Integrative analysis of metabolome and genome-wide transcriptome reveal the flavor changes in apple (Malus pumila Mill) after the novel acaricide cyflumetofen application

Pesticide residues were found to interfere the nutrition and flavor of fruits. Apple flavor changes after pesticides application was investigated based on metabolic dynamics and underlying regulatory networks. In this study, cyflumetofen (CYF) systematically affected the nutrition and flavor formation on apple (Malus pumila Mill). CYF alters nutritional composition, but not total content of soluble sugars and organic acids. Palatability-related amino acid content decreased around 15% in CYF-treated apple. The contents of esters and alcohols responsible for fragrance and flavor decreased by approximately 10% in CYF-treated apples compared with controls. Non-target metabolomic and transcriptomic analysis showed that CYF mainly affected amino acid-, organic acid-, polyphenol-, and lipid-metabolism related pathways, leading to altered nutritional and flavor characteristics. In conclusion, the results suggested that CYF affected the primary metabolism of apple, resulting in unpleasant changes in nutritional and flavor composition. This study provided new insight into the metabolic regulation of flavor after pesticides application.Universidade de Vigo/CISU

HPV infection and immunochemical detection of cell-cycle markers in verrucous carcinoma of the penis

Penile verrucous carcinoma is a rare disease and little is known of its aetiology or pathogenesis. In this study we examined cell-cycle proteins expression and correlation with human papillomavirus infection in a series of 15 pure penile verrucous carcinomas from a single centre. Of 148 penile tumours, 15 (10%) were diagnosed as pure verrucous carcinomas. The expression of the cell-cycle-associated proteins p53, p21, RB, p16INK4A and Ki67 were examined by immunohistochemistry. Human papillomavirus infection was determined by polymerase chain reaction to identify a wide range of virus types. The expression of p16INK4A and Ki67 was significantly lower in verrucous carcinoma than in usual type squamous cell carcinoma, whereas the expression of p53, p21 and RB was not significantly different. p53 showed basal expression in contrast to usual type squamous cell carcinoma. Human papillomavirus infection was present in only 3 out of 13 verrucous carcinomas. Unique low-risk, high-risk and mixed viral infections were observed in each of the three cases. In conclusion, lower levels of p16INK4A and Ki67 expressions differentiate penile verrucous carcinoma from usual type squamous cell carcinoma. The low Ki67 index reflects the slow-growing nature of verrucous tumours. The low level of p16INK4A expression and human papillomavirus detection suggests that penile verrucous carcinoma pathogenesis is unrelated to human papillomavirus infection and the oncogenes and tumour suppressor genes classically altered by virus infection.Peer reviewedFinal Accepted Versio

Evolutionarily diverse origins of deformed wing viruses in western honey bees

Novel transmission routes can allow infectious diseases to spread, often with devastating consequences. Ectoparasitic varroa mites vector a diversity of RNA viruses, having switched hosts from the eastern to western honey bees (Apis cerana to Apis mellifera). They provide an opportunity to explore how novel transmission routes shape disease epidemiology. As the principal driver of the spread of deformed wing viruses (mainly DWV-A and DWV-B), varroa infestation has also driven global honey bee health declines. The more virulent DWV-B strain has been replacing the original DWV-A strain in many regions over the past two decades. Yet, how these viruses originated and spread remains poorly understood. Here, we use a phylogeographic analysis based on whole-genome data to reconstruct the origins and demography of DWV spread. We found that, rather than reemerging in western honey bees after varroa switched hosts, as suggested by previous work, DWV-A most likely originated in East Asia and spread in the mid-20th century. It also showed a massive population size expansion following the varroa host switch. By contrast, DWV-B was most likely acquired more recently from a source outside East Asia and appears absent from the original varroa host. These results highlight the dynamic nature of viral adaptation, whereby a vector's host switch can give rise to competing and increasingly virulent disease pandemics. The evolutionary novelty and rapid global spread of these host-virus interactions, together with observed spillover into other species, illustrate how increasing globalization poses urgent threats to biodiversity and food security

Patient specific instrumentation allow precise derotational correction of femoral and tibial torsional deformities

Background: Rotational malalignment deformities of the lower limb in adults mostly arise from excessive femoral anteversion and/or excessive external tibial torsion. The aim of this study was to assess the correction accuracy of a patient specific cutting guides (PSCG) used in tibial and femoral correction for lower-limb torsional deformities.Methods: Forty knees (32 patients) were included prospectively. All patients had patellofe-moral pain or instability with torsional malalignment for which a proximal tibial (HTO) or distal femoral (DFO) or a double-level osteotomy (DLO) had been performed. Accuracy of the correction between the planned and the postoperative angular values including femoral anteversion, tibial torsion, coronal and sagittal alignment were assessed after tibial and/or femoral osteotomy.Results: Forty knees were included in this study. In cases of HTO, the correction accuracy obtained with PSCG was 1.3 +/- 1.1 degrees for tibial torsion (axial plane), 0.8 +/- 0.7 degrees for MPTA (coro-nal plane) and 0.8 +/- 0.6 degrees for PPTA (sagittal plane). In cases of DFO, the correction accuracy obtained with PSCG was 1.5 +/- 1.4 degrees for femoral anteversion (axial plane), 0.9 +/- 0.9 degrees for LDFA (coronal plane) and 0.9 +/- 0.9 degrees for PDFA (sagittal plane). The IKSG was improved from 58.0 +/- 13.2 degrees to 71.4 +/- 10.9 (p = 0.04) and the IKSF from 50.2 +/- 14.3 to 87.0 +/- 6.9 (p < 0.001).Conclusions: Using the PSCG for derotational osteotomy allows excellent correction accu-racy in all the three planes for femoral and tibial torsional deformities associated with patellofemoral instability.Level of clinical evidence II, prospective cohort study.(c) 2022 Elsevier B.V. All rights reserved

Patient specific instrumentation allow precise derotational correction of femoral and tibial torsional deformities

Background: Rotational malalignment deformities of the lower limb in adults mostly arise from excessive femoral anteversion and/or excessive external tibial torsion. The aim of this study was to assess the correction accuracy of a patient specific cutting guides (PSCG) used in tibial and femoral correction for lower-limb torsional deformities.Methods: Forty knees (32 patients) were included prospectively. All patients had patellofe-moral pain or instability with torsional malalignment for which a proximal tibial (HTO) or distal femoral (DFO) or a double-level osteotomy (DLO) had been performed. Accuracy of the correction between the planned and the postoperative angular values including femoral anteversion, tibial torsion, coronal and sagittal alignment were assessed after tibial and/or femoral osteotomy.Results: Forty knees were included in this study. In cases of HTO, the correction accuracy obtained with PSCG was 1.3 +/- 1.1 degrees for tibial torsion (axial plane), 0.8 +/- 0.7 degrees for MPTA (coro-nal plane) and 0.8 +/- 0.6 degrees for PPTA (sagittal plane). In cases of DFO, the correction accuracy obtained with PSCG was 1.5 +/- 1.4 degrees for femoral anteversion (axial plane), 0.9 +/- 0.9 degrees for LDFA (coronal plane) and 0.9 +/- 0.9 degrees for PDFA (sagittal plane). The IKSG was improved from 58.0 +/- 13.2 degrees to 71.4 +/- 10.9 (p = 0.04) and the IKSF from 50.2 +/- 14.3 to 87.0 +/- 6.9 (p < 0.001).Conclusions: Using the PSCG for derotational osteotomy allows excellent correction accu-racy in all the three planes for femoral and tibial torsional deformities associated with patellofemoral instability.Level of clinical evidence II, prospective cohort study.(c) 2022 Elsevier B.V. All rights reserved

Comparative mapping

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Pea (Pisum sativum L.) in the Genomic Era

Pea (Pisum sativum L.) was the original model organism used in Mendel’s discovery (1866) of the laws of inheritance, making it the foundation of modern plant genetics. However, subsequent progress in pea genomics has lagged behind many other plant species. Although the size and repetitive nature of the pea genome has so far restricted its sequencing, comprehensive genomic and post genomic resources already exist. These include BAC libraries, several types of molecular marker sets, both transcriptome and proteome datasets and mutant populations for reverse genetics. The availability of the full genome sequences of three legume species has offered significant opportunities for genome wide comparison revealing synteny and co-linearity to pea. A combination of a candidate gene and colinearity approach has successfully led to the identification of genes underlying agronomically important traits including virus resistances and plant architecture. Some of this knowledge has already been applied to marker assisted selection (MAS) programs, increasing precision and shortening the breeding cycle. Yet, complete translation of marker discovery to pea breeding is still to be achieved. Molecular analysis of pea collections has shown that although substantial variation is present within the cultivated genepool, wild material offers the possibility to incorporate novel traits that may have been inadvertently eliminated. Association mapping analysis of diverse pea germplasm promises to identify genetic variation related to desirable agronomic traits, which are historically difficult to breed for in a traditional manner. The availability of high throughput ‘omics’ methodologies offers great promise for the development of novel, highly accurate selective breeding tools for improved pea genotypes that are sustainable under current and future climates and farming systems.P.S. acknowledges financial support from the Ministry of Education of Czech Republic ME1006, ME10062, LA08011 and the Bioversity International AEGIS LOA 10/048 projects. J.M. acknowledges financial support from the Academy of Sciences of the Czech Republic (AVOZ50510513). T.D.W. acknowledges financial support from the Saskatchewan Pulse Growers and the Saskatchewan Ministry of Agriculture. C.J.C. acknowledges financial support from the United States Department of Agricultural, Agricultural Research Service