11 research outputs found
Site-specific incorporation of phosphotyrosine using an expanded genetic code.
Get PDFAccess to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination
Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response
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Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions
No full textCell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
No full textSpin-label scanning reveals conformational sensitivity of the bound helical interfaces of IA<sub>3</sub>
No full textPptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis
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