20 research outputs found
INFLUENCE OF INFRALOW-FREQUENCY MAGNETIC-FIELD ON THE NERVOUS CELLS RHYTHM AND THEIR TOLERANCE TO HYPOXIA
The direct effect of infralow-frequency (0,05, 0,1, 0,25 Hz, 100 nT) magnetic fields (MF) was demonstrated on the brain cellular-tissue model-surviving slices of mouse cerebellum. MF influence is a trigger for the nervous cells. MF-5 Hz revealed two-phases response: inhibition and excitation of the impulse activity of neurons. Besides that we recorded convulsive effect of MF. The experiments with simultaneous exposure of hypoxia and MF revealed a prohypoxia effect of MF, when the oxygen concentration was very low and also after reoxygenation. The surviving slices may be used as a model for studying the fine mechanisms of influence of different intensity MF on the nervous cells
Individual resistance of the organism and nerve cell to hypoxia
The correlation between the pattern of a neuron's reaction to acute hypoxia and individual resistance to oxygen deficit is studied on rats in vivo as well as on surviving slices of their cerebellum in vitro. According to the survival time in a pressure chamber simulating an altitude of 11 km all the rats were divided into groups of high resistance, medium resistance, and low resistance to hypoxia. Survival time was 4.2 times longer in the high resistance group than in the low resistance group. In the cerebellar slices of high resistance animals 61.5% high-resistance neurons and 38.5% low-resistance neurons were recorded. On the other hand, in the high resistance animals the percentage of high-resistance neurons and low-resistance neurons was 31.2 and 68.8, respectively. The period of hypoxia development was 4.32 times longer in the high-resistance neurons as compared to low-resistance neurons. It is speculated that individual differences in the resistance to O2 deficit are of a hereditary nature and manifest themselves not only on the level of the whole organism, but also in the individual nerve cell. © 1995 Plenum Publishing Corporation
INFLUENCE OF INFRALOW-FREQUENCY MAGNETIC-FIELD ON THE NERVOUS CELLS RHYTHM AND THEIR TOLERANCE TO HYPOXIA
The direct effect of infralow-frequency (0,05, 0,1, 0,25 Hz, 100 nT) magnetic fields (MF) was demonstrated on the brain cellular-tissue model-surviving slices of mouse cerebellum. MF influence is a trigger for the nervous cells. MF-5 Hz revealed two-phases response: inhibition and excitation of the impulse activity of neurons. Besides that we recorded convulsive effect of MF. The experiments with simultaneous exposure of hypoxia and MF revealed a prohypoxia effect of MF, when the oxygen concentration was very low and also after reoxygenation. The surviving slices may be used as a model for studying the fine mechanisms of influence of different intensity MF on the nervous cells
EFFECTS OF SOME PHARMACOLOGICAL AGENTS ON ELECTRICAL-ACTIVITY OF THE CEREBELLUM SLICES UNDER HYPOXIA
[No abstract available
Effects of some pharmacological agents on electrical activity of the cerebellum slices under hypoxia
[No abstract available
EFFECTS OF SOME PHARMACOLOGICAL AGENTS ON ELECTRICAL-ACTIVITY OF THE CEREBELLUM SLICES UNDER HYPOXIA
Features of disorders in the hemostasis system in acute peritonitis on the background of diabetes mellitus
Background. Until now, acute peritonitis remains in the focus of attention in
modern abdominal surgery, which is due to the preservation of a high level of morbidity
and mortality, as well as high treatment costs. Materials and methods. 46 patients with
acute appendicitis complicated by peritonitis were studied, divided into 2 groups: the first
(n=28) – without concomitant diabetes mellitus; and the second (n = 18) – with diabetes
mellitus (mainly of the first type). The patients were operated on: appendectomy, sanitation
and drainage of the abdominal cavity were performed. Methods: assessment of the state of
the hemostasis system, platelet aggregation activity, their lipid composition, the severity of
oxidative processes and phospholipase activity. Follow-up period: before surgery, 2 and 5
days after surgery. Results. It was found that in acute peritonitis, there was an increase in
the level of diene conjugates and malondialdehyde in the blood plasma at the observation
stages by 70.8, 54.2 and 98.6, 82.3% (Ñ€ < 0.05), a decrease in the activity of superoxide
dismutase by 37.5 and 33.1% (Ñ€ < 0.05), an increase in the activity of phospholipase A2 by
141.7 and 44.8% (Ñ€ < 0.05). During hospitalization of patients to the clinic, an increase in
the content of cholesterol esters, cholesterol, triglycerides in platelets was noted by 13.9,
13.6, 20.9% (Ñ€ < 0.05), respectively. An increase in the degree of platelet aggregation was
revealed by 18.3% (Ñ€ < 0.05). Coagulation time was reduced by 21.1% (Ñ€ < 0.05), clot formation
– by 22.4% (р < 0.05). The fibrinolysis time was prolonged by 21.2% (р < 0.05).
Comparative analysis has shown that in diabetes mellitus there are large violations in the
system of hemostasis and homeostasis in general. In patients of this group, the content of
diene conjugates and malondialdehyde in blood plasma exceeded those of the comparison
group at all stages of observation by 22.0-23.4 and 15.1-18.0% (Ñ€ < 0.05), the activity of
superoxide dismutase decreased by 17.1-18.3% (Ñ€ < 0.05), phospholipase A2 increased by
44.8-42.5% (Ñ€ < 0.05).<0.05). Changes in the lipid spectrum of platelets increased: cholesterol
levels were higher by 14.7% (р < 0.05), triglycerides – by 21.2% (р < 0.05), free fatty
acids – by 19.3% (р < 0.05). Comparative analysis of the hemostasis system showed that
the coagulation reaction time in this group on the 2nd and 5th days decreased by 14.2 and
26.3% (Ñ€ < 0.05), the time of clot formation - by 15.1 and 13.6% (Ñ€ < 0.05), the percentage
of lysis – by 19.4 and 12.5% (р < 0.05). Conclusions. One of the significant factors in the
modulation of the hemostasis system in comorbid pathology of patients with acute peritonitis
is a significant activation of trigger agents leading to oxidative stress, excessive phospholipase activity, changes in the composition of platelet lipids, hypercoagulemia, inhibition
of fibrinolytic activity, microthrombosis. This is especially evident in conditions burdened
with diabetes mellitus, in which the activity of hemocoagulation triggers and their effect
on the hemostasis system is detected to a greater extent