60 research outputs found
Experimental observation of superradiance in millimeter-wave band
The first experimental results of the observation of superradiance from a single subnanosecond electron bunch are presented. Superradiance was associated with different varieties of stimulated emission (bremstruhlung, cyclotron, Cherenkov, etc). Unique megawatt power level microwave pulses of short duration (0.3-0.5 ns) have been obtained
Differential impact of LPG-and PG-deficient Leishmania major mutants on the immune response of human dendritic cells
<div><p>Background</p><p><i>Leishmania major</i> infection induces robust interleukin-12 (IL12) production in human dendritic cells (hDC), ultimately resulting in Th1-mediated immunity and clinical resolution. The surface of <i>Leishmania</i> parasites is covered in a dense glycocalyx consisting of primarily lipophosphoglycan (LPG) and other phosphoglycan-containing molecules (PGs), making these glycoconjugates the likely pathogen-associated molecular patterns (PAMPS) responsible for IL12 induction.</p><p>Methodology/Principal Findings</p><p>Here we explored the role of parasite glycoconjugates on the hDC IL12 response by generating <i>L</i>. <i>major</i> Friedlin V1 mutants defective in LPG alone, (FV1 <i>lpg1-</i>), or generally deficient for all PGs, (FV1 <i>lpg2-</i>). Infection with metacyclic, infective stage, <i>L</i>. <i>major</i> or purified LPG induced high levels of <i>IL12B</i> subunit gene transcripts in hDCs, which was abrogated with FV1 <i>lpg1-</i> infections. In contrast, hDC infections with FV1 <i>lpg2-</i> displayed increased <i>IL12B</i> expression, suggesting other PG-related/<i>LPG2</i> dependent molecules may act to dampen the immune response. Global transcriptional profiling comparing WT, FV1 <i>lpg1-</i>, FV1 <i>lpg2-</i> infections revealed that FV1 <i>lpg1-</i> mutants entered hDCs in a silent fashion as indicated by repression of gene expression. Transcription factor binding site analysis suggests that LPG recognition by hDCs induces IL-12 in a signaling cascade resulting in Nuclear Factor Îș B (NFÎșB) and Interferon Regulatory Factor (IRF) mediated transcription.</p><p>Conclusions/Significance</p><p>These data suggest that <i>L</i>. <i>major</i> LPG is a major PAMP recognized by hDC to induce IL12-mediated protective immunity and that there is a complex interplay between PG-baring <i>Leishmania</i> surface glycoconjugates that result in modulation of host cellular IL12.</p></div
Six-Coordinate Nitrito and Nitrato Complexes of Manganese Porphyrin
Reaction of small increments of NO2 gas with sublimed amorphous layers of Mn(II)(TPP) (TPP = meso-tetra-phenylporphyrinato dianion) in a vacuum cryostat leads to formation of the 5-coordinate monodentate nitrato complex Mn(III)(TPP)(η(1)-ONO2) (II). This transformation proceeds through the two distinct steps with initial formation of the five coordinate O-nitrito complex Mn(III)(TPP)(η(1)-ONO) (I) as demonstrated by the electronic absorption spectra and by FTIR spectra using differently labeled nitrogen dioxide. A plausible mechanism for the second stage of reaction is offered based on the spectral changes observed upon subsequent interaction of (15)NO2 and NO2 with the layered Mn(TPP). Low-temperature interaction of I and II with the vapors of various ligands L (L = O-, S-, and N-donors) leads to formation of the 6-coordinate O-nitrito Mn(III)(TPP)(L)(η(1)-ONO) and monodentate nitrato Mn(III)(TPP)(L)(η(1)-ONO2) complexes, respectively. Formation of the 6-coordinate O-nitrito complex is accompanied by the shifts of the Îœ(NâO) band to lower frequency and of the Îœ(N-O) band to higher frequency. The frequency difference between these bands ÎÎœ = Îœ(NâO) - Îœ(N-O) is a function of L and is smaller for the stronger bases. Reaction of excess NH3 with I leads to formation of Mn(TPP)(NH3)(η(1)-ONO) and of the cation [Mn(TPP)(NH3)2](+) plus ionic nitrite. The nitrito complexes are relatively unstable, but several of the nitrato species can be observed in the solid state at room temperature. For example, the tetrahydrofuran complex Mn(TPP)(THF)(η(1)-ONO2) is stable in the presence of THF vapors (âŒ5 mm), but it loses this ligand upon high vacuum pumping at RT. When L = dimethylsulfide (DMS), the nitrato complex is stable only to âŒ-30 °C. Reactions of II with the N-donor ligands NH3, pyridine, or 1-methylimidazole are more complex. With these ligands, the nitrato complexes Mn(III)(TPP)(L)(η(1)-ONO2) and the cationic complexes [Mn(TPP)(L)2](+) coexist in the layer at room temperature, the latter formed as a result of NO3(-) displacement when L is in excess
Study of the structure and properties of FeâNiâCoâ(Cu, Cr) alloys using a combination of experimental methods
Abstract: The structure and properties of metallic multicomponent FeâNiâCoâCrâCu and FeâNiâCoâCuâCr system alloys (x is the component content, at %), which are the basis of the wide class of high-entropy alloys, are studied. The synthesized alloys are investigated using instrumental analysis methods (DTA, SEM-EPMA, XRD), and the concentration and temperature boundaries of the fcc solid solution have been determined
Experimental observation of Cherenkov superradiance from an intense electron bunch
Experimental results are presented of the observation of stimulated Cherenkov radiation when intense subnanosecond electron bunches were moving through a dielectric-loaded waveguide. The ultrashort duration microwave pulses (up to 400 ps which is equivalent to similar to 10 periods of the radiation) in combination with specific dependencies of peak microwave power on interaction length demonstrate that this emission can be interpreted as coherent superradiant (SR) emission. The peak power associated with the SR emission was similar to 1-2 MW. The spectrum of radiation belongs to the frequency range 38-48 GHz. The experimental results are compared with theoretical analyses
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