The occurrence and significance of Pseudomonas aeruginosa isolated from some meat products in Sohag city.

Contamination of meat and meat products with pathogenic and spoilage microorganisms is one of the most important challenges facing the meat industry that results in a range of human health problems and economic losses. This work aimed to identify the occurrence of Pseudomonas spp. especially Pseudomonas aeruginosa (P. aeruginosa) in some processed and ready-to-eat meat products in Sohag governorate. A total of 200 random meat product samples; minced beef meat, luncheon, burger, and sausage (50 of each) were purchased from different markets in Sohag governorate, Egypt over a period of 12 months from November 2020 to October 2021. Pseudomonas spp. was suspected in 32 (15%) of the meat products examined samples using the colony morphology on Cetrimide agar, represented as follows; 30%, 18%, 6%, and 10% in minced beef meat, luncheon burger, and sausage, respectively. Using the morphological and biochemical methods, P. aeruginosa was suspected in 12 isolates (37.5%) with an incidence of 12/200 (6%) of the total examined samples. The PCR results revealed that only 8/12 (66.7%) of the suspected isolates encoded the 16S rDNA gene of P. aeruginosa with an incidence of 4% of the total examined samples, 4 (50%) of which were detected in the minced beef meat samples, 2 (25%) in the sausage samples while in the luncheon and burger P. aeruginosa was identified in only 1 sample (12.5%) for each

INFLUENCE OF NUTRITIONAL AND CLIMATIC CONDITIONS ON MYCELIAL GROWTH OF THREE OYSTER MUSHROOM STRAINS

The mycelial growth rate of three oyster mushroom strains namely Pleurotus erengii, P. ostreatus and P. florida was examined on six different agar media (malt extract, Potato dextrose, rose bengal, corn meal, czapek’s dox and waksman’s glucose agar media) to select the most suitable one. Effect of pH was also examined at different values (5.0, 5.5, 6.0, 6.5 and 7.0) on the selected medium. Results indicated that Malt extract agar medium was the most suitable one for mycelium growth of all the tested mushroom strains, being the highest (9 cm ) for P. erengii and P. ostreatus at pH 7 and 9 cm at pH 6 -6.5 for P. florida after 6 days of incubation. The mycelia growth of the tested oyster mushroom strains was also examined at different temperatures (15, 20, 25, 30 and 35ºC) and different levels of relative humidity (50, 65, 75, 85, 95 and 100 %) on malt extract agar medium. The highest mycelial growth rate was obtained at 25°C and relative humidity of 65% after 6 days of incubation for the three tested oyster mushrooms

Effect of some essential oils against Aeromonas hydrophila artificially inoculated into raw Nile Tilapia fish fillets during refrigeration storage.

Aeromonas spp. is one of the emerging foodborne pathogens that gained importance during the last decades because of its zoonotic potential and as one of the specific spoilage organisms in seafood products. Agar well-diffusion assay revealed that cinnamon EO-Trans Cinnamaldehyde- (TC) and garlic Eos (GEO) showed the highest zone of inhibition against Aeromonas strains 18 mm for each at 0.5 % followed by thyme EO (TEO) (12 mm). While Clove EO (CEO) and onion EO (OEO) didn’t inhibit the growth of Aeromonas spp. Resazurin microtiter plate assay indicated that the MIC values were 3.125 mg/ml for GEO, 6.25 mg/ml for TC, 12.5 mg/ml for CEO, 50 mg/ml for TEO, and 75 mg/ml for OEO. The application of cinnamon, clove, garlic, thyme, and onion Eos against Aeromonas spp. on tilapia fish fillets stored at 4oC showed that among the low concentration of Eos (25 mg/ml), cinnamon and clove Eos showed a significant reduction in Aeromonas counts. Also, the higher concentration of CEO (25 mg/ml) caused a significant reduction rate. Counts of A. hydrophila in tilapia fish fillets of Eos treated samples were significantly different compared to the initial counts. EOS showed a significant reduction in the PH value of fish fillets except for the lower concentrations of TEO & OEO (alpha =0.052, P>0.05). A shelf-life extension of 2-3 days was achieved with essential oils treatment. They could be recommended as natural antimicrobial control of A. hydrophila in fish fillets

Detection of Salmonella species in chicken carcasses using genus specific primer belong to invA gene in Sohag city, Egypt

Aim: This study aimed to detect Salmonella species found as contaminants in chicken carcass (thigh, breast, wings, liver, and gizzard). Materials and Methods: A total of 75 chicken samples including thigh, breast, wings, liver, and gizzard (15 of each) were collected from different markets in Sohag city for detection of Salmonella species by culture methods, biochemical tests, serology, and polymerase chain reaction. Results: The overall incidence of Salmonella contamination of 75 examined samples was found to be 6.6% with the higher percentage of Salmonella being isolated from liver samples (13.3%) followed by thigh, wings, gizzard (6.6%) while breast show negative result. Conclusion: Results in this study indicate that contamination of chicken carcass with Salmonella needs strict hygienic measures to prevent their transmission to human

Draft genome sequence of plant growth-promoting bacillus altitudinis strain pae4

We report here the draft genome of Bacillus altitudinis strain PAE4, a thermophilic plant growth-promoting rhizobacterium isolated from the coastal ridge of the Mediterranean Sea in Egypt. Besides heat shock protein genes, several genes encoding phytobeneficial properties were identified

Phenolics from Nigella sativa L. straw: Characterization and insecticidal activity against Agrotis ipsilon (Hüfnagel)

The excessive production of food and agro-waste has become a significant problem for society, the economy, and the environment. To meet the growing demand for food free from harmful synthetic insecticides, a recent study has investigated the potential use of an ethanolic extract obtained from the straw of Nigella sativa L., a byproduct of seed collection, as a bioinsecticide. The study also evaluated its in-vitro and in-silico acetylcholinesterase (AChE) inhibitory potential against the Agrotis ipsilon (Hufnagel) moth species, which is known to cause damage to various crops and ornamental plants. The high-performance liquid chromatography examination revealed that the ethanolic N. sativa straw extract contained 18 phenolics, including 3 simple phenols, 8 phenolic acids, and 7 flavonoids. Catechol (330.14 μg/ml), chlorogenic (169.23 μg/ml), and gallic (110.93 μg/ml) acids were the predominant phenolics. On the other hand, catechin (94.07 μg/ml), naringenin (91.99 μg/ml), and rutin (78.16 μg/ml) were the major flavonoids identified in the extract. The insecticidal activity of the extract against the 4th larval instar of A. ipsilon was evaluated using four concentrations (1.25–10 %). The study found that higher extract concentrations led to increased mortality in the larvae. Specifically, the concentration of 10 % resulted in the highest mortality rate of 96.67 %. Lower concentrations of 5 %, 2.5 %, and 1.25 % resulted in mortality rates of 51.11 %, 18.89 %, and 9.17 %, respectively. The extract also showed higher activity against AChE in larval tissue, with an inhibition percentage of 65.2 % after 24 h of treatment. Docking experiments confirmed that ellagic acid and apigenin had higher binding affinity than the control (lanate). These results demonstrate the potential of utilizing agricultural waste like N. sativa straw to create innovative and sustainable bioinsecticides

Effect of Foot Reflexology on Clinical Outcomes of Neonates with Hyperbilirubinemia

Background and objectives: Neonatal hyperbilirubinemia is the most common reason for admission to Neonatal Intensive Care Unit (NICU) that can progress to critical levels. So, foot reflexology is one of the most widely used treatments and it is effective not only in the treatment of illnesses but also in maintaining health. Objectives: This study aimed to determine the effect of foot reflexology on the clinical outcomes of neonates with hyperbilirubinemia.Methods: A quasi-experimental research design was utilized in this study. The study subjects were 60 neonates with hyperbilirubinemia, recruited from NICU in Assiut University Children's Hospital. They were selected randomly and divided into two groups: the study group received foot reflexology interventions and the control group received routine care. Two tools were used to collect the necessary data, which included a personal structure questionnaire and neonataloutcomes assessment sheet.Results: On the fifth day, the mean of total serum bilirubin over the study group was 7.89±1.12 mg/dl, and over the control group was 10.55±0.55 mg/dl. According to the mean of the duration of phototherapy and hospital stay, the study group was 3.86±0.86- 6.80±0.88 respectively, but over the control group was 5.33±0.75- 10.40±1.22 respectively.Conclusions: A statistically significant positive correlation between applying foot reflexology interventions and improving clinical outcomes of neonates with hyperbilirubinemia. We recommended that in-service training and education programs for neonatal nurses should be conducted on a regular basis to teach them non-pharmacological strategies such as foot reflexology techniques

Effect of bio-stimulants on growth and chemical composition of mahogany seedlings grown under treated wastewater (TWW)

A pot experiment was carried out to study the effect of treated waste water (TWW) on mahogany seedlings and the role of bio-stimulants in alleviating the harmful impacts, using –6- months' old seedlings.  The TWW was used in 3 types coded as 75%WW/25%PW, 50%WW/50%PW and 25%WW/75%PW, according the ratio between treated wastewater (WW) and potable water (PW).  All seedlings grown under the different types of TWW treatments were sprayed with some bio stimulants. The results revealed that irrigating mahogany seedlings with 25WW/ 75PW, significantly increased plant height, improved the formation of leaves, fresh weight leaves, 25WW/ 75PW increased total indoles whereas using 75WW/25PW or 50WW/50PW, for irrigation produced thicker and heavier FW of stems. FW of leaves with 50WW/50PW gave the heaviest fresh and dry weights of leave and roots diameter, total phenols, free proline and total soluble sugars. The treatment of 75WW/25PW formed significantly longer roots, both 75WW/25PW 0r 50WW/50PW, insignificantly increased fresh weight of roots. All bio-stimulants significantly increased plant height and seaweed extract, salicylic acid and Pot. silicate were the most effective. PS treatment exhibited the thickest stems.&nbsp