A review of bioplastics and their adoption in the circular economy

The European Union is working towards the 2050 net-zero emissions goal and tackling the ever-growing environmental and sustainability crisis by implementing the European Green Deal. The shift towards a more sustainable society is intertwined with the production, use, and disposal of plastic in the European economy. Emissions generated by plastic production, plastic waste, littering and leakage in nature, insufficient recycling, are some of the issues addressed by the European Commission. Adoption of bioplastics–plastics that are biodegradable, bio-based, or both–is under assessment as one way to decouple society from the use of fossil resources, and to mitigate specific environmental risks related to plastic waste. In this work, we aim at reviewing the field of bioplastics, including standards and life cycle assessment studies, and discuss some of the challenges that can be currently identified with the adoption of these materials

Prevalence of hepatitis E virus in Italian pig herds. Preliminary results

Hepatitis E virus (HEV) is the causative agent of hepatitis E, and is an unenveloped positive sense single-stranded RNA wus. Swine HEV strains are genetically closely related to human strains from the same area, suggesting the occurrence of zoonotic transmission. Recently, human cases of hepatitis E have been linked to the consumption of raw or undercooked meat or organs from deer, wild boars or p1gs. The disease is now considered an emerging food-borne transmitted zoonosis. During 2006, a pilot investigation was performed to determine the prevalence of HEV in pig farms located in Northern Italy. 274 faecal samples were collected from healthy fattening animals (3-4 and 8-9 months of age) and from healthy breeding animals (gilts and sows) from 6 different farms, and analyzed using a Nested-RT-PCR targeting the open reading frame 2 (ORF2) region. Stool samples were suspended in water, and viral RNA extraction was performed using a commercial kit. Extracted viral RNA was subjected to RT-PCR amplification using degenerate primers conA 1-conS1 for the first amplification, and degenerate primers conA2-conS2 for the nested PCR, yielding a final fragment of 145 bp. HEV RNA was detected in sixty-nine of the 274 (25.2%) examined samples. None of the six farms resulted negative and the prevalence within the farms ranged between 2% and 60.5% For the characterization of the strains, randomly selected positive samples were subjected to nucleotide sequencing, and aligned with those present in the NCBI Data Bank Sequence analysis showed that all stra1ns were Swine Hepatitis E belonging to Genotype 3. These preliminary results confirm that swine HEV is widespread in Italian swine farms

The large cosmological constant approximation to classical and quantum gravity: model examples

We have recently introduced an approach for studying perturbatively classical and quantum canonical general relativity. The perturbative technique appears to preserve many of the attractive features of the non-perturbative quantization approach based on Ashtekar's new variables and spin networks. With this approach one can find perturbatively classical observables (quantities that have vanishing Poisson brackets with the constraints) and quantum states (states that are annihilated by the quantum constraints). The relative ease with which the technique appears to deal with these traditionally hard problems opens several questions about how relevant the results produced can possibly be. Among the questions is the issue of how useful are results for large values of the cosmological constant and how the approach can deal with several pathologies that are expected to be present in the canonical approach to quantum gravity. With the aim of clarifying these points, and to make our construction as explicit as possible, we study its application in several simple models. We consider Bianchi cosmologies, the asymmetric top, the coupled harmonic oscillators with constant energy density and a simple quantum mechanical system with two Hamiltonian constraints. We find that the technique satisfactorily deals with the pathologies of these models and offers promise for finding (at least some) results even for small values of the cosmological constant. Finally, we briefly sketch how the method would operate in the full four dimensional quantum general relativity case.Comment: 21 pages, RevTex, 2 figures with epsfi

A Discrete Time Presentation of Quantum Dynamics

Inspired by the discrete evolution implied by the recent work on loop quantum cosmology, we obtain a discrete time description of usual quantum mechanics viewing it as a constrained system. This description, obtained without any approximation or explicit discretization, mimics features of the discrete time evolution of loop quantum cosmology. We discuss the continuum limit, physical inner product and matrix elements of physical observables to bring out various issues regarding viability of a discrete evolution. We also point out how a continuous time could emerge without appealing to any continuum limit.Comment: 20 pages, RevTex, no figures. Additional Clarifications added. Version accepted for publication in Class. Quant. Gra

Nonminimal Couplings in the Early Universe: Multifield Models of Inflation and the Latest Observations

Models of cosmic inflation suggest that our universe underwent an early phase of accelerated expansion, driven by the dynamics of one or more scalar fields. Inflationary models make specific, quantitative predictions for several observable quantities, including particular patterns of temperature anistropies in the cosmic microwave background radiation. Realistic models of high-energy physics include many scalar fields at high energies. Moreover, we may expect these fields to have nonminimal couplings to the spacetime curvature. Such couplings are quite generic, arising as renormalization counterterms when quantizing scalar fields in curved spacetime. In this chapter I review recent research on a general class of multifield inflationary models with nonminimal couplings. Models in this class exhibit a strong attractor behavior: across a wide range of couplings and initial conditions, the fields evolve along a single-field trajectory for most of inflation. Across large regions of phase space and parameter space, therefore, models in this general class yield robust predictions for observable quantities that fall squarely within the "sweet spot" of recent observations.Comment: 17pp, 2 figs. References added to match the published version. Published in {\it At the Frontier of Spacetime: Scalar-Tensor Theory, Bell's Inequality, Mach's Principle, Exotic Smoothness}, ed. T. Asselmeyer-Maluga (Springer, 2016), pp. 41-57, in honor of Carl Brans's 80th birthda

Tumor Necrosis Factor - Related Apoptosis-Inducing Ligand (TRAIL) promotes angiogenesis and ischemia- induced neovascularization via NADPH Oxidase 4 (NOX4) and Nitric Oxide - dependent mechanisms

Background — Tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) has the ability to inhibit angiogenesis by inducing endothelial cell death, as well as being able to promote pro- angiogenic activity in vitro. These seemingly opposite effects make its role in ischemic disease unclear. Using Trail_/_ and wildtype mice, we sought to determine the role of TRAIL in angiogenesis and neovascularization following hindlimb ischemia. Methods and Results — Reduced vascularization assessed by real-time 3-dimensional Vevo ultrasound imaging and CD31 staining was evident in Trail_/_ mice after ischemia, and associated with reduced capillary formation and increased apoptosis. Notably, adenoviral TRAIL administration significantly improved limb perfusion, capillary density, and vascular smooth-muscle cell content in both Trail_/_ and wildtype mice. Fibroblast growth factor-2, a potent angiogenic factor, increased TRAIL expression in human microvascular endothelial cell-1, with fibroblast growth factor-2-mediated proliferation, migration, and tubule formation inhibited with TRAIL siRNA. Both fibroblast growth factor-2 and TRAIL significantly increased NADPH oxidase 4 (NOX4) expression. TRAIL-inducible angiogenic activity in vitro was inhibited with siRNAs targeting NOX4, and consistent with this, NOX4 mRNA was reduced in 3-day ischemic hindlimbs of Trail_/_ mice. Furthermore, TRAIL-induced proliferation, migration, and tubule formation was blocked by scavenging H2O2, or by inhibiting nitric oxide synthase activity. Importantly, TRAIL-inducible endothelial nitric oxide synthase phosphorylation at Ser-1177 and intracellular human microvascular endothelial cell-1 cell nitric oxide levels were NOX4 dependent. Conclusions — This is the first report demonstrating that TRAIL can promote angiogenesis following hindlimb ischemia in vivo. The angiogenic effect of TRAIL on human microvascular endothelial cell-1 cells is downstream of fibroblast growth factor-2, involving NOX4 and nitric oxide signaling. These data have significant therapeutic implications, such that TRAIL may improve the angiogenic response to ischemia and increase perfusion recovery in patients with cardiovascular disease and diabetes

Towards the QFT on Curved Spacetime Limit of QGR. I: A General Scheme

In this article and a companion paper we address the question of how one might obtain the semiclassical limit of ordinary matter quantum fields (QFT) propagating on curved spacetimes (CST) from full fledged Quantum General Relativity (QGR), starting from first principles. We stress that we do not claim to have a satisfactory answer to this question, rather our intention is to ignite a discussion by displaying the problems that have to be solved when carrying out such a program. In the present paper we propose a scheme that one might follow in order to arrive at such a limit. We discuss the technical and conceptual problems that arise in doing so and how they can be solved in principle. As to be expected, completely new issues arise due to the fact that QGR is a background independent theory. For instance, fundamentally the notion of a photon involves not only the Maxwell quantum field but also the metric operator - in a sense, there is no photon vacuum state but a "photon vacuum operator"! While in this first paper we focus on conceptual and abstract aspects, for instance the definition of (fundamental) n-particle states (e.g. photons), in the second paper we perform detailed calculations including, among other things, coherent state expectation values and propagation on random lattices. These calculations serve as an illustration of how far one can get with present mathematical techniques. Although they result in detailed predictions for the size of first quantum corrections such as the gamma-ray burst effect, these predictions should not be taken too seriously because a) the calculations are carried out at the kinematical level only and b) while we can classify the amount of freedom in our constructions, the analysis of the physical significance of possible choices has just begun.Comment: LaTeX, 47 p., 3 figure

Lattice knot theory and quantum gravity in the loop representation

We present an implementation of the loop representation of quantum gravity on a square lattice. Instead of starting from a classical lattice theory, quantizing and introducing loops, we proceed backwards, setting up constraints in the lattice loop representation and showing that they have appropriate (singular) continuum limits and algebras. The diffeomorphism constraint reproduces the classical algebra in the continuum and has as solutions lattice analogues of usual knot invariants. We discuss some of the invariants stemming from Chern--Simons theory in the lattice context, including the issue of framing. We also present a regularization of the Hamiltonian constraint. We show that two knot invariants from Chern--Simons theory are annihilated by the Hamiltonian constraint through the use of their skein relations, including intersections. We also discuss the issue of intersections with kinks. This paper is the first step towards setting up the loop representation in a rigorous, computable setting.Comment: 23 pages, RevTeX, 14 figures included with psfi

Consistency Conditions for Fundamentally Discrete Theories

The dynamics of physical theories is usually described by differential equations. Difference equations then appear mainly as an approximation which can be used for a numerical analysis. As such, they have to fulfill certain conditions to ensure that the numerical solutions can reliably be used as approximations to solutions of the differential equation. There are, however, also systems where a difference equation is deemed to be fundamental, mainly in the context of quantum gravity. Since difference equations in general are harder to solve analytically than differential equations, it can be helpful to introduce an approximating differential equation as a continuum approximation. In this paper implications of this change in view point are analyzed to derive the conditions that the difference equation should satisfy. The difference equation in such a situation cannot be chosen freely but must be derived from a fundamental theory. Thus, the conditions for a discrete formulation can be translated into conditions for acceptable quantizations. In the main example, loop quantum cosmology, we show that the conditions are restrictive and serve as a selection criterion among possible quantization choices.Comment: 33 page