Including Students with Disabilities and Achieving Accountability: Educators’ Emerging Challenge

The nation-wide movement toward increased accountability in our schools has been implemented in large part through state-mandated standardized testing of students. The state assessments in Texas, as in many states, have a powerful influence on educators’ decisions and practices. Whether or not students with special learning needs are provided with an educational experience in the least restrictive environment is dependent upon the decisions and actions of educators. The high-stakes testing agenda in Texas influences decisions related to the inclusion of students with special learning needs. In this article, we examine the issue of how the inclusion of students with disabilities is being achieved in the midst of the high-stakes testing environment of south Texas. Using a questionnaire mailed to general and special education teachers along with school administrators, we examine educators’ attitudes/practices concerning inclusion and testing

miRNAs and their role in neural crest development

The neural crest (NC) is a multipotent, migratory cell population that is unique to vertebrate embryos and gives rise to many derivatives, such as the craniofacial skeleton, sensory neurons and pigment cells. A complex gene regulatory network underlies the process of NC formation, which involves the early induction of the neural plate border (NPB), specification of the NC, migration of the NC away from the neural tube along distinct pathways and differentiation into diverse cell types. microRNAs (miRNAs) are a class of non-coding regulatory genes, which act posttranscriptionally to regulate gene expression. They are of widespread significance and have been implicated in many biological processes. Many miRNAs have now been identified, however, as of yet, they have not been shown to have any direct roles in early NC development. Using various molecular techniques this study has placed specific miRNAs within the complex NC gene network. These miRNAs are miR-196a and miR-219. SRNA sequencing of induced Xenopus NC tissue generated a miRNA expression profile which in combination with whole mount in situ hybridisation (WISH) revealed multiple candidate miRNAs expressed in NC. Using knockdown (KD) experiments, the depletion of miR-196a and miR-219 resulted in aberrant NC development including abnormal craniofacial cartilage development. Using luciferase assays, this study shows for the first time that miR-219 directly targets the transcription factor Eya1 in vitro. This gene lies directly upstream of the NPB marker Pax3. When miR-219 is knocked down, Pax3 expression is expanded across the surface ectoderm of the embryo suggesting the miR-219 serves to inhibit the Pax3 domain. To begin to understand the molecular mechanisms behind both this phenotype and why the NC is lost, RNA sequencing on dissected NC tissue was employed. Results from this sequencing data demonstrated that following miR-219 KD the NPB and the placodes form whilst the NC is lost. This indicates that miR-219 is playing a role in ensuring the correct specification of NC. In comparison, following miR-196a KD the NPB development is impaired and derivatives are lost (placode and NC). This implies miR-196a has an earlier role in ensuring the correct induction of the NPB possibly through fine-tuning early inducing signals such as BMP and Notch. Using the data presented in this study, the first models of how specific miRNAs could function in NC development have been formulated

Food Policy Council Self-Assessment Tool: Development, Testing, and Results

A large number of food policy councils (FPCs) exist in the United States, Canada, and Tribal Nations (N = 278), yet there are no tools designed to measure their members' perceptions of organizational capacity, social capital, and council effectiveness. Without such tools, it is challenging to determine best practices for FPCs and to measure change within and across councils over time. This study describes the development, testing, and findings from the Food Policy Council Self-Assessment Tool (FPC-SAT). The assessment measures council practices and council members' perceptions of the following concepts: leadership, breadth of active membership, council climate, formality of council structure, knowledge sharing, relationships, member empowerment, community context, synergy, and impacts on the food system. All 278 FPCs listed on the Food Policy Network's Online Directory were recruited to complete the FPC-SAT. Internal reliability (Cronbach's α) and inter-rater reliability (AD, rWG(J), ICC [intraclass correlations][1], ICC[2]) were calculated, and exploratory and a confirmatory factor analyses were conducted. Responses from 354 FPC members from 94 councils were used to test the assessment. Cronbach's α ranged from 0.79 to 0.93 for the scales. FPC members reported the lowest mean scores on the breadth of active membership scale (2.49; standard deviation [SD], 0.62), indicating room for improvement, and highest on the leadership scale (3.45; SD, 0.45). The valid FPC-SAT can be used to identify FPC strengths and areas for improvement, measure differences across FPCs, and measure change in FPCs over time

A Practical Guide for Managing Interdisciplinary Teams: Lessons Learned from Coupled Natural and Human Systems Research

Interdisciplinary team science is essential to address complex socio-environmental questions, but it also presents unique challenges. The scientific literature identifies best practices for high-level processes in team science, e.g., leadership and team building, but provides less guidance about practical, day-to-day strategies to support teamwork, e.g., translating jargon across disciplines, sharing and transforming data, and coordinating diverse and geographically distributed researchers. This article offers a case study of an interdisciplinary socio-environmental research project to derive insight to support team science implementation. We evaluate the project’s inner workings using a framework derived from the growing body of literature for team science best practices, and derive insights into how best to apply team science principles to interdisciplinary research. We find that two of the most useful areas for proactive planning and coordinated leadership are data management and co-authorship. By providing guidance for project implementation focused on these areas, we contribute a pragmatic, detail-oriented perspective on team science in an effort to support similar projects

Antagonistic regulation of mRNA expression and splicing by CELF and MBNL proteins

RNA binding proteins of the conserved CUGBP1, Elav-like factor (CELF) family contribute to heart and skeletal muscle development and are implicated in myotonic dystrophy (DM). To understand their genome-wide functions, we analyzed the transcriptome dynamics following induction of CELF1 or CELF2 in adult mouse heart and of CELF1 in muscle by RNA-seq, complemented by crosslinking/immunoprecipitation-sequencing (CLIP-seq) analysis of mouse cells and tissues to distinguish direct from indirect regulatory targets. We identified hundreds of mRNAs bound in their 3′ UTRs by both CELF1 and the developmentally induced MBNL1 protein, a threefold greater overlap in target messages than expected, including messages involved in development and cell differentiation. The extent of 3′ UTR binding by CELF1 and MBNL1 predicted the degree of mRNA repression or stabilization, respectively, following CELF1 induction. However, CELF1's RNA binding specificity in vitro was not detectably altered by coincubation with recombinant MBNL1. These findings support a model in which CELF and MBNL proteins bind independently to mRNAs but functionally compete to specify down-regulation or localization/stabilization, respectively, of hundreds of mRNA targets. Expression of many alternative 3′ UTR isoforms was altered following CELF1 induction, with 3′ UTR binding associated with down-regulation of isoforms and genes. The splicing of hundreds of alternative exons was oppositely regulated by these proteins, confirming an additional layer of regulatory antagonism previously observed in a handful of cases. The regulatory relationships between CELFs and MBNLs in control of both mRNA abundance and splicing appear to have evolved to enhance developmental transitions in major classes of heart and muscle genes.Myotonic Dystrophy FoundationNational Institutes of Health (U.S.) (Grant OD017865-02)National Science Foundation (U.S.) (Award 0821391)National Institutes of Health (U.S.) (Grant R01GM085319)National Institutes of Health (U.S.) (Grant RC2HG005624

A nervous disorder in cattle, caused by the plants Ficus ingens var. ingens and Ficus cordata subsp. salicifolia

Two outbreaks of neurotoxicoses are reported in cattle browsing on the leaves of Ficus spp. In the first outbreak, three animals died and one became ill. A sheep developed severe nervous signs, including tetanic spasms, when dosed with the leaves of Ficus ingens var. ingens from the toxic camp where the cattle had died. The second outbreak resulted in the death of 12 heifers within 48 h of ingestion of the leaves of F. cordata subsp. salicifolia. Clinical signs included hyperaesthesia, ataxia, muscle tremors and paddling motions while in lateral recumbency. Similar signs were reproduced by drenching the incriminated leaves to a steer. The sheep dosed with F. ingens var. ingens and two cattle, one of which had died during the second outbreak and the steer drenched with F. cordata subsp. salicifolia, were necropsied. Light microscopical examination consistently revealed oedema of the central nervous system. In the steer, focal demyelination was evident in localized areas of the brain and spinal cord. Liver lesions ranged from mild degeneration to focal disseminated necrosis of hepatocytes.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

A database of microRNA expression patterns in Xenopus laevis

MicroRNAs (miRNAs) are short, non-coding RNAs around 22 nucleotides long. They inhibit gene expression either by translational repression or by causing the degradation of the mRNAs they bind to. Many are highly conserved amongst diverse organisms and have restricted spatio-temporal expression patterns during embryonic development where they are thought to be involved in generating accuracy of developmental timing and in supporting cell fate decisions and tissue identity. We determined the expression patterns of 180 miRNAs in Xenopus laevis embryos using LNA oligonucleotides. In addition we carried out small RNA-seq on different stages of early Xenopus development, identified 44 miRNAs belonging to 29 new families and characterized the expression of 5 of these. Our analyses identified miRNA expression in many organs of the developing embryo. In particular a large number were expressed in neural tissue and in the somites. Surprisingly none of the miRNAs we have looked at show expression in the heart. Our results have been made freely available as a resource in both XenMARK and Xenbase

Geological Field Trips

This field trip guide organized in the framework of the Goldschmidt Conference 2013, held in Florence from August 25 to 30, 2013, is here presented. The two-days field trip, shows some of the many geological, naturalistic and cultural features in the Fiorano area (Modena), in which history, geology and passion for Ferrari come together in a perfect marriage. The first excursion day is dedicated to visit the Natural Reserve of Salse di Nirano, where the mud volcanoes, produced by the cold mud, salt water and hydrocarbons - mainly methane- can be observed. The second day is devoted to visit the Ferrari Museum and goes on at the Spezzano Castle, hosting the Ceramics Museum. Clays are, in fact, abundant in the hilly margin, where they form badlands, characteristic narrow crests washed out by running waters. In the Castle there is also a Balsamic Vinegar producing Consortium, it’s a peculiar and typical product of Modena province. The itinerary ends with the tour to Enzo Ferrari’s Birthplace at Modena

An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus

In recent years CRISPR-Cas9 knockouts (KO) have become increasingly ultilised to study gene function. MicroRNAs (miRNAs) are short non-coding RNAs, 20–22 nucleotides long, which affect gene expression through post-transcriptional repression. We previously identified miRNAs-196a and −219 as implicated in the development of Xenopus neural crest (NC). The NC is a multipotent stem-cell population, specified during early neurulation. Following EMT, NC cells migrate to various points in the developing embryo where they give rise to a number of tissues including parts of the peripheral nervous system, pigment cells and craniofacial skeleton. Dysregulation of NC development results in many diseases grouped under the term neurocristopathies. As miRNAs are so small, it is difficult to design CRISPR sgRNAs that reproducibly lead to a KO. We have therefore designed a novel approach using two guide RNAs to effectively ‘drop out’ a miRNA. We have knocked out miR-196a and miR-219 and compared the results to morpholino knockdowns (KD) of the same miRNAs. Validation of efficient CRISPR miRNA KO and phenotype analysis included use of whole-mount in situ hybridization of key NC and neural plate border markers such as Pax3, Xhe2, Sox10 and Snail2, q-RT-PCR and Sanger sequencing. To show specificity we have also rescued the knockout phenotype using miRNA mimics. miRNA-219 and miR-196a KO’s both show loss of NC, altered neural plate and hatching gland phenotypes. Tadpoles show gross craniofacial and pigment phenotypes

Micrometeoroid Events in LISA Pathfinder

The zodiacal dust complex, a population of dust and small particles that pervades the Solar System, provides important insight into the formation and dynamics of planets, comets, asteroids, and other bodies. Here we present a new set of data obtained using a novel technique: direct measurements of momentum transfer to a spacecraft from individual particle impacts. This technique is made possible by the extreme precision of the instruments flown on the LISA Pathfinder spacecraft, a technology demonstrator for a future space-based gravitational wave observatory that operated near the first Sun-Earth Lagrange point from early 2016 through Summer of 2017. Using a simple model of the impacts and knowledge of the control system, we show that it is possible to detect impacts and measure properties such as the transferred momentum (related to the particle's mass and velocity), direction of travel, and location of impact on the spacecraft. In this paper, we present the results of a systematic search for impacts during 4348 hours of Pathfinder data. We report a total of 54 candidates with momenta ranging from 0.2$\,\mu\textrm{Ns}$ to 230$\,\mu\textrm{Ns}$. We furthermore make a comparison of these candidates with models of micrometeoroid populations in the inner solar system including those resulting from Jupiter-family comets, Oort-cloud comets, Hailey-type comets, and Asteroids. We find that our measured population is consistent with a population dominated by Jupiter-family comets with some evidence for a smaller contribution from Hailey-type comets. This is in agreement with consensus models of the zodiacal dust complex in the momentum range sampled by LISA Pathfinder.Comment: 22 pages, 14 figures, accepted in Ap