Exploratory project 2019 - deep learning for particle-laden viscoelastic flow modelling

[extract] Objetives: explore the possibility of using Deep Learning (DL) techniques to evaluate the drag coefficient of small non-Brownian particles translating and settling in nonlinear viscoelastic fluids. The long-term objective is the development of a 3D numerical code for particle-laden viscoelastic flows (PLVF), which will contribute to understanding many advanced manufacturing and industrial operations, specifically the hydraulic fracturing process

Effects of elasticity, inertia and viscosity ratio on the drag coefficient of a sphere translating through a viscoelastic fluid

The ability to simulate the behavior of dilute suspensions, considering Eulerian-Lagrangian approaches, requires proper drag models, which should be valid for a wide range of process and material parameters. These drag models allow to calculate the momentum exchange between the continuous and dispersed phases. The currently available drag models are only valid for inelastic constitutive fluid models. This work aims at contributing to the development of drag models appropriate for dilute suspensions, where the continuous phase presents viscoelastic characteristics. To this aim, we parametrize the effects of fluid elasticity, namely, the relaxation and retardation times, as well as inertia on the drag coefficient of a sphere translating through a viscoelastic fluid, described by the Oldroyd-B model. To calculate the drag coefficient we resort to three-dimensional direct numerical simulations of unconfined viscoelas tic flows past a stationary sphere, at different Reynolds number, Re, over a wide range of Deborah numbers (< 9), and the polymer viscosity ratios. For low Re (< 1), we identified a non-monotonic trend for the drag coefficient correction (the ratio between the calculated drag coefficient and the one obtained for Stokes-flow). It initially decreases with the increase of De, for low De values (< 1), which is followed by a significant growth, due to the large elastic stresses that are developed on both the surface and wake of the sphere. These behaviors, observed in the inertia less flow regime, are amplified as the polymer viscosity ratio approaches unity. At higher Re (> 1), the drag coefficient correction is found to be always bigger than unity, but smaller than the enhancement calculated in creeping flow limit.The authors would like to acknowledge the funding by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT - Portuguese Foundation for Science and Technology under the projects UID/CTM/50025/2013 and POCI-01-0247-FEDER-017656

A fully-resolved immersed boundary numerical method to simulate particle-laden viscoelastic flows

Fluid-particle transport systems present a significant practical relevance, in several engineering applications, such as oil sands mining and polymer processing. In several cases it is essential to consider that the fluid, in which the particles are dispersed, has underlying viscoelastic characteristics. For this aim, a novel numerical algorithm was implemented on an open-source finite-volume viscoelastic fluid flow solver coupled with an immersed boundary method, by extending the open-source computational fluid dynamics library CFDEMcoupling. The code is able to perform fully-resolved simulations, wherein all flow scales, associated with the particle motion, are resolved. Additionally, the formulation employed exploits the log-conformation tensor approach, to avoid high Weissenberg number issues. The accuracy of the algorithm was evaluated by studying several benchmark flows, namely: (i) the sedimentation of a sphere in a bounded domain; (ii) rotation of a sphere in simple shear flow; (iii) the cross-stream migration of a neutrally buoyant sphere in a steady Poiseuille flow. In each case, a comparison of the results obtained with the newly developed code with data reported in the literature is performed, in order to assess the code accuracy and robustness. Finally, the capability of the code to solve a physical challenging problem is illustrated by studying the interactions and flowinduced alignment of three spheres in a wall-bounded shear flow. The role of the fluid rheology and finite gap size on both the approach rate and pathways of the solid particles are described [1].This work is funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT - Portuguese Foundation for Science and Technology under the project UID/CTM/50025/2013. The authors would like to acknowledge the Minho University cluster under the project Search-ON2: Revitalization of HPC infrastructure of UMinho (NORTE-07-0162-FEDER-000086), co-funded by the North Portugal Regional Operational Programme (ON.2-0 Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF)

Development of a two-way coupled fully resolved immersed boundary method numerical code for particle laden viscoelastic flows

Understanding the behaviour of multiphase flows of solid in viscoelastic fluids is essential in several industrial applications, such as oil sands mining and polymer processing. For this aim, a novel numerical algorithm was implemented on an open-source finite-volume fluid flow solver coupled with an immersed boundary method, to allow the use of viscoelastic constitutive equations on the fluid (continuous) phase. To avoid numerical issues related to high Weissenberg number flows the log-conformation tensor approach can be employed on the newly developed algorithm. The accuracy of the algorithm was evaluated by studying several benchmark flows, namely: (i) the sedimentation of a sphere in a bounded domain surrounded by either Newtonian or viscoelastic fluids; (ii) rotation of a sphere in a homogeneous shear viscoelastic fluid flow; (iii) the cross-stream migration of a neutrally buoyant sphere in a steady Poiseuille flow, considering both Newtonian and viscoelastic suspending fluids. All the results obtained, on the referred case studies, allowed either to replicate the ones available on the published literature, or to describe additional effects promoted by the assumption of viscoelastic behaviour on the continuous phase. To illustrate the potential of the developed code, a newly case study of the shear-induced solid particle alignment in wall-bounded Newtonian and viscoelastic fluids was studied. The role of the fluid rheology and finite gap size on both the approach rate and pathways of the solid particles are described.This work is funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT - Portuguese Foundation for Science and Technology under the project UID/CTM/50025/2013. The authors would like to acknowledge the Minho University cluster under the project Search-ON2: Revitalization of HPC infrastructure of UMinho (NORTE-07-0162-FEDER-000086), co-funded by the North Portugal Regional Operational Programme (ON.2-0 Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF)

Assessing the diversity of sea beet (Beta vulgaris L. ssp. maritima) populations

Beta vulgaris L. subsp. maritima (L.) Arcang., sea beet, is a morphologically and genetically variable species, belonging to beet primary gene -pool. This crop wild relative is a valuable genetic resource for resistance improvement in beets and could play an important role in crop yield sustainability. Eleven Madeiran sea beet populations were characterized using morphological descriptors and genetic markers. Our goal was to evaluate these populations as a potential source of valuable genetic material. Morphological characterization showed a high quantitative variation among populations. Plant height and inflorescence height parameters had the highest influence in the separation of populations. Molecular analysis was performed with polymorphic SSRs to determine genetic variability between populations. Both PCA and PCoA revealed three clusters that separated the populations according to morphological and genetic traits, respectively. This study contributes to the knowledge of sea beet diversity in Madeira’s archipelago and to the perception that the islands' specific environmental conditions influence its genetic variability, making these populations a possible gene source for sugar beet breeding programsinfo:eu-repo/semantics/publishedVersio

Efficient Blockade of Akt signaling is a determinant factor to overcome resistance to Matuzumab

<p>Abstract</p> <p>Background</p> <p>Clinical studies have shown antineoplastic effectiveness of monoclonal antibodies (MAbs) against EGFR for different indications. Several MAbs directed to EGFR were developed recently, such as matuzumab, but there is still lack of information on preclinical data on its combination with chemo-radiation. Thus, the present study intended to examine the molecular pathways triggered by matuzumab alone or associated to chemo-radiotherapy in gynecological cell lines and its impact on cell growth and signaling.</p> <p>Results</p> <p>Combination of matuzumab with radiation and cisplatin did not enhance its cytostatic effects on A431, Caski and C33A cells (high, intermediate and low EGFR expression, respectively) in clonogenic assays, when compared to controls. The lack of effect was mediated by persistent signaling through EGFR due to its impaired degradation. In spite of the fact that matuzumab inhibited phosphorylation of EGFR, it had no effect upon cell viability. To analyze which downstream molecules would be involved in the EGFR signaling in the presence of matuzumab, we have tested it in combination with either PD98059 (MAPK inhibitor), or LY294002 (PI3K inhibitor). Matuzumab exhibited a synergic effect with LY294002, leading to a reduction of Akt phosphorylation that was followed by a decrease in A431 and Caski cells survival. The combination of PD98059 and matuzumab did not show the same effect suggesting that PI3K is an important effector of EGFR signaling in matuzumab-treated cells. Nonetheless, matuzumab induced ADCC in Caski cells, but not in the C33A cell line, suggesting that its potential therapeutic effects <it>in vitro </it>are indeed dependent on EGFR expression.</p> <p>Conclusions</p> <p>Matuzumab combined with chemoradiation did not induce cytotoxic effects on gynecological cancer cell lines <it>in vitro, </it>most likely due to impaired EGFR degradation. However, a combination of matuzumab and PI3K inhibitor synergistically inhibited pAkt and cell survival, suggesting that the use of PI3K/Akt inhibitors could overcome intrinsic resistance to matuzumab <it>in vitro. </it>Altogether, data presented here can pave the way to a rational design of clinical strategies in patients with resistant profile to anti-EGFR inhibitors based on combination therapy.</p

Salmonella enterica Typhimurium FljBA operon Sstability: implications regarding the origin of Salmonella enterica I 4,[5],12:i:-

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORSalmonella enterica subsp enterica serovar 4,5,12:i:- has been responsible for many recent Salmonella outbreaks worldwide. Several studies indicate that this serovar originated from S. enterica subsp enterica serovar Typhimurium, by the loss of the flagellar phase II gene (fljB) and adjacent sequences. However, at least two different clones of S. enterica 4,5,12:i:- exist that differs in the molecular events responsible for fljB deletion. The aim of this study was to test the stability of the fljBA operon responsible for the flagellar phase variation under different growth conditions in order to verify if its deletion is a frequent event that could explain the origin and dissemination of this serovar. In fact, coding sequences for transposons are present near this operon and in some strains, such as S. enterica Typhimurium LT2, the Fels-2 prophage gene is inserted near this operon. The presence of mobile DNA could confer instability to this region. In order to examine this, the cat (chloramphenicol acetyltransferase) gene was inserted adjacent to the fljBA operon so that deletions involving this genomic region could be identified. After growing S. enterica chloramphenicol-resistant strains under different conditions, more than 104 colonies were tested for the loss of chloramphenicol resistance. However, none of the colonies were sensitive to chloramphenicol. These data suggest that the origin of S. enterica serovar 4,5,12:i:- from Typhimurium by fljBA deletion is not a frequent event. The origin and dissemination of 4,5,12:i:- raise several questions about the role of flagellar phase variation in virulence.Salmonella enterica subsp enterica serovar 4,5,12:i:- has been responsible for many recent Salmonella outbreaks worldwide. Several studies indicate that this serovar originated from S. enterica subsp enterica serovar Typhimurium, by the loss of the flagel1441905719065FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR2009/15956-7; 2012/10608-3; 2012/05382-6; 2014/11280-7; 2013/11880-1308955/2012-9; 141629/2012-601P-04520-201

Perda de solo por erosão em decorrência da ação de adubação fosfatada corretiva sobre a estabilidade de agregados.

A aplicação de fósforo em solos brasileiros é uma prática frequente e tal operação pode afetar alguns atributos do solo. Assim, com o objetivo de avaliar os efeitos da fosfatagem na estabilidade de agregados e nas perdas de solo por erosão de um Latossolo Vermelho Acriférrico típico de Lavras (MG), foram aplicados, em duas parcelas de 72 m2, o equivalente a 450 kg ha-1 de P2O5 a lanço mais 180 kg ha-1 de P2O5 em linhas. A estabilidade de agregados foi determinada por ultra-som para classes de 7,93 a 4,76; 4,76 a 2,00; 2,00 a 1,00; 1,00 a 0,50; 0,50 a 0,25 e < 0,25 mm, e por peneiramento úmido para a fração de 7,93 a 4,76 mm. Também foi estimada a perda de solo por erosão com uso do sistema de pinos. A fosfatagem reduziu significativamente a estabilidade de agregados por peneiramento a úmido e por ultra-som para agregados maiores que 1 mm e aumentou as perdas de solo em 2,85 vezes. Agregados maiores que 2 mm apresentaram estabilidade 2,83 vezes menor com fosfatagem, o que pareceu influenciar as perdas de solo. A fosfatagem reduziu o ponto de efeito salino nulo e aumentou a densidade de cargas negativas, o que explicou os resultados obtidos

Análise da pulsatilidade da testosterona em mulheres com ciclos menstruais ovulatórios

OBJECTIVE: To evaluate the pattern of the pulsatile secretion of testosterone in normal menstrual cycle. METHODS: Eight healthy women with ovulatory menstrual cycles were enrolled. Blood samples were collected at ten-minute intervals for six hours, starting between 7 and 8 am, after a ten-hour fasting, in three phases: mid-follicular (Day 7), late follicular (Day 12) and mid-luteal phase (Day 21). Samples were assayed for testosterone, LH and the baseline also for SHBG. RESULTS: Testosterone pulse frequency, mean amplitude pulse, percentage of increment in pulse amplitude, mean duration of pulses and pulse interval were similar in the three phases. LH pulsatility was statistically different among the three phases (p < 0.001) representing normal ovulatory cycles. CONCLUSIONS: These data increase the knowledge about the testosterone secretion profile in the human menstrual cycle and can be used as a contribution to clinical investigation in both hyperandrogenism and androgen insufficiency syndrome.OBJETIVO: Avaliar o padrão pulsátil da secreção da testosterona em mulheres normais. MÉTODOS: Oito mulheres saudáveis com ciclos ovulatórios foram selecionadas. Amostras sanguíneas foram coletadas a cada dez minutos durante seis horas, começando entre 7 e 8 h da manhã, após dez horas de jejum, nas três fases do ciclo menstrual: folicular média (Dia 7), folicular tardia (Dia 12) e lútea (Dia 21). Foram mensurados: testosterona, LH e, no basal, também SHBG. RESULTADOS: A frequência dos pulsos de testosterona, média da amplitude do pulso, porcentagem do incremento da amplitude, duração e intervalos dos pulsos foram similares nas três fases (p > 0,05). A pulsatilidade do LH foi estatisticamente diferente entre as três fases (p < 0,001), caracterizando padrão característico do ciclo ovulatório normal. CONCLUSÕES: Esses dados aumentam o conhecimento sobre o padrão de secreção da testosterona no ciclo menstrual humano e representam uma contribuição para a investigação clínica, tanto no hiperandrogenismo como na síndrome de insuficiência androgênica