Study of a model for the folding of a small protein

We describe the results obtained from an improved model for protein folding. We find that a good agreement with the native structure of a 46 residue long, five-letter protein segment is obtained by carefully tuning the parameters of the self-avoiding energy. In particular we find an improved free-energy profile. We also compare the efficiency of the multidimensional replica exchange method with the widely used parallel tempering.Comment: typos corrected, one figure adde

Seismic Vulnerability Assessment of a Historical Church: Limit Analysis and Nonlinear Finite Element Analysis

The seismic vulnerability of a historical Basilica church located in Italy is studied by means of limit analysis and nonlinear finite element (FE) analysis. Attention is posed to the failure mechanisms involving the façade of the church and its interaction with the lateral walls. In particular, the limit analysis and the nonlinear FE analysis provide an estimate of the load collapse multiplier of the failure mechanisms. Results obtained from both approaches are in agreement and can support the selection of possible retrofitting measures to decrease the vulnerability of the church under seismic loads

Single-channel analysis of a ClC-2-like chloride conductance in cultured rat cortical astrocytes

AbstractThe single-channel behavior of the hyperpolarization-activated, ClC-2-like inwardly rectifying Cl− current (IClh), induced by long-term dibutyryl-cyclic-AMP-treated cultured cortical rat astrocytes, was analyzed with the patch-clamp technique. In outside-out patches in symmetrical 144 mM Cl− solutions, openings of hyperpolarization-activated small-conductance Cl− channels revealed burst activity of two equidistant conductance levels of 3 and 6 pS. The unitary openings displayed slow activation kinetics. The probabilities of the closed and conducting states were consistent with a double-barrelled structure of the channel protein. These results suggest that the astrocytic ClC-2-like Cl− current IClh is mediated by a small-conductance Cl− channel, which has the same structural motif as the Cl− channel prototype ClC-0

Regulating Smart Robots and Artificial Intelligence in the European Union

Objective: In recent years, the need for regulation of robots and Artificial Intelligence has become apparent in Europe. European Union needs a standardized regulation that will ensure a high level of security in robotics systems to prevent potential breaches. Therefore a new regulation should make clear that it is the responsibility of producers to identify the blind spots in these systems, exposing their flaws, or, when a vulnerability is discovered in a later stage, to update the system even if that model is not on the market anymore. This article aims at suggesting some possible revisions of the existing legal provisions in the EU.Methods: The author employed the Kestemont legal methodology, analyzing legal text, comparing them, and connecting them with technical elements regarding smart robots, resulting in the highlighting of the critical provisions to be updated.Results: This article suggests some revisions to the existing regulatory proposals: according to the author, although the AI Act and the Cyberresilience Act represent a first step towards this direction, their general principles are not sufficiently detailed to guide programmers on how to implement them in practice, and policymakers should carefully assess in what cases lifelong learning models should be allowed to the market. The author suggests that the current proposal regarding mandatory updates should be expanded, as five years are a short time frame that would not cover the risks associated with long-lasting products, such as vehicles.Scientific novelty: The author has examined the existing regulatory framework regarding AI systems and devices with digital elements, highlighted the risks of the current legal framework, and suggested possible amendments to the existing regulatory proposals.Practical significance: The article can be employed to update the existing proposals for the AI Act and the Cyber-resilience Act

Legal Aspects of the Use Artificial Intelligence in Telemedicine

Objective: the rapid expansion of the use of telemedicine in clinical practice and the increasing use of Artificial Intelligence has raised many privacy issues and concerns among legal scholars. Due to the sensitive nature of the data involved particular attention should be paid to the legal aspects of those systems. This article aimed to explore the legal implication of the use of Artificial Intelligence in the field of telemedicine, especially when continuous learning and automated decision-making systems are involved; in fact, providing personalized medicine through continuous learning systems may represent an additional risk. Particular attention is paid to vulnerable groups, such as children, the elderly, and severely ill patients, due to both the digital divide and the difficulty of expressing free consent.Methods: comparative and formal legal methods allowed to analyze current regulation of the Artificial Intelligence and set up its correlations with the regulation on telemedicine, GDPR and others.Results: legal implications of the use of Artificial Intelligence in telemedicine, especially when continuous learning and automated decision-making systems are involved were explored; author concluded that providing personalized medicine through continuous learning systems may represent an additional risk and offered the ways to minimize it. Author also focused on the issues of informed consent of vulnerable groups (children, elderly, severely ill patients).Scientific novelty: existing risks and issues that are arising from the use of Artificial Intelligence in telemedicine with particular attention to continuous learning systems are explored.Practical significance: results achieved in this paper can be used for lawmaking process in the sphere of use of Artificial Intelligence in telemedicine and as base for future research in this area as well as contribute to limited literature on the topic

Acute pulmonary hypertension caused by tumor embolism: a report of two cases.

Acute pulmonary hypertension leading to right ventricular failure and circulatory collapse is usually caused by thromboembolic obstruction of the pulmonary circulation. However, in rare instances, other causes can be associated with a similar clinical presentation. We present and discuss the clinical histories of two patients with acute right ventricular failure due to an atypical cause of pulmonary hypertension, disseminated pulmonary tumor embolism