Spectroscopic and photometric analysis of the early-type spectroscopic binary HD 161853 in the centre of an H II region

We study the O-type star HD 161853, which has been noted as a probable double-lined spectroscopic binary system. We secured high-resolution spectra of HD 161853 during the past nine years. We separated the two components in the system and measured their respective radial velocities for the first time. We confirm that HD 161853 is an $\sim$1 Ma old binary system consisting of an O8 V star ($M_{\rm A,RV} \geq 22$ M$_\odot$) and a B1--3 V star ($M_{\rm B,RV} \geq 7.2$ M$_\odot$) at about 1.3 kpc. From the radial velocity curve, we measure an orbital period $P$ = 2.66765$\pm$0.00001 d and an eccentricity $e$ = 0.121$\pm$0.007. Its $V$-band light curve is constant within 0.014 mag and does not display eclipses, from which we impose a maximum orbital inclination $i=54$ deg. HD 161853 is probably associated with an H II region and a poorly investigated very young open cluster. In addition, we detect a compact emission region at 50 arcsec to HD 161853 in 22$\mu$m-WISE and 24$\mu$m-Spitzer images, which may be identified as a dust wave piled up by the radiation pressure of the massive binary system.Comment: 5 pages, 4 figures, to appear in A&

Novel flaviviruses from mosquitoes: Mosquito-specific evolutionary lineages within the phylogenetic group of mosquito-borne flaviviruses

Copyright © 2014 The Authors. Published by Elsevier Inc. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The attached file is the published version of the article

Spectroscopic and photometric analysis of the early-type spectroscopic binary HD 161853 in the centre of an H II region

Aims. We study the O-type star HD 161853, which has been noted as a probable double-lined spectroscopic binary system. Methods. We secured high-resolution spectra of HD 161853 during the past nine years. We separated the two components in the system and measured their respective radial velocities for the first time. Results. We confirm that HD 161853 is an ~1 Ma old binary system consisting of an O8 V star (MA,RV ≥ 22 M⊙) and a B1-3 V star (MB,RV ≥ 7.2 M⊙) at about 1.3 kpc. From the radial velocity curve, we measure an orbital period P = 2.66765 ± 0.00001 d and an eccentricity e = 0.121 ± 0.007. Its V-band light curve is constant within 0.014 mag and does not display eclipses, from which we impose a maximum orbital inclination i = 54 deg. HD 161853 is probably associated with an H ii region and a poorly investigated very young open cluster. In addition, we detect a compact emission region at 50 arcsec to HD 161853 in 22 μm-WISE and 24 μm-Spitzer images, which may be identified as a dust wave piled up by the radiation pressure of the massive binary system.Facultad de Ciencias Astronómicas y GeofísicasInstituto de Astrofísica de La Plat

Multiplex primer prediction software for divergent targets

We describe a Multiplex Primer Prediction (MPP) algorithm to build multiplex compatible primer sets to amplify all members of large, diverse and unalignable sets of target sequences. The MPP algorithm is scalable to larger target sets than other available software, and it does not require a multiple sequence alignment. We applied it to questions in viral detection, and demonstrated that there are no universally conserved priming sequences among viruses and that it could require an unfeasibly large number of primers (∼3700 18-mers or ∼2000 10-mers) to generate amplicons from all sequenced viruses. We then designed primer sets separately for each viral family, and for several diverse species such as foot-and-mouth disease virus (FMDV), hemagglutinin (HA) and neuraminidase (NA) segments of influenza A virus, Norwalk virus, and HIV-1. We empirically demonstrated the application of the software with a multiplex set of 16 short (10 nt) primers designed to amplify the Poxviridae family to produce a specific amplicon from vaccinia virus

Non-synchronous rotations in massive binary systems

Context. The OWN Survey has detected several O-type stars with composite spectra whose individual components show very different line broadening. Some of these stars have been revealed as binary systems whose components are asynchronous. This fact may be related to the processes acting in these systems (e.g., angular-momentum transfer, tidal forces, etc.) or to the origin of the binaries themselves. Aims. We aim to determine the orbital and physical parameters of the massive star HD 96264A in order to confirm its binary nature and to constrain the evolutionary status of its stellar components. Methods. We computed the spectroscopic orbit of the system based on the radial velocity analysis of 37 high-resolution, high-S/N, multi-epoch optical spectra. We disentangled the composite spectrum and determined the physical properties of the individual stellar components using FASTWIN

Novel insect-specific flavivirus isolated from northern Europe

Mosquitoes collected in Finland were screened for flaviviral RNA leading to the discovery and isolation of a novel flavivirus designated Hanko virus (HANKV). Virus characterization, including phylogenetic analysis of the complete coding sequence, confirmed HANKV as a member of the "insect-specific" flavivirus (ISF) group. HANKV is the first member of this group isolated from northern Europe, and therefore the first northern European ISF for which the complete coding sequence has been determined. HANKV was not transcribed as DNA in mosquito cell culture, which appears atypical for an ISF. HANKV shared highest sequence homology with the partial NS5 sequence available for the recently discovered Spanish Ochlerotatus flavivirus (SOcFV). Retrospective analysis of mitochondrial sequences from the virus-positive mosquito pool suggested an Ochlerotatus mosquito species as the most likely host for HANKV. HANKV and SOcFV may therefore represent a novel group of Ochlerotatus-hosted insect-specific flaviviruses in Europe and further afield