An investigation into untreated greywater as supplementary household water source to augment potable municipal supply with consideration of associated risks.

Thesis (PhD)--Stellenbosch University, 2022.ENGLISH ABSTRACT: Despite the available body of research regarding supplementary household water sources and in particular, greywater use, there is a critical gap when it comes to understanding the uptake of untreated greywater in suburban areas and the trade-off between the risks and potential water savings. This dissertation focuses on untreated greywater use in residential, fully serviced houses equipped with regular water use appliances and with conventional waterborne sewers. The main objective is to gain an improved understanding of the uptake of untreated greywater and the potential for use and application in suburban areas by exploring the trade-off between expected water savings (associated with quantity) and potential risks (associated with quality) as related to untreated greywater use. This study starts with addressing on-site supplementary household water sources with a focus on groundwater abstraction, rainwater harvesting, and greywater use as available non-potable supplementary water sources to residential consumers. The legal position in South Africa and an end use model to assess the theoretical impact of these sources on water demand in formal residential areas, is presented. The model provides valuable strategic direction and indicates a significant theoretical reduction in potable municipal water demand of between 55% and 69% for relatively large properties with irrigated gardens when supplementary household sources are maximally utilised (when compared to exclusive municipal use as a baseline). This load reduction on piped reticulation systems could be an advantage through augmenting municipal supply. However, water service planning and demand management are complicated by the introduction, and possible future decommissioning, of any household water source. The trade off between the advantages and disadvantages of this load reduction defines whether there is a nett positive benefit linked to the use of the household water sources. Groundwater is the household water source considered to have the most notable penetration and intensity to impact potable water demand in residential areas and is coupled to a relatively low risk in terms of water quality relative to other uses such as greywater use. Groundwater, however, has the biggest barrier to entry and requires the highest capital investment of the three supplementary household water sources. The distinct trade off between the advantages and disadvantages of untreated greywater, particularly in comparison to the other supplementary household water sources, provides justification towards it being the focus of this study. Untreated greywater use at household level is an accessible water source to supplement non-potable water requirements in times of emergency water curtailments, but poses various risks to the consumer, the wider community, infrastructure and the environment. Little is known about unregulated, untreated greywater use practices in suburban communities where consumers have become accustomed to reliable potable water supplied via a pressurised, piped distribution system. There is a lack of knowledge regarding the sources of greywater used, collection methods, -storage and -distribution, the application points, the level of treatment (if any) and the perceived risks related to the greywater use. The City of Cape Town was selected as a case study site for research into greywater use under the threat of “Day Zero” and stringent water restrictions, implemented during the 2017/2018 summer season. A consumer survey and analysis of relevant online forums was conducted in order to obtain the necessary information. Greywater use practices from a sample group of 351 consumers were identified and classified. Untreated greywater use was found to be common, mainly for garden irrigation and toilet flushing. The results point to high-risk activities in the study group. By using these reported ad hoc greywater use practices identified through the Cape Town case study, the volume of untreated greywater used by households in formal residential settings was evaluated by means of a stochastic end use model. Untreated greywater use practices (e.g. bucketing) were found to reduce water consumption in a single person suburban household by less than 10%, which is lower than values reported in literature. This relatively low volume weighed up against the high risk of using untreated greywater may result in a negative nett benefit, providing decision making insights for both water service providers and consumers. This quantification of the volumes associated with untreated ad hoc greywater use is the first step in understanding the trade-off between expected water savings (quantity) and potential risks (quality) of untreated greywater use. The second component of the water saving-risk trade off involved an investigation of untreated greywater quality and related risks, through a statistical analysis of greywater quality results, as sourced from South African studies. Greywater sources included were the bathroom, kitchen, laundry, mixed and general residential sources. Variability in terms of each of the reported physical, chemical and microbiological constituents by source and between result sets was noted. Statistically significant differences were evident between the pH, conductivity and phosphorous values of certain sources. A risk assessment undertaken for each of the constituents revealed further variability. The constituent with the highest number of high-risk samples was total dissolved solids, although further research into specific constituent elements that are of real danger to humans is warranted here. The finding that water savings due to untreated greywater through manual collection methods is <10% is markedly less than the water savings through the use of multiple household water sources (up to 69% for large properties). This coupled with the relatively high risk and high consequences in greywater practices in terms of public health, the environment, and infrastructure, given its variability, provide insight into the quality-quantity space. There is a need for a more nuanced view of the potential potable savings associated with greywater use and a need for improved risk management. Risk management and drivers of consumer decision making in the water use space were therefore explored further. As a result, a decision-making matrix was designed as an interim conceptual tool to assist consumers when faced with water use decisions during emergency drought conditions. This research is unique in that while the use of greywater with purpose-built infrastructure and treatment systems has been studied for a number of locations and configurations, the practices used by individuals in the absence of such infrastructure was not well understood. This study has shed light on the reported volume of untreated greywater used by households in formal residential settings, based on reported ad hoc greywater use practices and on the extent of these potentially risky practices. A novel holistic picture of the risks and trade-offs associated with untreated greywater use was developed, allowing for advancement of knowledge in the field.AFRIKAANSE OPSOMMING: Geen opsomming beskikbaarDoctora

Baseline adjustment methodology in a shared water savings contract during severe water restrictions – a case study in the Western Cape, South Africa

A novel method for baseline adjustment in a shared water savings contract under serious drought conditions was presented in a companion paper. The newly developed baseline adjustment method was subsequently applied to a case study, as discussed in this manuscript. The case study involved application of the method to 24 relatively complex sites, spread over the Western Cape Province in South Africa. The sites included, for example, military bases, naval dockyards, an airforce base, prison facilities, large multi-storey blocks of flats and administrative office buildings. Baseline adjustment became essential mid-contract during the serious water restrictions in Cape Town at the time. The restrictions were linked to the ‘Day Zero’ scenario in 2018 when water supply would potentially run out, and resulted in water savings at baseline sites that were ascribed to external factors. The study incorporated a comprehensive review of the approved baseline reports with site visits to 12 of the properties. The baseline adjustment method provided a robust means to obtain adjustments for sites with relatively limited data. The minimum data requirement was a record of monthly water consumption per site. The adjustments varied between 0% and 64% of the original baseline value for the different sites in the study sample. The relatively higher adjustments were linked to sites where outdoor irrigation and pool water use was prevalent during the baseline-setting period, but was banned during the drought. Zero adjustments were found for sites with exceptionally high leakage flows that had subsequently been repaired; leaks dwarfed actual use in these cases. The results for all 24 sites were accepted by the contracting parties as being reasonable and fair.Keywords: baselines, shared water savings contrac

A field outbreak in Ile-de-France sheep of a cardiotoxicosis caused by the plant Pachystigma pygmaeum (Schltr) Robyns (Rubiaceae)

A field outbreak of Pachystigma pygmaeum intoxication in sheep is described. Noteworthy clinical signs were: respiratory distress, apathy and subcutaneous oedema of mainly the head. Gross changes included cardiomegaly, centrilobular hepatic necrosis and effusion of body cavities. Microscopically myocardial fibrosis, affecting predominantly the endocardium of the apex, left free ventricular wall and interventricular septum, was most striking in the majority of animals, whilst myofibre atrophy was present in 1 sheep.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

Concurrent babesiosis and ehrlichiosis in the dog : blood smear examination supplemented by the indirect fluorescent antibody test, using Cowdria ruminantium as antigen

Giemsa-stained, peripheral blood smears of 67 dogs, showing clinical signs typical of babesiosis or reminiscent of concurrent babesiosis and ehrlichiosis, were examined for the presence of Babesia canis and Ehrlichia canis. Since Cowdria ruminantium cross-reacts with Ehrlichia, the sera of these dogs were also subjected to the indirect fluorescent antibody (IFA) test in which C. ruminantium was used as antigen. Fifty-five per cent of these dogs had mixed infections of B. canis and E. canis, as judged by blood smear examination and serology. The serum of 32 % of these dogs with mixed infections reacted positively in the IFA test. Six out of 9 dogs, the blood smears of which were negative for both B. canis and E. canis, were serologically positive for E. canis. Furthermore, sero-conversion from a negative in the initial serum sample to titres of up to 1:160 in a subsequent sample was recorded in 9 out of 13 dogs with suspected mixed infection on blood smear.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Cardiomyopathy of ruminants induced by the litter of poultry fed on rations containing the ionophore antibiotic, maduramicin. 1. Epidemiology, clinical signs and clinical pathology

The epidemiological, clinical and clinical pathological findings in 20 cattle and 4 sheep from 15 outbreaks of poultry litter toxicity in South Africa over the past 6 years are documented. In 6 outbreaks, the litter emanated from batteries where maduramicin had been incorporated into rations of broilers. According to circumstantial evidence the litter involved in the 9 other outbreaks was also derived from broilers which had been fed on rations containing an ionophore. The litter was fed ad libitum to the affected stock or constituted 30-80% by volume of their rations. The principal sign manifested was sudden mortality of up to 70% of the herd or flock, usually within 20-40 days of commencement of feeding of poultry litter. A few cattle developed signs of congestive heart failure, and stiffness was commonly seen in sheep. In a dosing trial with poultry litter involving 1 steer and 6 sheep, the steer and a sheep died suddenly and a second sheep was destroyed in extremis. Tachycardia and/or cardiac arrythmia were recorded in 5 sheep, and the activity of aspartate transaminase (AST) and/or lactate dehydrogenase (LD) in the sera of 4 was elevated. Since the cardiac lesions in field cases were similar to those of ionophore poisoning and broiler rations containing maduramicin was a common factor in several outbreaks, toxic litter from some of these outbreaks were tested for the presence of this compound. Analysis by high performance liquid chromatography of litter from 2 specimens of outbreaks revealed that they contained 2,5ppm and 6,1ppm maduramicin. Sheep in a trial fed rations incorporating c 2,5ppm and 5ppm maduramicin, developed clinical signs and lesions consistent with those of the field cases.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Cardiomyopathy of ruminants induced by the litter of poultry fed on rations containing the ionophore antibiotic, maduramicin. II. Macropathology and histopathology

This report contains an account of the gross and histopathological lesions of 20 cattle and four sheep in 15 field outbreaks of poultry litter toxicity, one steer fed ad lib. and six sheep dosed with toxic poultry litter, and ten sheep fed experimental rations containing c 2,5 ppm and 5 ppm maduramicin. The principle macroscopic lesions in most cattle that died in field outbreaks were indicative of congestive heart failure. The lesions in sheep were similar, but generally milder. Cardiac dilatation was observed in both sheep and cattle. Microscopically, the cardiac lesions were more pronounced in cattle and comprised varying degrees of atrophy, hypertrophy, degeneration, necrosis of myocardial fibres, and interstitial fibrosis. Skeletal muscle lesions were usually more severe in sheep, particularly in the muscles of the hindquarters which appeared pale, oedematous and mottled. One of the sheep in the poultry litter dosing trial developed signs of congestive heart failure and the hearts of two others were dilated. Extensive hypertrophy and atrophy of myocardial fibres were evident in the steer fed ad lib. with this material. As in field cases, the myocardial lesions of the sheep were less severe than those of the steer. Mild cardiac dilatation was present in four of the seven sheep in the maduramicin feeding trial. Diffuse hypertrophy of myocardial nuclei was present in all seven cases, myocardial fibre atrophy in six, multifocal fibrosis and necrosis in six and two cases, respectively, and focal endocardial thickening in two. The skeletal muscles revealed granular degeneration and foci of necrosis and regeneration. The cardiac and skeletal lesions in the field outbreaks, poultry litter feeding trials and maduramicin feeding trials, were highly comparable. This suggests that this form of poultry litter intoxication is a chronic form of ionophore toxicity, the pathology of which is characterized by a dilated cardiomyopathy with congestive heart failure and mild (cattle) to severe (sheep) skeletal muscle lesions.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Baseline adjustment methodology in a shared water savings contract under serious drought conditions

CITATION: Jacobs, H. E. et al. 2020. Baseline adjustment methodology in a shared water savings contract under serious drought conditions. Water SA, 46(1):22-29, doi:10.17159/wsa/2020.v46.i1.7874.The original publication is available at: http://www.scielo.org.zaENGLISH ABSTRACT: Baselines are often employed in shared water saving contracts for estimating water savings after some type of intervention by the water service company. An adjustment to the baseline may become necessary under certain conditions. Earlier work has described a number of relatively complex methods for baseline determination and adjustment, but application in regions faced with relatively limited data becomes problematic. If the adjustment were determined before finalising the contractual matters, it would be possible to gather the required data in order to determine the adjustment. However, in cases where no adjustment was fixed prior to the contract, a method is required to determine an adjustment mid-contract based on whatever data are available at the time. This paper presents a methodology for baseline adjustment in an existing shared water savings contract and explains how adjustment could be determined mid-contract, under conditions of limited data. The adjustment compensates for expected reduced water consumption due to external influences induced by serious water restrictions, typically introduced during periods of drought. The fundamental principle underpinning the baseline adjustment methodology presented in this paper involved segregating real water losses from the actual consumption of end-users, preferably by analysing the minimum night flow. In the absence of recorded night flows, an alternative procedure involving the minimum monthly consumption pre- and post-baseline was employed. The baseline adjustment method was subsequently applied in a South African case study, reported on separately. This technique is helpful because adjustments could be determined without adding unnecessary complexity or cost, and provides a means to resolve disputes in cases where unexpected savings occur mid-contract.Publisher's versio

Baseline adjustment methodology in a shared water savings contract under serious drought conditions

CITATION: Jacobs, H. E. et al. 2020. Baseline adjustment methodology in a shared water savings contract under serious drought conditions. Water SA, 46(1):22-29, doi:10.17159/wsa/2020.v46.i1.7874.The original publication is available at: http://www.scielo.org.zaENGLISH ABSTRACT: Baselines are often employed in shared water saving contracts for estimating water savings after some type of intervention by the water service company. An adjustment to the baseline may become necessary under certain conditions. Earlier work has described a number of relatively complex methods for baseline determination and adjustment, but application in regions faced with relatively limited data becomes problematic. If the adjustment were determined before finalising the contractual matters, it would be possible to gather the required data in order to determine the adjustment. However, in cases where no adjustment was fixed prior to the contract, a method is required to determine an adjustment mid-contract based on whatever data are available at the time. This paper presents a methodology for baseline adjustment in an existing shared water savings contract and explains how adjustment could be determined mid-contract, under conditions of limited data. The adjustment compensates for expected reduced water consumption due to external influences induced by serious water restrictions, typically introduced during periods of drought. The fundamental principle underpinning the baseline adjustment methodology presented in this paper involved segregating real water losses from the actual consumption of end-users, preferably by analysing the minimum night flow. In the absence of recorded night flows, an alternative procedure involving the minimum monthly consumption pre- and post-baseline was employed. The baseline adjustment method was subsequently applied in a South African case study, reported on separately. This technique is helpful because adjustments could be determined without adding unnecessary complexity or cost, and provides a means to resolve disputes in cases where unexpected savings occur mid-contract.Publisher's versio

The Broad-Brush Survey Approach. A set of methods for rapid qualitative community assessment

Using a combination of qualitative data collection methods to collect data rapidly from a place on a particular topic is not a novel idea. Rapid participatory and qualitative appraisal approaches have been used in many different settings for the past 40 years, with the influential scholar Robert Chambers, and those he worked with, doing much to shape the practice from the 1970s. The methods spread beyond a rural, agriculture focus (Chambers, 1994) to embrace urban settings and the assessment of health and other areas of interest as well as settings in the Global North as well as South (Annett and Rifkin, 1995, Murray et al., 1994). I first used these approaches in the 1980s, while working in the Annapurna foothills in Nepal at an agricultural research station. We established the practice of a one week data collection exercise, which we called a `Combined Trek’ where a group of scientists from different disciplines, including me – a social anthropologist – systematically collected information using interviews, observations and discussions in a village and the surrounding area – working closely with the local people. Our purpose was to inform future agricultural interventions, building from what people were already doing. Cecilia Vindrola-Padros and Ginger Johnson (2020) detail in a review article how different qualitative methods have been adapted to be used to collect data rapidly. The need for speed, as they explain, has been a response to the increasing pressure many of us are under to deliver study findings quickly. Their review sets out how conventional methods have been adapted to be used rapidly in different settings. Among the combination of methods that they describe is the `rapid ethnographic assessment’. This assessment approach has grown as a response of anthropologists to pressure to produce results far more quickly that more conventional ethnographic approaches would allow. This set of methods is described in detail in the recent manual produced by Sangaramoorthy and Kroeger (2020). We are not, therefore, claiming that the approach set out in this manual is particularly novel nor indeed unique. The Broad Brush Survey, described in this manual is an approach originally developed by Valdo Pons (1993, 1996) and further developed and popularized through the work of Sandra Wallman (1996), which can be used to capture both the landscape and ‘feel’ of a community and the people in it. The research findings can be used to shape further investigators or interventions to address the problem at hand in a useful and practical manner rapidly, succinctly and systematically. This `Broad Brush Survey’ approach manual is, therefore, a contribution to the burgeoning literature on methods for rapid qualitative data collection methods and assessment. The use of the word ‘survey’ in the title of the set of methods may be perplexing to those who consider the term to be synonymous with `questionnaire’. This 6 is not the way we use the word – the Oxford English Dictionary offers several definitions of word `survey’, which include `the act of viewing, examining, or inspecting in detail [...] for some specific purpose’ and `the, or an, act of looking at something as a whole from a commanding position; a general or comprehensive look’. Both definitions convey the sense of our intention: to engage with, and in, a community for a short but concentrated period of time, seeking quickly, but thoroughly, to take a comprehensive look at the place for a specific purpose, and document the place at that moment in time. As we explain in the first chapter, the approach is systematic with a defined sequence of qualitative data collection methods, which gradually allows the user to build an understanding of place and people. The combination of methods used, however, is not set in stone and can be adapted to suit the purpose at hand. As such we hope that this manual serves as a guide to the possibilities which using this approach can offer both for those working in interdisciplinary projects as well as those from anthropology and sociology, for example, laying the groundwork for in-depth longitudinal research

Quality of Life in Men With Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency

Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (21OHD) is a disorder of adrenal steroid biosynthesis, leading to hypocortisolism, hypoaldosteronism, and hyperandrogenism. Impaired quality of life (QoL) has been demonstrated in women with CAH, but data on men with CAH are scarce. We hypothesized that disease severity and poor treatment control are inversely associated with QoL. In this study, 109 men (16-68 years) with 21OHD were included. The WHOQOL-BREF questionnaire was used to measure self-reported QoL domain scores on a 0-100 scale, where higher scores reflect better QoL. QoL domain scores were compared to published data on healthy and chronically ill reference populations from France, Germany, the Netherlands, and the United Kingdom. Differences in QoL scores among groups of disease severity and treatment control were tested within the study population. Overall, the men with CAH in this study appeared to rate their QoL as good. Median domain scores were 78.6 (IQR: 67.9-85.7) for physical health, 79.2 (IQR: 66.7-87.5) for psychological health, 75.0 (IQR: 58.3-83.3) for social relationships, and 81.3 (IQR: 71.9-90.6) for environment. In general, these scores were similar to WHOQOL-BREF domain scores in healthy references and higher compared to chronically ill reference populations. The domain scores did not differ among genotype groups, but patients with undertreatment or increased 17-hydroxyprogestrone concentrations scored higher on several QoL domains (p<0.05). Patients treated with dexamethasone or prednisone scored higher on the physical health, psychological health, and social relationships domains, but not on the environmental domain. In conclusion, QoL domain scores appeared to be comparable to healthy reference populations and higher compared to patients with a chronic illness. QoL was not influenced by genotype, but undertreatment and use of dexamethasone or prednisone were associated with higher QoL