Late Pleistocene to early-Holocene rainforest foraging in Sri Lanka:Multidisciplinary analysis at Kitulgala Beli-lena

Sri Lanka has produced the earliest clear evidence for Homo sapiens fossils in South Asia and research in the region has provided important insights into modern human adaptations and cultural practices during the last ca. 45,000 years. However, in-depth multidisciplinary analyses of Late Pleistocene and Holocene sequences remain limited to just two sites, Fa Hien-lena and Batadomba-lena. Here, we present our findings from the reinvestigation of a third site, Kitulgala Beli-lena. New chronometric dating from the site confirms the presence of humans as early as ca. 45,000 cal. BP. in the island's Wet Zone rainforest region. Our analyses of macrobotanical, molluscan, and vertebrate remains from the rockshelter show that this early human presence is associated with rainforest foraging. The Late Pleistocene deposits yielded evidence of wild breadfruit and kekuna nut extraction while the Holocene layers reveal a heavy reliance on semi-arboreal and arboreal small mammals as well as freshwater snails as a protein source. The lithic and osseous artefacts demonstrate that populations developed a sophisticated tool kit for the exploitation of their immediate landscapes. We place the rich Kitulgala Beli-lena dataset in its wider Sri Lankan context of Late Pleistocene foraging, as well as in wider discussions of our species’ adaptation to ‘extreme’ environments as it moved throughout Asia

Land Use Change in a Pericolonial Society: Intensification and Diversification in Ifugao, Philippines Between 1570 and 1800 CE

Land use modelling is increasingly used by archaeologists and palaeoecologists seeking to quantify and compare the changing influence of humans on the environment. In Southeast Asia, the intensification of rice agriculture and the arrival of European colonizers have both been seen as major catalysts for deforestation, soil erosion, and biodiversity change. Here we consider the Tuwali-Ifugao people of the Cordillera Central (Luzon, Philippines), who resisted Spanish colonial subjugation from the 16th to the mid-nineteenth century, in part through the development of a world-renowned system of intensive wet-rice terrace agriculture. To quantify changes in how the Tuwali-Ifugao used their environment, we model land use in Old Kiyyangan Village, a long-inhabited settlement, at two timepoints: circa 1570 CE, prior to the Spanish arrival in Luzon, and circa 1800 CE, before the village was sacked by Spanish military expeditions. Our model demonstrates that between 1570 and 1800 the adoption of rice as a staple and the corresponding expansion in terrace agriculture, along with a general diversification of diet and land use, enabled the village’s population to double without increasing total land use area. Further, this major intensification led to the solidification of social hierarchies and occurred without a proportional increase in deforestation

The invisible Plant Technology of Prehistoric Southeast Asia: Indirect Evidence for Basket and Rope Making at Tabon Cave, Philippines, 39-33,000 Years Ago.

A large part of our material culture is made of organic materials, and this was likely the case also during prehistory. Amongst this prehistoric organic material culture are textiles and cordages, taking advantage of the flexibility and resistance of plant fibres. While in very exceptional cases and under very favourable circumstances, fragments of baskets and cords have survived and were discovered in late Pleistocene and Holocene archaeological sites, these objects are generally not preserved, especially in tropical regions. We report here indirect evidence of basket/tying material making found on stone tools dating to 39–33,000 BP from Tabon Cave, Palawan Philippines. The distribution of use-wear on these artefacts is the same as the distribution observed on experimental tools used to thin fibres, following a technique that is widespread in the region currently. The goal of this activity is to turn hard plant segments into supple strips suitable as tying material or to weave baskets, traps, and even boats. This study shows early evidence of this practice in Southeast Asia and adds to the growing set of discoveries showing that fibre technology was an integral part of late Pleistocene skillset. This paper also provides a new way to identify supple strips of fibres made of tropical plants in the archaeological record, an organic technology that is otherwise most of the time invisible

Specialized rainforest hunting by Homo sapiens ~45,000 years ago

Defining the distinctive capacities of Homo sapiens relative to other hominins is a major focus for human evolutionary studies. It has been argued that the procurement of small, difficult-to-catch, agile prey is a hallmark of complex behavior unique to our species; however, most research in this regard has been limited to the last 20,000 years in Europe and the Levant. Here, we present detailed faunal assemblage and taphonomic data from Fa-Hien Lena Cave in Sri Lanka that demonstrates specialized, sophisticated hunting of semi-arboreal and arboreal monkey and squirrel populations from ca. 45,000 years ago, in a tropical rainforest environment. Facilitated by complex osseous and microlithic technologies, we argue these data highlight that the early capture of small, elusive mammals was part of the plastic behavior of Homo sapiens that allowed it to rapidly colonize a series of extreme environments that were apparently untouched by its hominin relatives

Excavations at the site of Vasino, Lautem District, Timor-Leste

This chapter explores the archaeology and ethnohistory of one of the distinctive fortified settlements in the eastern part of Timor-Leste. In 2009, a team from The Australian National University (ANU), together with local people, partially excavated the site of Vasino, located close to the north coast of Timor-Leste, above the modern village of Moro-Parlamento (Figure 4.1). The site had been fortified with large stone walls and the aim was to provide more data on when, how and why these fortifications were used in the region. Two related questions guided the research. First, when was the main period of fort construction initiated? Secondly, what were the prevailing environmental and social conditions of those times

A new legacy: potential of zooarchaeology by mass spectrometry in the analysis of North American megafaunal remains

Museum legacy collections, often derived from large-scale archaeological excavations, can serve as paleoenvironmental archives of Late Pleistocene megafaunal composition and dynamics. Many of these collections, however, contain large quantities of highly fragmented and morphologically indistinct bones that cannot be identified to a specific taxon and are therefore of limited use to paleoenvironmental and archaeological analyses. Here, we explore the potential of Zooarchaeology by Mass Spectrometry (ZooMS) to identify fossil bone fragments and complement morphological identifications in legacy collections housed at the Smithsonian’s National Museum of Natural History. To undertake this work, we collected fragmented bone specimens of Late Pleistocene megafauna from six archaeological sites in Colorado that are currently housed in the Department of Anthropology, and then performed pilot ZooMS screening. Our analysis successfully retrieved taxonomic information from 80% of the analyzed material, highlighting the potential of future ZooMS studies on museum collections to investigate human-megafaunal interactions in late Pleistocene North America

Earliest known human burial in Africa

The origin and evolution of hominin mortuary practices are topics of intense interest and debate1,2,3. Human burials dated to the Middle Stone Age (MSA) are exceedingly rare in Africa and unknown in East Africa1,2,3,4,5,6. Here we describe the partial skeleton of a roughly 2.5- to 3.0-year-old child dating to 78.3 ± 4.1 thousand years ago, which was recovered in the MSA layers of Panga ya Saidi (PYS), a cave site in the tropical upland coast of Kenya7,8. Recent excavations have revealed a pit feature containing a child in a flexed position. Geochemical, granulometric and micromorphological analyses of the burial pit content and encasing archaeological layers indicate that the pit was deliberately excavated. Taphonomical evidence, such as the strict articulation or good anatomical association of the skeletal elements and histological evidence of putrefaction, support the in-place decomposition of the fresh body. The presence of little or no displacement of the unstable joints during decomposition points to an interment in a filled space (grave earth), making the PYS finding the oldest known human burial in Africa. The morphological assessment of the partial skeleton is consistent with its assignment to Homo sapiens, although the preservation of some primitive features in the dentition supports increasing evidence for non-gradual assembly of modern traits during the emergence of our species. The PYS burial sheds light on how MSA populations interacted with the dead.Additional co-authors: Pilar Fernández-Colón, Nikos Kourampas, Jorge González García, David Larreina, François-Xavier Le Bourdonnec, Laura Martín-Francés, Diyendo Massilani, Julio Mercader, Jennifer M. Miller, Emmanuel Ndiema, Belén Notario, Africa Pitarch Martí, Mary E. Prendergast, Alain Queffelec, Solange Rigaud, Patrick Roberts, Mohammad Javad Shoaee, Ceri Shipton, Ian Simpson, Nicole Boivin & Michael D. Petragl

Characterization of Xenopus Tissue Inhibitor of Metalloproteinases-2: A Role in Regulating Matrix Metalloproteinase Activity during Development

Frog metamorphosis is totally dependent on thyroid hormone (T3) and mimics the postembryonic period around birth in mammals. It is an excellent model to study the molecular basis of postembryonic development in vertebrate. We and others have shown that many, if not all, matrix metalloproteinases (MMPs), which cleave proteins of the extracellular matrix as well as other substrates, are induced by T3 and important for metamorphosis. MMP activity can be inhibited by tissue inhibitors of metalloproteinase (TIMPs). There are 4 TIMPs in vertebrates and their roles in postembryonic development are poorly studied.We analyzed the TIMP2 genes in Xenopus laevis and the highly related species Xenopus tropicalis and discovered that TIMP2 is a single copy gene in Xenopus tropicalis as in mammals but is duplicated in Xenopus laevis. Furthermore, the TIMP2 locus in Xenopus tropicalis genome is different from that in human, suggesting an evolutionary reorganization of the locus. More importantly, we found that the duplicated TIMP2 genes were similarly regulated in the developing limb, remodeling intestine, resorbing tail during metamorphosis. Unexpectedly, like its MMP target genes, the TIMP2 genes were upregulated by T3 during both natural and T3-induced metamorphosis.Our results indicate that TIMP2 is highly conserved among vertebrates and that the TIMP2 locus underwent a chromosomal reorganization during evolution. Furthermore, the unexpected upregulation of TIMP2 genes during metamorphosis suggests that proper balance of MMP activity is important for metamorphosis

Omecamtiv mecarbil in chronic heart failure with reduced ejection fraction, GALACTIC‐HF: baseline characteristics and comparison with contemporary clinical trials

Aims: The safety and efficacy of the novel selective cardiac myosin activator, omecamtiv mecarbil, in patients with heart failure with reduced ejection fraction (HFrEF) is tested in the Global Approach to Lowering Adverse Cardiac outcomes Through Improving Contractility in Heart Failure (GALACTIC‐HF) trial. Here we describe the baseline characteristics of participants in GALACTIC‐HF and how these compare with other contemporary trials. Methods and Results: Adults with established HFrEF, New York Heart Association functional class (NYHA) ≥ II, EF ≤35%, elevated natriuretic peptides and either current hospitalization for HF or history of hospitalization/ emergency department visit for HF within a year were randomized to either placebo or omecamtiv mecarbil (pharmacokinetic‐guided dosing: 25, 37.5 or 50 mg bid). 8256 patients [male (79%), non‐white (22%), mean age 65 years] were enrolled with a mean EF 27%, ischemic etiology in 54%, NYHA II 53% and III/IV 47%, and median NT‐proBNP 1971 pg/mL. HF therapies at baseline were among the most effectively employed in contemporary HF trials. GALACTIC‐HF randomized patients representative of recent HF registries and trials with substantial numbers of patients also having characteristics understudied in previous trials including more from North America (n = 1386), enrolled as inpatients (n = 2084), systolic blood pressure < 100 mmHg (n = 1127), estimated glomerular filtration rate < 30 mL/min/1.73 m2 (n = 528), and treated with sacubitril‐valsartan at baseline (n = 1594). Conclusions: GALACTIC‐HF enrolled a well‐treated, high‐risk population from both inpatient and outpatient settings, which will provide a definitive evaluation of the efficacy and safety of this novel therapy, as well as informing its potential future implementation