Efeito da reinoculação e da adubação nitrogenada no rendimento da soja em Mato Grosso.

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Global embedding of D-dimensional black holes with a cosmological constant in Minkowskian spacetimes: Matching between Hawking temperature and Unruh temperature

We study the matching between the Hawking temperature of a large class of static D-dimensional black holes and the Unruh temperature of the corresponding higher dimensional Rindler spacetime. In order to accomplish this task we find the global embedding of the D-dimensional black holes into a higher dimensional Minkowskian spacetime, called the global embedding Minkowskian spacetime procedure (GEMS procedure). These global embedding transformations are important on their own, since they provide a powerful tool that simplifies the study of black hole physics by working instead, but equivalently, in an accelerated Rindler frame in a flat background geometry. We discuss neutral and charged Tangherlini black holes with and without cosmological constant, and in the negative cosmological constant case, we consider the three allowed topologies for the horizons (spherical, cylindrical/toroidal and hyperbolic).Comment: 7 pages; ReVTeX

Global phylogeography and evolution of chelonid fibropapilloma-associated herpesvirus

A global phylogeny for chelonid fibropapilloma-associated herpesvirus (CFPHV), the most likely aetiological agent of fibropapillomatosis (FP) in sea turtles, was inferred, using dated sequences, through Bayesian Markov chain Monte Carlo analysis and used to estimate the virus evolutionary rate independent of the evolution of the host, and to resolve the phylogenetic positions of new haplotypes from Puerto Rico and the Gulf of Guinea. Four phylogeographical groups were identified: eastern Pacific, western Atlantic/eastern Caribbean, mid-west Pacific and Atlantic. The latter comprises the Gulf of Guinea and Puerto Rico, suggesting recent virus gene flow between these two regions. One virus haplotype from Florida remained elusive, representing either an independent lineage sharing a common ancestor with all other identified virus variants or an Atlantic representative of the lineage giving rise to the eastern Pacific group. The virus evolutionary rate ranged from 1.62x10(-4) to 2.22x10(-4) substitutions per site per year, which is much faster than what is expected for a herpesvirus. The mean time for the most recent common ancestor of the modern virus variants was estimated at 192.90-429.71 years ago, which, although more recent than previous estimates, still supports an interpretation that the global FP pandemic is not the result of a recent acquisition of a virulence mutation(s). The phylogeographical pattern obtained seems partially to reflect sea turtle movements, whereas altered environments appear to be implicated in current FP outbreaks and in the modern evolutionary history of CFPHV.DNER-PR; US NMFS (NMFS-NOAA) [NA08NMF4720436]; US-Fish and Wildlife Service (USFWS); Sociedad Chelonia; WIDECAST; US Environmental Protection Agency (US-EPA); Lisbon Oceanarium, Portugal; Interdisciplinary Research Center for Animal Health of the Faculty of Veterinary Medicine of the Technical University of Lisbon (FMV/TUL)info:eu-repo/semantics/publishedVersio

Adubação nitrogenada na cultura da soja.

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Contraction of fully expanded FMR1 alleles to the normal range: predisposing haplotype or rare events?

Fragile X syndrome (FXS), the most common cause of inherited intellectual disability, is due to the expansion over 200 CGGs and methylation of this polymorphic region, in the 5'-UTR (untranslated region) of FMR1 (Xq27.3). We have identified four FXS mosaic males: M1-(CGG)35/(CGG)>200; M2-(CGG)26/(CGG)>200; M3-(CGG)39/(CGG)>200; and M4-(CGG)18/(CGG)125/(CGG)>200. After genotyping their respective mothers, we suggested that normal alleles of these patients resulted from post-zygotic contractions of full expansions. The detection of these four rare independent cases led us to hypothesize the existence of a large-contraction predisposing haplotype in our population. Next, we questioned whether other normal pure CGGs would have arisen through similar contractions from fully expanded alleles. To address these questions, we identified stable single-nucleotide polymorphism (SNP) lineages and related short tandem repeat (STR) haplotypes (DXS998-DXS548-FRAXAC1-FRAXAC2) of the four mosaics, 123 unrelated FXS patients and 212 controls. An extended flanking haplotype (34-44-38-336) shared by mosaics from lineage A suggested a risk lineage-specific haplotype more prone to large contractions. Other normal pure FMR1 alleles from this SNP background also shared phylogenetically close STR haplotypes, although a single (CGG)exp>(CGG)24 contraction or the loss of AGG interruptions may explain their origin. In both scenarios, multistep FMR1 mutations involving the gain or loss of several CGGs seem to underlie the evolution of the repeat.We acknowledge all patients and their families for participating in this study, and medical geneticists Ana Maria Fortuna, Mariana Soeiro e Sa Oana Moldovan and Ana Berta Sousa for referring the patients. UMIB is funded by National Funds through FCT Foundation for Science and Technology, under the Pest-OE/SAU/UI0215/2014. SM is funded by the project IF/00930/2013/CP1184/CT0002 from FCT. IM received a research grant 2014 DEFI-CHP, E.P.E

Development of poly (lactic-co-glycolic acid)/bioglass fibers using an electrospinning technique.

This work establishes experimental conditions for the synthesis of polymeric fibers using the electrospinning technique, modifying some operational parameters such as drum collector rotation speed and applied voltage were modified. The samples were obtained from a copolymer of poly(lactic-co-glycolic acid) (PLGA), with a molecular weight of approximately 220,000 g/mol, dissolved in a dimethyl sulfoxide/dichloromethane (3:1 v/v) solution. With the aim of developing scaffolds for bone tissue engineering, we added a calcium phosphate glass based on 44.5Ca0-44.5P2O5-11Na2O to the fibrous PLGA structures. The preliminary characterization of these PLGA structures with and without the addition of biodegradable glass was performed using SEM analysis. We found the formation of aligned and homogeneous fibers with an average diameter of 5.2 ± 2.1 µm when using the 800 rpm - 8cm - 9 kV parameters. In addition, the incorporation of 1 wt% of previously silanized calcium phosphate particles substantially altered the fiber morphology and porosity. However, a substantial increase in the bioactivity of the composite was observed in contrast with the unmodified PLGA

Mercury accumulation from food decreases collembolans' growth

In the terrestrial environment, mercury (Hg) contamination can be originated from different inorganic and metal-organic sources, redistributed and transformed in soils. In the present study, the effects of contaminated food with environmentally relevant concentrations of Hg were evaluated in the soil-dwelling invertebrate Folsomia candida. Changes in growth rate and Hg bioaccumulation levels were observed at different concentrations of Hg in food, which can be complementary for data already available for reproduction and survival from standardized protocols. Collembolan growth was recorded every two days, and their growth rate along with a Von Bertalanffy's growth curve were derived showing that growth was dependent on Hg food concentration. Also, the final length of animals reflected the Hg concentration in food, with differences in all treatments comparing to non-exposed organisms. Toxicokinetic patterns from different Hg concentrations in food were not significantly different during the uptake phase, but differences were found in the depuration phase. Combining the two approaches, collembolans seem to invest their energy for depuration processes, neglecting other vital processes, such as growth. Also, contaminated food avoidance possibly occurred, thus decreasing their feeding and contaminant intake. Therefore, growth tests in collembolans can act as complementary tools to bioaccumulation and reproductive assays, towards a mechanistic understanding of how organisms use their energy upon contamination. Changes in growth rate, even at low and environmentally relevant concentrations, could be a warning signal when occurring in species with key roles in ecosystems. Also, this study highlights the importance of these complementary tests for a better and complete approach to risk assessment studies