Determination of As levels in livers of pregnant dams and GD18 fetuses after 10 days exposure to 49 ppm As (as NaAsO2) in drinking water.

<p>Means±SD. P<0.01.</p

Plasma ALT and AST in arsenic-exposed and -unexposed PND70 ApoE-knockout mice.

<p>Mean±S.E. n = 8−9 (*p<0.026; #p = 0.056).</p

Identification and analyses of 51 genes differentially expressed at both PND1 and PND70.

<p>A. Intersection of genes differentially expressed at PND1 and PND70. B. Hierarchical clustering of 51 genes differentially expressed at both PND 1 and PND70. C. Principal component analysis of 51 genes differentially expressed at both PND 1 and PND70. (Arrows added for clarity) D. Pathway Architect analysis of 51 genes differentially expressed at both PND 1 and PND70.</p

Diagram of experimental design and analytical flow for mRNA and miRNA data.

<p>Diagram of experimental design and analytical flow for mRNA and miRNA data.</p

Cluster maps of enriched transcription factor binding sites (TFBS) in promoters of genes that are differentially expressed at PND1 (101 genes) and PND70 (135 genes) and also are targets of differentially expressed microRNAs at PND1 (Table S6) and PND70 (Table S11) respectively.

<p>Cluster maps of enriched transcription factor binding sites (TFBS) in promoters of genes that are differentially expressed at PND1 (101 genes) and PND70 (135 genes) and also are targets of differentially expressed microRNAs at PND1 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038713#pone.0038713.s008" target="_blank">Table S6</a>) and PND70 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038713#pone.0038713.s013" target="_blank">Table S11</a>) respectively.</p

Transcription factor binding sites enriched in gene promoters of differentially expressed mRNAs that are targets of differentially expressed microRNAs in PND1 mice.

<p>A total of 101 unique Entrez gene IDs are gene targets of induced or suppressed microRNAs and appear in the gene list of differentially expressed mRNAs at PND1. A total 17 transcription factors are enriched for this gene set with a P-value <0.05.</p

Gene ontology of differentially expressed microRNA target mRNAs.

<p>Gene ontology of differentially expressed microRNA target mRNAs.</p

microRNAs differentially expressed at PND1 and PND70.

<p>microRNAs differentially expressed at PND1 and PND70.</p

HSP70 and SREBP1 expression in livers of 10 week old mice.

<p>A. Western blot analysis of HSP70 and SREBP1. The SREBP1 fragment shown is the 68 kDa cleavage fragment. B. Densitometric quantitation of western blots. Signals for each protein were normalized to GAPDH and mean of controls (#’s 1–4) was set = 1. Representative of triplicate blots shown. * = p<0.05.</p

Interactome maps of differentially expressed mRNAs in livers of arsenic-exposed mice.

<p>A. PND1; B. PND70. Major hubs are annotated.</p