Antifungal activity and acute toxicity of stem bark extracts of Drypetes Gossweileri S. Moore-Euphorbiaceae from Cameroon

Drypetes gossweilleri S. Moore is a plant used in traditional medicine in Cameroon. The antifungal properties of its stem-bark crude extract and fractions DG1, DG2, DG3, DG4, DG5, DG6, DG7, DG8 and DG9 were assayed by agar and broth dilution methods on solid and liquid media against C. Krusei, C. albicans, C. glabrata, T. mentagerophytes, M. langeroinii, M. gypeum, M. audouini, T. rubrum, T. soudanense, T. terrestre, A. flavus and A. niger. The results revealed a substantial antifungal effect with minimal inhibitory concentrations ranging respectively from 24.11μg/ml to 1562μg/ml for yeasts and from 3125μg/ml to 12500μg/ml for filamentous fungi. Among the fractions, fraction DG4 exerted the highest antifungal activity. Moreover, no toxic effect was noticed in male and female albinos Wistar rats treated per os with the crude stem bark’s extract of Drypetes gossweileri at a dose up to 12g/kg of body weight. The phytochemical screening of the crude extract and fractions showed the presence of alkaloids, phenols, flavonoids, saponins, anthocyanines, anthraquinones, sterols, lipids and essential oils. Therefore, Drypetes gossweileri may be safe as phytomedecine for the treatment of fungal infections.Key words: Antifungal activity, Drypetes gossweileri, acute toxicity

Structural Arrangements of Isomorphic Substitutions in Smectites: Molecular Simulation of the Swelling Properties, Inter layer Structure, and Dynamics of Hydrated Cs-Montmorillonite Revisited with New Clay Models

International audienceThree new structural models of montmorillonite with differently distributed Al/Si and Mg/Al substitutions in the tetrahedral and octahedral clay layers are systematically developed and studied by means of MD simulations to quantify the possible effects of such substitutional disorder on the swelling behavior, the interlayer structure, and mobility of aqueous species. A very wide range of water content, from 0 to 700 mg(water)/g(clay) is explored to derive the swelling properties of Cs-montmorillonite. The determined layer spacing does not differ much depending on the clay model. However, at low water contents up to 1-layer hydrate (similar to 100 mg(water)/g(clay)) the variation of specific locations of the tetrahedral and octahedral substitutions in the two TOT clay layers slightly but noticeably affects the total hydration energy of the system. Using atom-atom radial distribution functions and the respective atomic coordination numbers we have identified for the three clay models not only the previously observed binding sites for Cs+ on the clay surface but also new ones that are correlated with the position of tetrahedral substitution in the structure. The mobility of Cs+ ions and H2O diffusion coefficients, as expected, gradually increase both with increasing water content and with increasing distance from the clay surface, but they still remain 2 to 4 times lower than the corresponding bulk values. Only small differences were observed between the three Cs-montmorillonite models, but these differences are predicted to increase in the case of higher charge density of the clay layers and/or interlayer cations