608 research outputs found
Development of microfluidic bubble perfusion device for neuropeptide analysis from ex vivo brain slice models
Expanding the CRISPR toolbox in Culicine mosquitoes: in vitro validation of Pol III promoters
CRISPRâCas9-based âgene driveâ technologies have been proposed as a novel and effective means of controlling human diseases vectored by mosquitoes. However, more complex designs than those demonstrated to dateâand an expanded molecular toolbox with which to build themâwill be required to overcome the issues of resistance formation/evolution and drive spatial/temporal limitation. Foreseeing this need, we assessed the sgRNA transcriptional activities of 33 phylogenetically diverse insect Polymerase III promoters using three disease-relevant Culicine mosquito cell lines (Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus). We show that U6 promoters work across species with a range of transcriptional activity levels and find 7SK promoters to be especially promising because of their broad phylogenetic activity. We further show that U6 promoters can be substantially truncated without affecting transcriptional levels. These results will be of great utility to researchers involved in developing the next generation of gene drives
Behavioural effects of juvenile hormone and their influence on division of labour in leaf-cutting ant societies
Division of labour in social insects represents a major evolutionary transition, but the physiological mechanisms that regulate this are still little understood. Experimental work with honey bees, and correlational analyses in other social insects, have implicated juvenile hormone (JH) as a regulatory factor, but direct experimental evidence of behavioural effects of JH in social insects is generally lacking. Here, we used experimental manipulation of JH to show that raised JH levels in leaf-cutting ants results in workers becoming more active, phototactic and threat responsive, and engaging in more extranidal activity â behavioural changes that we show are all characteristic of the transition from intranidal work to foraging. These behavioural effects on division of labour suggest that the JH mediation of behaviour occurs across multiple independent evolutions of eusociality, and may be a key endocrine regulator of the division of labour which has produced the remarkable ecological and evolutionary success of social insects
Antidepressant Dosing in Major Depression: A Pharmacogenomic Approach
Major depressive disorder (MDD) is the most predominant mental disorder in the United States, with serious and costly health risks if not successfully managed. Pharmacotherapy is a standard option for MDD treatment, but patients often require extensive therapy adjustments to find a suitable regimen. Pharmacogenomics may enable greater precision in antidepressant therapy. Genotypic variations in CYP2D6 and CYP2C19 metabolic enzymes are reliable predictors of serum drug concentration, but the complex dose-response relationship of antidepressants prevents such variations from predicting therapy success. Additionally, ABCBl has been examined for its role in P-glycoprotein efflux of antidepressants in the brain, yet it is still inconclusive as to which variations are correlated with drug response. Current genotypic guidelines are largely proactive and clinical trials utilizing genotypic dosing have shown significant reductions in side effects and health care costs. Further studies of genotypic targets are needed and, if the possible clinical benefits are confirmed, the use of genotyping will be an important tool in optimizing antidepressant therapy
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Caste and task allocation in ants
Group living is a widely adopted strategy by many organisms and given the advantages offered by a social lifestyle, such as increased protection from predators or increased ability for resource exploitation, a wide variety of animals have adopted a social lifestyle. Arguably none have done this more successfully than the social insects. Indeed their efficient division of labour is often cited as a key attribute for the remarkable ecological and evolutionary success of these societies. Within the social insects the most obvious division of labour is reproductive, in which one or a few individuals monopolise reproduction while the majority of essentially sterile workers carry out the remaining tasks essential for colony survival. In almost all social insects, in particular ants, the age of a worker will predispose it to certain tasks, and in some social insects the workers vary in size such that task is associated with worker morphology. In this thesis I explore the proximate and ultimate causes of worker and reproductive division of labour in ant societies, which span a range of social complexities. I predominantly focus on both the highly derived leaf-cutting ants â a so-called âpinnacleâ of evolution within the social insects, with a complex division of labour and a strong worker caste system â and in the more basal primitive societies of the queenless ponerine dinosaur ants, which can offer an insight in to the evolution of division of labour at the earliest stages of social lifestyles. This work demonstrates the environmental and genetic determinants of division of labour in group-living societies outside of the classical honey bee model system. This is important as it helps us to better understand the broader processes shaping behaviour and phenotype in the animal kingdom
Evaluation of fentanyl immunoassay test strips for rapid in-situ detection of fentanyl and fentanyl analogs in seized samples and alternative matrices
The mechanics of solid-state nanofoaming.
Solid-state nanofoaming experiments are conducted on two polymethyl methacrylate (PMMA) grades of markedly different molecular weight using CO2 as the blowing agent. The sensitivity of porosity to foaming time and foaming temperature is measured. Also, the microstructure of the PMMA nanofoams is characterized in terms of cell size and cell nucleation density. A one-dimensional numerical model is developed to predict the growth of spherical, gas-filled voids during the solid-state foaming process. Diffusion of CO2 within the PMMA matrix is sufficiently rapid for the concentration of CO2 to remain almost uniform spatially. The foaming model makes use of experimentally calibrated constitutive laws for the uniaxial stress versus strain response of the PMMA grades as a function of strain rate and temperature, and the effect of dissolved CO2 is accounted for by a shift in the glass transition temperature of the PMMA. The maximum achievable porosity is interpreted in terms of cell wall tearing and comparisons are made between the predictions of the model and nanofoaming measurements; it is deduced that the failure strain of the cell walls is sensitive to cell wall thickness
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