Prevalence and incrimination of Anopheles fluviatilis species S (Diptera: Culicidae) in a malaria endemic forest area of Chhattisgarh state, central India

BACKGROUND: Chhattisgarh state in central India is highly endemic for malaria and contributes about 13% of annually reported malaria cases in the country with predominance of P. falciparum. Entomological investigations were carried out in a tribal forested area of district Bastar located in the southern part of Chhattisgarh state to record the prevalence of sibling species of Anopheles fluviatilis and An. culicifacies complexes. The vector species complexes were investigated at sibling species level for their biology in terms of resting and feeding behavior and malaria transmission potential. METHODS: Indoor resting vector mosquitoes collected during 2010–2011 were identified to sibling species by cytotaxonomy and polymerase chain reaction (PCR) assay. The blood meal source analysis and incrimination studies were done at sibling species level by counter current immunoelectrophoresis and enzyme linked immunosorbent assay (ELISA) respectively. RESULTS: Analysis of sibling species composition revealed predominance of An. fluviatilis species S in the study area, which was found to be highly anthropophagic and rested in human dwellings whereas the sympatric species T was primarily zoophagic. Incrimination studies showed high sporozoite rate in species S, thereby confirming its vectorial efficiency. An. culicifacies was encountered in low numbers and comprised species B and C in almost equal proportion. Both these species were found to be exclusively zoophagic. CONCLUSION: The observations made strongly suggest that species S of Fluviatilis Complex is the principal vector of malaria in certain forest areas of district Bastar, Chhattisgarh state and should be the target species for vector control operation. Vector control strategies based on biological characteristics of Fluviatilis S will lead to substantial decline in malaria incidence in such areas

Ab initio linear response and frozen phonons for the relaxor PMN (PbMg1/3Nb2/3O3)

We report first principles density functional studies using plane wave basis sets and pseudopotentials and all electron linear augmented plane wave (LAPW) of the relative stability of various ferroelectric and antiferroelectric supercells of PMN for 1:2 chemical ordering along [111] and [001]. We used linear response with density functional perturbation theory (DFPT) as implemented in the code ABINIT to compute the Born effective charges, electronic dielectric tensors, long wavelength phonon frequencies and LO-TO splittings. The polar response is different for supercells ordered along [111] and [001]. Several polar phonon modes show significant coupling with the macroscopic electric field giving giant LO-TO splittings. For [111] ordering, a polar transverse optic (TO) mode with E symmetry is found to be unstable in the ferroelectric P3m1 structure and the ground state is found to be triclinic. Multiple phonon instabilities of polar modes and their mode couplings provide the pathway for polarization rotation. The Born effective charges in PMN are highly anisotropic and this anisotropy contributes to the observed huge electromechanical coupling in PMN solid solutions.Comment: 34 pages, 6 figures. to appear in PR

Studies on developmental variation of isoperoxidase and protein profile of Zea mays L.

The present study was aimed to produce the isoperoxidase and protein marker for the maize cultivars from India and to find the isoperoxidase and protein expression on the various developmental stages. The leaf samples of Zea mays L. were harvested on 3rd, 7th, 11th, 15th, 19th, 23rd and 27th d for electrophoretic analysis of isoperoxidase and protein. The total protein and isoperoxidase were isolated using the standard procedure described by Sadasivam and Manickam. Isozyme and protein separation was carried out using 10% non-denaturing polyacrylamide gel electrophoresis. The banding pattern suggested several similarities as well as presence of unique isoperoxidase and protein in each stage. These studies will be useful for the identification of the major proteins present at different developmental stages of Z. mays

Predicting the potrntial geographical distribution of the sugarcane wooly aphid using GARP and DIVA-GIS

This article does not have an abstract

Assessing biodiversity from space: an example from the Western Ghats, India

We demonstrate for the first time the potential use of satellite imagery to characterize areas of high and low species richness of trees in tropical forests. Our studies, conducted in the Biligiri Rangaswamy hills in the Western Ghats, India, show a high positive correlation between species richness and the Normalized Difference Vegetation Index (NDVI), which is an index of green biomass. We discuss the application of NDVI values for biodiversity assessment and monitoring, as well as for conservation planning. The NDVI is a rough measure of green biomass

Rattan species richness and population genetic structure of Calamus flagellum in North-Eastern Himalaya, India

Rattans, the climbing palms, are one of the most important non-wood forest produce supporting the livelihood of many forest dwelling communities in India. However, extensive harvest, loss of habitat and poor regeneration has resulted in dwindling of rattan populations necessitating an urgent need to conserve the existing rattan genetic resources. In this study, using GIS tools, an attempt has been made to develop species richness maps of rattans in the North-Eastern Himalaya, a mega-diversity region in India. At least four sites of extremely high species richness were identified that could be prioritized for in situ conservation. Further, using molecular tools, genetic variability was assessed in six populations of an economically important rattan, Calamus flagellum. The population that was least disturbed or harvested maintained comparatively higher levels of genetic diversity than those that were disturbed. The study, perhaps the first in the region, emphasizes the need for developing strategies for the long-term conservation of rattans in the North-Eastern Himalaya