HOW TO LIVE LIFE ALL THE WAY UP LEARNING LIFE SKILLS FROM LITERARY CHARACTERS

In this essay, using the theories of psychiatrist, Eric Berne and his script theory; psychoanalyst, Carl Jung and his archetypes and mandalas; as well as the Native American Medicine Wheel; and the Hindu notion of the kundalini, I analyze the psychological development of Adele Quested of E.M. Forster’s A Passage to India (1924) and Anna Wulf of Doris Lessing’s The Golden Notebook (1962). Adela Quested goes to India seeking the real India and while engaging the archetype of the Lover discovers her real Self. While in India she metaphorically walks the Medicine Wheel and discovers that to be whole she must balance her ability to think with her ability to feel. Anna Wulf’s psychological development requires her to walk the Medicine Wheel and discover that her idealistic thinking must be balanced by realistic thinking and her femininity with her masculinity. This is an arduous task for Anna and requires the help of the Destroyer archetype. By the end of the novels both women have rewritten their scripts and become individuated and whole

Parallel analysis of ribonucleotide-dependent deletions produced by yeast Top1 in vitro and in vivo

Ribonucleotides are the most abundant non-canonical component of yeast genomic DNA and their persistence is associated with a distinctive mutation signature characterized by deletion of a single repeat unit from a short tandem repeat. These deletion events are dependent on DNA topoisomerase I (Top1) and are initiated by Top1 incision at the relevant ribonucleotide 3′-phosphodiester. A requirement for the re-ligation activity of Top1 led us to propose a sequential cleavage model for Top1-dependent mutagenesis at ribonucleotides. Here, we test key features of this model via parallel in vitro and in vivo analyses. We find that the distance between two Top1 cleavage sites determines the deletion size and that this distance is inversely related to the deletion frequency. Following the creation of a gap by two Top1 cleavage events, the tandem repeat provides complementarity that promotes realignment to a nick and subsequent Top1-mediated ligation. Complementarity downstream of the gap promotes deletion formation more effectively than does complementarity upstream of the gap, consistent with constraints to realignment of the strand to which Top1 is covalently bound. Our data fortify sequential Top1 cleavage as the mechanism for ribonucleotide-dependent deletions and provide new insight into the component steps of this process

Made In America

This paper attempts to reveal how awareness of one\u27s affiliations and their group’s interactions with others could lead to equality for all co-culture groups in America. This is achieved through the analysis of an event at Pepperdine University via the Social Identity Theory

The 5'-3' exoribonuclease Pacman (Xrn1) regulates expression of the heat shock protein Hsp67Bc and the microRNA miR-277-3p in Drosophila wing imaginal discs

Pacman/Xrn1 is a highly conserved exoribonuclease known to play a critical role in gene regulatory events such as control of mRNA stability, RNA interference and regulation via miRNAs. Although Pacman has been well studied in Drosophila tissue culture cells, the biologically relevant cellular pathways controlled by Pacman in natural tissues are unknown. This study shows that a hypomorphic mutation in pacman (pcm5) results in smaller wing imaginal discs. These tissues, found in the larva, are known to grow and differentiate to form wing and thorax structures in the adult fly. Using microarray analysis, followed by quantitative RT-PCR, we show that eight mRNAs were increased in level by >2 fold in the pcm5 mutant wing discs compared to the control. The levels of pre mRNAs were tested for five of these mRNAs; four did not increase in the pcm5 mutant, showing that they are regulated at the post-transcriptional level and therefore could be directly affected by Pacman. These transcripts include one that encodes the heat-shock protein Hsp67Bc, which is upregulated 11.9-fold at the post-transcriptional level and 2.3-fold at the protein level. One miRNA, miR-277-3p, is 5.6-fold downregulated at the post-transcriptional level in mutant discs, suggesting that Pacman affects its processing in this tissue. Together, these data show that a relatively small number of mRNAs and miRNAs substantially change in abundance in pacman mutant wing imaginal discs. Since Hsp67Bc is known to regulate autophagy and protein synthesis, it is possible that Pacman may control the growth of wing imaginal discs by regulating these processes

KlNESlOLOGlCAL ANALYSIS OF OVERARM THROWING FOR ACCURACY WITH DOMINANT AND NON-DOMINANT ARMS

The main aim of this study was to examine differences kinematically between dominant and non-dominant overarm throws for accuracy. Fourty-nine right-handed primaly school students served as the subjects who were requested to make overarm throws with dominant and non-dominant arms. A three-dimensional Motion Analysis System was used to collect all kinematic data of overarm throwing while performance errors were recorded by a video camera. Performance errors and kinematic variables of right and lefl hands were compaired with Paired t-test and the relation among performance errors and kinematic variables was evaluated with canonical correlation. It was found that significant differences in the accuracy existed between dominant and non-dominant overarm throws. The dominant hand shows much better throwing performance in terms of accuracy. Kinematic analysis also indicated that there were significant differences in velocity and acceleration even though there was a great similarity in the timing of velocity and acceleration in overarm throws for accuracy