qPCR validation of RNA-seq result.

<p>Quantitative PCR for (A) <i>gli1</i>, (B) <i>ptch1</i>, and (C) <i>hsd11b1</i> was performed on Hh ligand treated and Hedgehog ligand and SFE co-treated TRAMPC2 cells. Transcripts concentrations were normalized to control. * indicates p<0.05. In the lower figure, transcripts concentrations of (A) <i>gli1</i>, (B) <i>ptch1</i>, and (C) <i>hsd11b1</i> are represented by quantified sequencing reads, in the form of counts-per-million-reads.</p

Sutherlandia Extract alters genes in TRAMPC2 cells.

<p>(A) Differentially expressed genes in response to Sutherlandia extract treatment. Genes that are related to (B, C, D) are labeled. (B) Gene Ontology analysis of Sutherlandia responsive genes. (C, D) KEGG pathway analysis of Sutherlandia responsive genes.</p

Heat map of Sutherlandia Extract altered Hedgehog-signaling pathway target genes expression.

<p>TRAMPC2 cell were treated with either Hh-CM or co-treated with Hh-CM and 80μg/ml SFE. Over 50% of Hh-responsive genes were repressed by SFE treatment. Gene expression values were represented by Log2 transformed normalized RNA-seq reads (Log2 count-per-million-reads) and color coded.</p