MORPHOFUNCTIONAL ESTIMATION OF IMMUNE FUNCTION OF LYMPH NODE OF OLD AGE IN THE CONDITIONS OF PHYTOTHERAPY

The morphofunctional estimation of the immune status of a lymph node of animals of old. age is spent in experiment. Data has shown decrease in immune function of the lymph node because of a fibrosis process of cells depletion of paracortex structure in the conditions of a lymphopoiesis reduction. The ageing strengthens the cellular immunity that is characterized by increase in paracortex size at preservation of a parity of lymphoid nodules with the germinative centre and without the germinative centre. The phytotherapy strengthens proliferative processes accompanied by increase of number of cells in lymphoid nodules, stimulates of macrophage reaction and increases number of plasmablasts in all structurally and functional zones of a lymph node of animals of old age. There is a formation of the immune answer on the mixed type in the conditions of phytotherapy. The result has practical importance for use of the immunefocused phytotherapy in programs of endoecological rehabilitations taking into account the age facto

MORPHOLOGICAL CHARACTERISTICS OF TISSUE MICROREGION OF THE THYROID GLAND AT EXPERIMENTAL HYPOTHYROIDISM

Research purpose was to study the structures of the tissue microregion of the thyroid gland at an experimental hypothyroidism. Material and methods. The hypothyroidism model was created by mercazolilum intake at a dose of 5 mg/100 g of body mass for a month. The structure of tissue area (follicle, thyrocyte, interfollicular space) with morphometry and calculation of the integral indicators of the thyroid gland functional activity have been investigated by the light-optical and ultrastructural methods. Results and discussion. The morphophysiological structure of the thyroid gland is a tissue microregion. It unites a group of follicles and interfollicular space. The structure of the tissue miroregion have features of structural response to merkazolilum intake. The thyrostatic remedy changes the follicular organization of the thyroid gland to the predominance of small and medium follicles with signs of alteration. Thyrocytes have expansion and deformation of the granular endoplasmic reticulum cisterns, formation of intracellular colloid, reduction in the number of lysosomes and mitochondrions with their disorganization. The ratio of the area of the follicular epithelium, colloid and stroma changes at hypothyroidism. The portion of interstitial increases, there is an accumulation of tissue fluid because of weakening of a lymphatic drainage. Blood and lymphatic microvessels react by reducing the volume density to mercazolilum intake. Conclusion. The merkazolilum thyreostatics effects on morphology and metabolic and transport processes in tissue microregion. It testifies to decrease of thyroid function and the creation of hypothyroidism adequate model

Pharmacological properties of selenium and its preparations: from antioxidant to neuroprotector

Selenium is an essential component of more than two dozen enzymes and other selenoproteins that play critical roles in reproduction, DNA synthesis, thyroid hormone metabolism, and protection from oxidative damage and infection. In this review, we want to emphasize pharmacological role of Selenium and its derivatives on human health is very complex and has yet to be fully understoo

Determination of ππ\pi\pi scattering lengths from measurement of π+π−\pi^+\pi^- atom lifetime

The DIRAC experiment at CERN has achieved a sizeable production of $\pi^+\pi^-$ atoms and has significantly improved the precision on its lifetime determination. From a sample of 21227 atomic pairs, a 4% measurement of the S-wave $\pi\pi$ scattering length difference $|a_0-a_2| = (.0.2533^{+0.0080}_{-0.0078}|_\mathrm{stat}.{}^{+0.0078}_{-0.0073}|_\mathrm{syst})M_{\pi^+}^{-1}$ has been attained, providing an important test of Chiral Perturbation Theory.Comment: 6 pages, 6 figure

Comparative analysis of lactaptin activity when produced in bacterial or eukaryotic expression systems

Despite the multitude of anticancer cytostatic drugs available to oncologists today, most of such drugs have serious side effects that may preclude their use in some groups of patients. Hence, selective induction of apoptosis in cancer but not normal cells remains an attractive goal of molecular medicine. Lactaptin, a proteolytic fragment of the human milk kappa-casein, has been previously identified as a protein displaying potent killing of cancer cells in vitro. Its recombinant analog (RL2) produced in E. coli has been shown to delay solid tumor growth in vivo. Given that lactaptin is of human origin and is not immunogenic, it can be administered to patients multiple times without running the risk of immune response that could dampen the therapy efficacy. In the present study, we demonstrate that the combination of RL2 and cyclophosphamide treatments has an additive therapeutic effect against hepatoma tumor in immunocompetent mice. We asked whether production of lactaptin in human rather than bacterial cells would result in a protein with increased cytotoxic activity. Using lentiviral vector pCDH as a backbone, two constructs, pEL1 and pEL2, encoding secreted forms of lactaptin that differ in their signal sequences were created. Lactaptin expression in human cell lines was confirmed using Western-blot analysis, whereas ELISA was used for quantification of secreted lactaptin. Next, we measured the cytotoxic effects of the media conditioned by pEL1-transfected HEK293T cells, as assayed against the panel of three human cancer cell lines: MDA-MB-231 (adenocarcinoma), PC3 (prostate cancer), and T98G (glioblastoma). We show that EL1-derived lactaptin is at least 100-fold more cytotoxic than RL2. Taken together, our results provide an opportunity for developing armored immune cells as an “off-the-shelf” platform for targeted delivery of lactaptin to cancer cells

First πK\pi K atom lifetime and πK\pi K scattering length measurements

The results of a search for hydrogen-like atoms consisting of $\pi^{\mp}K^{\pm}$ mesons are presented. Evidence for $\pi K$ atom production by 24 GeV/c protons from CERN PS interacting with a nickel target has been seen in terms of characteristic $\pi K$ pairs from their breakup in the same target ($178 \pm 49$) and from Coulomb final state interaction ($653 \pm 42$). Using these results the analysis yields a first value for the $\pi K$ atom lifetime of $\tau=(2.5_{-1.8}^{+3.0})$ fs and a first model-independent measurement of the S-wave isospin-odd $\pi K$ scattering length $\left|a_0^-\right|=\frac{1}{3}\left|a_{1/2}-a_{3/2}\right|= \left(0.11_{-0.04}^{+0.09} \right)M_{\pi}^{-1}$ ($a_I$ for isospin $I$).Comment: 14 pages, 8 figure

Detection of π+π−\pi^+\pi^-atoms with the DIRAC spectrometer at CERN

The goal of the DIRAC experiment at CERN is to measure with high precision the lifetime of the $\pi^+\pi^-$ atom ($A_{2\pi}$), which is of order $3\times10^{-15}$ s, and thus to determine the s-wave $\pi\pi$-scattering lengths difference $|a_{0}-a_{2}|$. $A_{2\pi}$ atoms are detected through the characteristic features of $\pi^+\pi^-$ pairs from the atom break-up (ionization) in the target. We report on a first high statistics atomic data sample obtained from p Ni interactions at 24 GeV/$c$ proton momentum and present the methods to separate the signal from the background.Comment: 19 pages, 12 figures, 1 tabl

Evidence for πK\pi K-atoms with DIRAC

We present evidence for the first observation of electromagnetically bound $\pi^\pm K^\mp$-pairs ($\pi K$-atoms) with the DIRAC experiment at the CERN-PS. The $\pi K$-atoms are produced by the 24 GeV/c proton beam in a thin Pt-target and the $\pi^\pm$ and $K^\mp$-mesons from the atom dissociation are analyzed in a two-arm magnetic spectrometer. The observed enhancement at low relative momentum corresponds to the production of 173 $\pm$ 54 $\pi K$-atoms. The mean life of $\pi K$-atoms is related to the s-wave $\pi K$-scattering lengths, the measurement of which is the goal of the experiment. From these first data we derive a lower limit for the mean life of 0.8 fs at 90% confidence level.Comment: 15 pages, 9 figure

SH3 Domain-Mediated Recruitment of Host Cell Amphiphysins by Alphavirus nsP3 Promotes Viral RNA Replication

Among the four non-structural proteins of alphaviruses the function of nsP3 is the least well understood. NsP3 is a component of the viral replication complex, and composed of a conserved aminoterminal macro domain implicated in viral RNA synthesis, and a poorly conserved carboxyterminal region. Despite the lack of overall homology we noted a carboxyterminal proline-rich sequence motif shared by many alphaviral nsP3 proteins, and found it to serve as a preferred target site for the Src-homology 3 (SH3) domains of amphiphysin-1 and -2. Nsp3 proteins of Semliki Forest (SFV), Sindbis (SINV), and Chikungunya viruses all showed avid and SH3-dependent binding to amphiphysins. Upon alphavirus infection the intracellular distribution of amphiphysin was dramatically altered and colocalized with nsP3. Mutations in nsP3 disrupting the amphiphysin SH3 binding motif as well as RNAi-mediated silencing of amphiphysin-2 expression resulted in impaired viral RNA replication in HeLa cells infected with SINV or SFV. Infection of Balb/c mice with SFV carrying an SH3 binding-defective nsP3 was associated with significantly decreased mortality. These data establish SH3 domain-mediated binding of nsP3 with amphiphysin as an important host cell interaction promoting alphavirus replication

The bound mu+ mu- system

We consider the hyperfine structure, the atomic spectrum and the decay channels of the bound mu+ mu- system (dimuonium). The annihilation lifetimes of low-lying atomic states of the system lie in the nanosecond range range. The decay rates could be measured by detection of the decay products (high energy photons or electron-positron pairs). The hyperfine structure splitting of the dimuonic system and its decay rate are influenced by electronic vacuum polarization effects in the far time-like asymptotic region. This constitutes a previously unexplored kinematic regime. We evaluate next--to-leading order radiative corrections to the decay rate of low-lying atomic states. We also obtain order alpha^5 corrections to the hyperfine splitting of the 1S and 2S levels.Comment: 10 figures (eps format) attached, Scheduled tentatively by PRA for Nov/Dec 199