples towards the expansion of neurobiobanking in sub-Saharan Africa is lacking. Aims To ascertain the awareness, perspectives and predictors regarding biological sample donation, sharing and informed consent preferences among community members in Ghana and Nigeria. Methods A questionnaire cross-sectional survey was conducted among randomly selected community members from seven communities in Ghana and Nigeria. Results Of the 1015 respondents with mean age 39.3 years (SD 19.5), about a third had heard of blood donation (37.2%, M: 42.4%, F: 32.0%, p = 0.001) and a quarter were aware of blood sample storage for research (24.5%; M: 29.7%, F: 19.4%, p = 0.151). Two out of ten were willing to donate brain after death (18.8%, M: 22.6%, F: 15.0%, p<0.001). Main reasons for unwillingness to donate brain were; to go back to God complete (46.6%) and lack of knowledge related to brain donation (32.7%). Only a third of the participants were aware of informed consent (31.7%; M: 35.9%, F: 27.5%, p<0.001). Predictors of positive attitude towards biobanking and informed consent were being married, tertiary level education, student status, and belonging to select ethnic groups. Conclusion There is a greater need for research attention in the area of brain banking and informed consent. Improved context-sensitive public education on neurobiobanking and informed consent, in line with the sociocultural diversities, is recommended within the African sub region

Novel functional insights into ischemic stroke biology provided by the first genome-wide association study of stroke in indigenous Africans

\ua9 The Author(s) 2024. Background: African ancestry populations have the highest burden of stroke worldwide, yet the genetic basis of stroke in these populations is obscure. The Stroke Investigative Research and Educational Network (SIREN) is a multicenter study involving 16 sites in West Africa. We conducted the first-ever genome-wide association study (GWAS) of stroke in indigenous Africans. Methods: Cases were consecutively recruited consenting adults (aged > 18 years) with neuroimaging-confirmed ischemic stroke. Stroke-free controls were ascertained using a locally validated Questionnaire for Verifying Stroke-Free Status. DNA genotyping with the H3Africa array was performed, and following initial quality control, GWAS datasets were imputed into the NIH Trans-Omics for Precision Medicine (TOPMed) release2 from BioData Catalyst. Furthermore, we performed fine-mapping, trans-ethnic meta-analysis, and in silico functional characterization to identify likely causal variants with a functional interpretation. Results: We observed genome-wide significant (P-value < 5.0E−8) SNPs associations near AADACL2 and miRNA (MIR5186) genes in chromosome 3 after adjusting for hypertension, diabetes, dyslipidemia, and cardiac status in the base model as covariates. SNPs near the miRNA (MIR4458) gene in chromosome 5 were also associated with stroke (P-value < 1.0E−6). The putative genes near AADACL2, MIR5186, and MIR4458 genes were protective and novel. SNPs associations with stroke in chromosome 2 were more than 77 kb from the closest gene LINC01854 and SNPs in chromosome 7 were more than 116 kb to the closest gene LINC01446 (P-value < 1.0E−6). In addition, we observed SNPs in genes STXBP5-AS1 (chromosome 6), GALTN9 (chromosome 12), FANCA (chromosome 16), and DLGAP1 (chromosome 18) (P-value < 1.0E−6). Both genomic regions near genes AADACL2 and MIR4458 remained significant following fine mapping. Conclusions: Our findings identify potential roles of regulatory miRNA, intergenic non-coding DNA, and intronic non-coding RNA in the biology of ischemic stroke. These findings reveal new molecular targets that promise to help close the current gaps in accurate African ancestry-based genetic stroke’s risk prediction and development of new targeted interventions to prevent or treat stroke

Perception of Farm Succession Planning by Poultry Farmers in Southwest, Nigeria

This study assessed poultry farm characteristics and poultry farmers’ perception of farm succession planning in southwest Nigeria.  A multistage sampling procedure was used in selecting poultry farmers in Oyo and Osun states.  Data were analyzed using descriptive and inferential statistics. Results reveal that poultry farmers are predominantly small and medium scale farmers. They are mostly sole proprietors (72.5%) and family farm owners (11.9 %).  Most (73.4%) farmers operated on full time basis, while others are either part – time or occasional farmers.  The majority (95.9%) of the farmers had favourable perception towards farm succession planning, 30.3% had complete succession plan and 22.9% had commenced successor participation. There were no significant associations between farm ownership structure, but significant associations exist with farming status (χ2 = 9.7), total farm debt (χ2 = 19.2), and succession planning among the poultry farmers (p≤ 0.05). The study concluded that succession planning is new in the study area and must be further investigated in order to provide a data base for policy decision with regards to livestock (especially poultry) farming and aging proprietorship.Â

Perception of Farm Succession Planning by Poultry Farmers in Southwest, Nigeria

This study assessed poultry farm characteristics and poultry farmers’ perception of farm succession planning in southwest Nigeria.  A multistage sampling procedure was used in selecting poultry farmers in Oyo and Osun states.  Data were analyzed using descriptive and inferential statistics. Results reveal that poultry farmers are predominantly small and medium scale farmers. They are mostly sole proprietors (72.5%) and family farm owners (11.9 %).  Most (73.4%) farmers operated on full time basis, while others are either part – time or occasional farmers.  The majority (95.9%) of the farmers had favourable perception towards farm succession planning, 30.3% had complete succession plan and 22.9% had commenced successor participation. There were no significant associations between farm ownership structure, but significant associations exist with farming status (χ2 = 9.7), total farm debt (χ2 = 19.2), and succession planning among the poultry farmers (p≤ 0.05). The study concluded that succession planning is new in the study area and must be further investigated in order to provide a data base for policy decision with regards to livestock (especially poultry) farming and aging proprietorship.Â

Chemical composition, storage stability and effect of cold-pressed flaxseed oil cake inclusion on bread quality

Abstract Flaxseed oil cake from a South African factory was screened for proximate composition, mineral content, fatty acid profile and storage stability. The oil cake was included at 10 and 15% levels (w/w) in brown bread and evaluated using a 96-member consumer panel. The oil cake contained between 38.0 and 47.3% protein, 12.8 and 26.1% crude fat and 3.7 and 5.1% ash. The total carbohydrates were mostly dietary fiber. Calcium, magnesium, phosphorus and potassium were in the range of 3.3 to 3.8, 4.8 to 5.9, 6.4 to 8.2 and 9.0 to 10.1 (mg/g), respectively. The oil from the flaxseed oil cake contained 58.5 to 59.7% of C18 omega-3 fatty acids. Peroxide levels of the flaxseed oil cake were below the threshold limits after 6 months storage. Thiobarbituric acid threshold values were exceeded after 5 months aerobic storage at 20C. Bread samples with inclusion levels of 10 and 15% flaxseed oil cake were acceptable to the consumer sensory panel

Human Rights Implications of Stroke Biobanking and Genomics Research in Sub-Saharan Africa

\ua9 2022. Abstract: Stroke is a major cause of death in Sub-Saharan Africa (SSA) and genetic factors appear to play a part in its pathogenesis. This led to the development of stroke biobanking and genomics research in SSA. Existing stroke studies have focused on causes, incidence rates, fatalities and effects. However, scant attention has been paid to the legal issues about stroke biobanking and genomics research in the sub-region. Therefore, this article examines the legal implications of stroke biobanking and genomics research in Sub-Saharan Africa from a human rights perspective. The study argues that the right to dignity of the human person, the right to privacy, the right to freedom of information, the right to freedom from discrimination, the right to own property, the right to self-determination and the right to health may be implicated. The study concludes that the court may have to be involved in balancing one right against the other which may prove somewhat herculean depending on the circumstances of each case

Legal Implications of Stroke Biobanking and Genomics Research in Sub-Saharan Africa

Stroke is a major cause of death in Sub-Saharan Africa (SSA) and genetic factors appear to play a part. This has led to stroke biobanking and genomics research in SSA. Existing stroke studies have focused on causes, incidence rates, fatalities and effects. However, scant attention has been paid to the legal issues in stroke biobanking and genomics research in the sub-region. Therefore, this article examines the legal implications of stroke biobanking and genomics research in SSA. The article adopts a textual analysis of primary and secondary sources in law. It reports that there are laws from the perspectives of human right, the common law, and intellectual property. However, there are gaps to be filled. The article therefore argues for legislative intervention. It concludes that pending the time the statute will be enacted, genomics researchers in Africa should adopt the ethical guidelines prepared by Human Heredity and Health in Africa (H3 Africa)

Symbolic legislation and the regulation of stroke biobanking and genomics research in Sub-Saharan Africa

\ua9 2022 Informa UK Limited, trading as Taylor & Francis Group. Stroke is a major cause of death in Sub-Saharan Africa (SSA) and genetic factors appear to play a part. This led to the development of stroke bio-banking and genomics research in SSA. Existing stroke studies have focused on causes, incidence rates, fatalities and effects. However, scant attention has been paid to the legal issues about stroke bio-banking and genomics research in the sub-region. Therefore, this article examines how genomics research and stroke bio-banking in SSA can be regulated through legislation. The article reports that there are germane issues to be addressed such as appropriate consent model, commercial use of biological samples, ownership right in biological samples and return of research results but that the position of the law on these issues is not satisfactory because there is no statute directly regulating them while existing regulations in these countries are either absent, outdated, conservative or difficult to navigate. The article, therefore, applies the theory of symbolic legislation and argues for legislative intervention through a positive symbolic approach. It recommends that the statute to be enacted should only address policy issues by way of legal rules without being detailed while the understanding of the rules should be fostered in explanatory notes. The explanatory notes should contain examples borne of decided cases, cases settled out of court and the ethical guidelines prepared by Human Heredity and Health in Africa (H3 Africa). Where they are inadequate, recourse may be had to other ethical guidelines subject to the demands of local circumstances

Capacity-Building for Stroke Genomic Research Data Collection: The African Neurobiobank Ethical, Legal, and Social Implications Project Experience

\ua9 2023 Mary Ann Liebert, Inc., publishers. Background: The fields of stroke genomics, biobanking, and precision medicine are rapidly expanding in sub-Saharan Africa. However, the ethical, legal, and social implications (ELSI) of emerging neurobiobanking and genomic data resources are unclear in an emerging African scientific landscape with unique cultural, linguistic, and belief systems. Objective: This article documents capacity-building experiences of researchers during the development, pretesting, and validation of data collection instruments of the African Neurobiobank for Precision Stroke Medicine - (ELSI) Project. Methods: The African Neurobiobank for Precision Stroke Medicine - ELSI project is a transnational, multicenter project implemented across seven sites in Ghana and Nigeria. Guided by the Community-Based Participatory Research framework, we conducted three workshops with key stakeholders to review the study protocol, ensure uniformity in implementation; pretest, harmonize, and integrate context-specific feedback to ensure validity and adaptability of data collection instruments. Workshop impact was assessed using an open-ended questionnaire, which included questions on experience with participation in any of the workshops, building capacity in Genetic and Genomic Research (GGR), level of preparedness toward GGR, the genomic mini-dictionary developed by the team, and its impact in enhancing understanding in GGR. Data were analyzed qualitatively using a thematic framework approach. Results: Findings revealed the usefulness of the workshop in improving participants\u27 knowledge and capacity toward GGR implementation. It further identified local, context-specific concerns regarding quality data collection, the need to develop culturally acceptable, genomic/biobanking data collection tools, and a mini-dictionary. Participants-reported perceptions were that the mini-dictionary enhanced understanding, participation, and data collection in GGR. Overall, participants reported increased preparedness and interest in participating in GGR. Conclusion: Capacity-building is a necessary step toward ELSI-related genomic research implementation in African countries where scholarship of ELSI of genomics research is emerging. Our findings may be useful to the design and implementation of ELSI-GGR projects in other African countries