22 research outputs found

    Le choix matrimonial en Tunisie est-il transmissible?

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    La fréquence élevée des mariages endogames et consanguins en Tunisie se justifie par des traditions devenues règles de conduite dans la société pour des intérêts divers. Ces règles contribuent fortement à la détermination de la circulation des flux génétiques dans la population. Dans une enquête menée auprès de familles échantillonnées, nous avons comparé le type de choix matrimonial du père et de la mère avec celui de leurs enfants pour voir s¿il y a transmission de ce comportement d¿une génération à la suivante et analyser les causes. Un échantillon de 370 femmes âgées de 18 à 63 ans a été soumis à un questionnaire et a permis d¿établir un inventaire des unions consanguines chez les couples étudiés ainsi que chez leurs parents respectifs. Les unions consanguines des couples, tous degrés confondus représentent 32,71%. Elles atteignent 33,25% et 34,04% chez les parents. Ce sont les unions entre cousins germains qui sont les plus représentées, avec 16,21% pour le couple, 18,64% et 15,4% pour les parents. Une place particulière est réservée à l¿étude des unions avec la fille du frère du père. Des tableaux de contingence parents/enfants ont mis en évidence une ressemblance du choix matrimonial des enfants avec celui de leurs parents, appuyés par le de Pearson. Ce choix apparaît pour le mari comme pour la femme, significativement " transmissible " du comportement de leurs parents. La discussion de ces résultats aborde la transmission de ce phénomène au niveau de la population et ses causes environnementales. Elle souligne l¿importance de la place qu¿occupe l¿environnement social, économique et culturel dans la détermination de la circulation des flux génétiques dans les populations humaines et le maintien de ce modèle de famille dans la société

    Polycyclic aromatic hydrocarbon degradation and biosurfactant production by a newly isolated Pseudomonas sp. strain from used motor oil-contaminated soil

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    The aim of this study was to isolate and characterize a newly isolated bacterium, designated strain W10, a polycyclic aromatic hydrocarbon (PAHs) degrader and biosurfactant producer, belonged to Pseudomonas genus and closely related to Pseudomonas aeruginosa, with the 16 rRNA gene sequence similarity of 99.1%. Based on GC-MS analyses, it degraded around 80% of phenanthrene, used as the sole carbon and energy source, at an initial concentration of 200 mg l−1, after 30 days of incubation at 37 °C and 180 rpm, reducing the surface tension (ST) from 56.1 to 42 mN m−1 after 4 days of incubation. Furthermore, strain W10 utilized about 10%, 20%, 90%, and 99% of hexadecane (C16), pyrene, fluoranthene, and crude oil, respectively, after 30 days of incubation at 37 °C and 180 rpm. During the growth of strain W10 on phenanthrene and fluoranthene, some metabolites were identified, supporting the biodegradation pathways of the two PAHs. Interestingly, strain W10 showed also a significant potential to produce surface-active agents reducing the surface tension to 32 mN m−1 and reaching a production around 2 g l−1 after 48 h of incubation, in the presence of olive oil (1%,v/v) as substrate, at 37 °C and 180 rpm. Its biosurfactant, namely BSW10, showed an interesting emulsification activity and a high stability over a wide range of salinity (0–150 g l−1), temperature (0–100 °C), pH (2–12) and thus a promising abilities in used motor oil and crude oil removal from contaminated soils. Overall, these results provide evidence that strain W10 and its biosurfactant (BSW10) could be potential candidates for further bioremediation applications

    Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization

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    International audienceLittle is known about the molecular mechanisms that induce gametefusion during mammalian fertilization. After initial contact, adhesionbetween gametes only leads to fusion in the presence of threemembrane proteins that are necessary, but insufficient, for fusion:Izumo1 on sperm, its receptor Juno on egg and Cd9 on egg. Whathappens during this adhesion phase is a crucial issue. Here, wedemonstrate that the intercellular adhesion that Izumo1 creates with Juno is conserved in mouse and human eggs. We show that, along with Izumo1, egg Cd9 concomitantly accumulates in the adhesion area. Without egg Cd9, the recruitment kinetics of Izumo1 are accelerated. Our results suggest that this process is conservedacross species, as the adhesion partners, Izumo1 and its receptor,are interchangeable between mouse and human. Our findingssuggest that Cd9 is a partner of Juno, and these discoveries allowus to propose a new model of the molecular mechanisms leading togamete fusion, in which the adhesion-induced membraneorganization assembles all key players of the fusion machinery
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