Cytokine and TIRC7 mRNA expression during acute rejection in cardiac allograft recipients

UNIFESP, Escola Paulista Med, Dept Pediat, São Paulo, SP, BrazilUNIFESP, Escola Paulista Med, Dept Med, São Paulo, SP, BrazilUNIFESP, Escola Paulista Med, Dept Pediat, São Paulo, SP, BrazilUNIFESP, Escola Paulista Med, Dept Med, São Paulo, SP, BrazilWeb of Scienc

T cell receptor excision circles (TRECs) in relation to acute cardiac allograft rejection

The purpose of this study was to quantify T cell receptor excision circles (TRECs) in blood mononuclear cells of cardiac transplant recipients and to investigate a possible relationship between TREC levels and rejection episodes. in addition, we investigated the correlation of TREC levels with age and also compared the levels between transplant recipients and healthy individuals. TREC levels were assessed by quantitative competitive PCR in 70 blood samples from 27 graft recipients and in 66 blood samples from 66 healthy individuals. the results showed: (1) higher TREC levels during rejection than during rejection-free periods (medians 9.0 vs. 0.3; p<0.001); (2) no suggestion of correlation with doses of prednisone or time after transplantation; (3) a negative correlation between TREC levels and age; and (4) lower TREC levels in cardiac recipients than in age-matched healthy controls. the value of blood TREC level measurements as an approach to rejection monitoring warrants future investigation.UNIFESP, Dept Pediat, Div Immunogenet, BR-04024003 São Paulo, BrazilUniversidade Federal de São Paulo, EPM, Dept Med, São Paulo, BrazilUNIFESP, Dept Pediat, Div Immunogenet, BR-04024003 São Paulo, BrazilUniversidade Federal de São Paulo, EPM, Dept Med, São Paulo, BrazilWeb of Scienc

CD27 but not CD70 and 4-1BB intragraft gene expression is a risk factor for acute cardiac allograft rejection in humans

UNIFESP, Paulista Sch Med, Dept Pediat, São Paulo, BrazilEscola Paulista Med, Dept Med, São Paulo, BrazilUNIFESP, Paulista Sch Med, Dept Pediat, São Paulo, BrazilEscola Paulista Med, Dept Med, São Paulo, BrazilWeb of Scienc