1 research outputs found
Nontargeted LC-MS<sup><i>n</i></sup> Profiling of Compounds in Ileal Fluids That Decrease after Raspberry Intake Identifies Consistent Alterations in Bile Acid Composition
Ileostomy studies provide a unique
insight into the digestion of foods, allowing identification of physiologically
relevant dietary phytochemicals and their metabolites that are important
to gut health. We previously reported an increase of components, including
novel triterpenoids, in ileal fluids of 11 ileostomates following
consumption of raspberries using nontargeted LC-MS<sup><i>n</i></sup> techniques in combination with data deconvolution software.
The current study focused on components that consistently decreased
postsupplementation. After data deconvolution, 32 components were
identified that met exclusion parameters of <i>m</i>/<i>z</i> signals and which decreased significantly in ileal fluids
from eight of 11 participants post-raspberry supplementation. Two-thirds
of these components were identified putatively from their MS properties.
Consistent decreases were observed in components that possibly reflected
“washing out” of presupplementation intake of common
foods/drinks including (poly)Âphenol metabolites. Metabolites associated
with fat metabolism such as hydroxylated fatty acids and cholate-type
bile acids were specifically reduced. However, more directed re-examination
of the data revealed that although some cholates were consistently
reduced, the more polar glyco- and tauro-linked bile acid derivatives
increased consistently, by as much as 100-fold over presupplementation
levels. The possible reasons for these substantial alterations in
bile acid composition in ileal fluids in response to raspberry intake
are discussed