Light Plays an Essential Role in Intracellular Distribution of Auxin Efflux Carrier PIN2 in Arabidopsis thaliana

BACKGROUND: Light plays a key role in multiple plant developmental processes. It has been shown that root development is modulated by shoot-localized light signaling and requires shoot-derived transport of the plant hormone, auxin. However, the mechanism by which light regulates root development is not largely understood. In plants, the endogenous auxin, indole-3-acetic acid, is directionally transported by plasma-membrane (PM)-localized auxin influx and efflux carriers in transporting cells. Remarkably, the auxin efflux carrier PIN proteins exhibit asymmetric PM localization, determining the polarity of auxin transport. Similar to PM-resident receptors and transporters in animal and yeast cells, PIN proteins undergo constitutive cycling between the PM and endosomal compartments. Auxin plays multiple roles in PIN protein intracellular trafficking, inhibiting PIN2 endocytosis at some concentrations and promoting PIN2 degradation at others. However, how PIN proteins are turned over in plant cells is yet to be addressed. METHODOLOGY AND PRINCIPLE FINDINGS: Using laser confocal scanning microscopy, and physiological and molecular genetic approaches, here, we show that in dark-grown seedlings, the PM localization of auxin efflux carrier PIN2 was largely reduced, and, in addition, PIN2 signal was detected in vacuolar compartments. This is in contrast to light-grown seedlings where PIN2 was predominantly PM-localized. In light-grown plants after shift to dark or to continuous red or far-red light, PIN2 also accumulated in vacuolar compartments. We show that PIN2 vacuolar targeting was derived from the PM via endocytic trafficking and inhibited by HY5-dependent light signaling. In addition, the ubiquitin 26S proteasome is involved in the process, since its inhibition by mutations in COP9 and a proteasome inhibitor MG132 impaired the process. CONCLUSIONS AND SIGNIFICANCE: Collectively, our data indicate that light plays an essential role in PIN2 intracellular trafficking, promoting PM-localization in the presence of light and, on the other hand, vacuolar targeting for protein degradation in the absence of light. Based on these results, we postulate that light regulation of root development is mediated at least in part by changes in the intracellular distribution of auxin efflux carriers, PIN proteins, in response to the light environment

Search for anomalous production of di-lepton events with missing transverse momentum in e(+)e(-) collisions at root s = 161 and 172 GeV

Events containing a pair of charged leptons and significant missing transverse momentum are selected from a data sample corresponding to a total integrated luminosity of 20.6 pb^-1 at centre-of-mass energies of 161 GeV and 172 GeV. The observed number of events, four at 161 GeV and nine at 172 GeV, is consistent with the number expected from Standard Model processes, predominantly arising from W+W- production with each W decaying leptonically. This topology is also an experimental signature for the pair production of new particles that decay to a charged lepton accompanied by one or more invisible particles. Further event selection criteria are described that optimise the sensitivity to particular new physics channels. No evidence for new phenomena is observed and limits on the production of scalar charged lepton pairs and other new particles are presented

Tests of the standard model and constraints on new physics from measurements of fermion-pair production at 130-172GeV at LEP

Production of events with hadronic and leptonic final states has been measured in e(+)e(-) collisions at centre-of-mass energies of 130-172 GeV, using the OPAL detector at LEP. Cross-sections and leptonic forward-backward asymmetries are presented, both including and excluding the dominant production of radiative Z gamma events, and compared to Standard Model expectations. The ratio R-b of the cross-section for production to the hadronic cross-section has been measured. In a model-independent fit to the Z lineshape, the data have been used to obtain an improved precision on the measurement of gamma-Z interference. The energy dependence of alpha(em) has been investigated. The measurements have also been used to obtain limits on extensions of the Standard Model described by effective four-fermion contact interactions, to search for t-channel contributions from new massive particles and to place limits on gaugino pair production with subsequent decay of the gaugino into a light gluino and a quark pair

New insights into the regulation of greening and carbon-nitrogen balance by sugar metabolism through a plastidic invertase

Since the photosynthetic apparatus of plants contains a massive amount of nitrogen, the regulation of its development by sugar signals is important to the maintenance of the carbon-nitrogen balance. Recently, we isolated a new Arabidopsis mutant, sicy (sugar-inducible cotyledon yellow)-192, whose cotyledons were prevented from greening by treatment with sucrose. On treatment with sucrose, the expression of photosynthesis- and nitrogen assimilation-related genes was respectively weaker and stronger in the mutant seedlings than the wild-type seedlings. In the mutants, the gene encoding plastidic alkaline/neutral (A/N) invertase (INV-E) was point-mutated at codon 294, with Tyr substituted for Cys (C294Y). These findings provide new insights into the regulation of greening and carbon-nitrogen balance by sugar metabolism through INV-E in plastids. In this addendum, we describe the phenotypes of sicy-192 on treatment with sucrose in more detail, and propose a possible relationship among sugar metabolism through INV-E, plastid-to-nucleus retrograde signaling, and ethylene, a plant hormone, in the regulation of plant development and metabolism