Cu(II) biosorption by living biofilms:Isothermal, chemical, physical and biological evaluation

Dissolved copper in stormwater runoff is a significant environmental problem. Biosorption of dissolved metals using microorganisms is known as a green, low-cost and efficient method. However, the role of live biological agents in the remediation of dissolved copper in Sustainable Drainage (SuDS) has not been reported. In this study, the effect of pH, initial concentration and temperature, on bacteria in different stages of biofilm development on a geotextile, along with Cu(II) removal efficiencies, were evaluated. Maximum Cu(II) removal efficiency (92%) was observed at pH 6. By decreasing the pH from 6 to 2, a log 5 reduction in bacteria was observed and Carboxyl groups transformed from -COO- to –COOH. The maximum biosorption capacity (119 mg g−1) was detected on day 1 of biofilm development, however, maximum removal efficiency (97%) was measured on day 21 of biofilm incubation. Extracellular Polymeric Substance (EPS) showed a better protection of CFUs in more mature biofilms (day 21) with less than 0.1 log decrease when exposed to 200 mL−1 Cu(II), whereas, biofilm on day 1 of incubation showed a 2 log reduction in CFUs number. Thermodynamic studies showed that the maximum Cu(II) biosorption capacity of biofilms, incubated for 7 days (117 mg g−1) occurred at 35 °C. Thermodynamic and kinetic modelling of data revealed that a physical, feasible, spontaneous and exothermic process controlled the biosorption, with a diffusion process observed in external layers of the biofilm, fitting a pseudo-second order model. Equilibrium data modelling and high R2 values of Langmuir model indicated that the biosorption took place by a monolayer on the living biofilm surface in all stages of biofilm development

Urban Gardens’ Potential to Improve Stormwater Management:A Comparative Analysis among Urban Soils in Sorocaba, São Paulo, Brazil

Permeable surfaces are increasingly rare in urban centers, but they have the utmost importance for stormwater infiltration. In this context, green spaces are key to reducing problems caused by runoff. This work aimed to evaluate the physical characteristics of the soil used for agroecological gardening, in comparison with parks, wasteland, and riparian forest in Sorocaba, São Paulo, Brazil. During the one-year data collection, urban gardens were superior to other areas in hydraulic conductivity (35.8 mm h−1), humidity (25.8%), and soil penetration resistance (1.21 MPa). On the other hand, the riparian forest showed signs of soil degradation, with low water infiltration rates (121.9 mm h−1) and humidity (14.4%). These findings highlight the importance of better soil management solutions to avoid compaction, such as the protection and conservation of riparian forests. Furthermore, the encouragement of urban gardens and parks with multiple uses can be an option for the enhancement of stormwater management in cities, since this practice has the potential to improve the physical characteristics of urban soils and provide several ecosystem services

Investigation of impact of slow-release fertilizer and struvite on biodegradation rate of diesel-contaminated soils

Fossil fuel remains the most used form of energy in the world and while it is only available in some countries, there is always the need to transport it to disadvantaged countries, which often results in oil spillages. Distribution of petroleum and petroleum products upstream and downstream also results in oil spillages. In cases where there is ineffective or no containment during and after oil spillages, the problem of oil pollution could be worsened by migration and seepage into receiving systems. In such a case, there is a need to identify and apply appropriate remediation techniques to reduce the impact and extent of the spillage. Some microbes have been identified as having the ability to degrade hydrocarbons and could potentially be harnessed for oil spillage clean-up but, would require sustainable nutrients supply to enhance biodegradation rate and efficiency. This study investigated the impact of slow-release fertilizer and struvite on the biodegradation rate and efficiency of diesel-contaminated soils with a view to determining whether struvite could be applied in the biodegradation of oil-contaminated soils as an alternative, low-cost, and sustainable approach. The test models were dosed with a cumulative oil loading of 178 mg m−2 week−1 based on the derived oil loading of 9.27 g m−2 year−1 with a view to comparing the efficiency of the nutrient sources. The nutrient sources, Osmocote Plus controlled-release fertilizer granules and struvite were applied at a one-off rate of 17 g m−2 to provide nutrient requirements and enhance biostimulation. The effect of the nutrients was studied by monitoring microbial growth in different growth media, the evolution of Carbon dioxide, elemental content by Inductively Coupled Plasma Atomic Emission Spectrometry, Electrical conductivity of effluent, Total Petroleum Hydrocarbons by infra-red spectroscopy, and pH of effluent. The results showed that Osmocote® plus controlled-release fertilizer is more effective in enhancing microbial growth than Struvite slow-release fertilizer hence, a relatively faster bioremediation rate. It should be noted that struvite accumulation in public water pipe works is an issue of environmental concern hence, any meaningful utilization and application of this “waste” in the bioremediation of contaminated soils would offer a sustainable and low-cost means of disposal of what is hitherto an environmental problem. However, it may require augmenting of the deficient nutrients to enhance effectiveness. This could be accomplished by incorporating naturally occurring nutrient sources such as rock phosphate, kelp, and bedrock nitrogen for sustainability