Surface Roughness Enhancement of Indirect-SLS Metal Parts by Laser Surface Polishing

Laser polishing by means of shallow surface melting of indirect-SLS metal parts was achieved using high power CO2 and Nd:YAG lasers raster scanned at high speed. This was an effective technique for reducing surface roughness. The fast moving laser beam provides just enough heat energy to cause melting of the surface peaks. The molten mass then flows into the surface valleys by surface tension, gravity and laser pressure, thus diminishing the roughness. Surface roughness Ra data were obtained by profilometry measurements of the polished samples. An analytical model was developed based on the assumption that the surface of an SLS part consists of semi-spherical caps. The model was used to predict the Ra values as a function of laser power, scan speed and precursor powder particle size. The modeled results fit the empirical data within a 15% error.The Laboratory of Freeform Fabrication gratefully acknowledges the support of the Office of Naval Research for funding the project “Surface Engineering for SFF Processes”, Grant Nº: N00014-00-1-0334Mechanical Engineerin

Competition between plant and bacterial cells at the microscale regulates the dynamics of nitrogen acquisition in wheat (Triticum aestivum)

The ability of plants to compete effectively for nitrogen (N) resources is critical to plant survival. However, controversy surrounds the importance of organic and inorganic sources of N in plant nutrition because of our poor ability to visualize and understand processes happening at the root�microbial�soil interface. Using high-resolution nano-scale secondary ion mass spectrometry stable isotope imaging (NanoSIMS-SII), we quantified the fate of 15N over both space and time within the rhizosphere. We pulse-labelled the soil surrounding wheat (Triticum aestivum) roots with either inline image or 15N-glutamate and traced the movement of 15N over 24 h. Imaging revealed that glutamate was rapidly depleted from the rhizosphere and that most 15N was captured by rhizobacteria, leading to very high 15N microbial enrichment. After microbial capture, approximately half of the 15N-glutamate was rapidly mineralized, leading to the excretion of inline image, which became available for plant capture. Roots proved to be poor competitors for 15N-glutamate and took up N mainly as inline image. Spatial mapping of 15N revealed differential patterns of 15N uptake within bacteria and the rapid uptake and redistribution of 15N within roots. In conclusion, we demonstrate the rapid cycling and transformation of N at the soil�root interface and that wheat capture of organic N is low in comparison to inorganic N under the conditions tested

Root exudate carbon mitigates nitrogen loss in a semi-arid soil

AbstractThe need for increased food production to support the growing global population requires more efficient nutrient management and prevention of nitrogen (N) losses from both applied fertiliser and organic matter (OM) decomposition. This is particularly important in semi-arid rainfed cropping soils, where soil water and temperature are the dominant drivers of N cycling rather than agricultural management. Here we used 14C and 15N techniques to examine how peptide/amino acid turnover, gross and net N transformation rates and nitrous oxide (N2O) emissions responded to long-term plant residue additions and/or short-term root exudate additions. Soil was collected from a semi-arid rainfed field trial with one winter crop per year followed by a summer fallow period, where additional inputs of straw/chaff over 10 years had increased total soil organic C (SOC) by 76% compared to no extra additions (control). These field soils were incubated in the laboratory with or without a synthetic root exudate mixture at a range of temperatures reflecting regional field conditions (5–50 °C). Long-term plant residue additions (to build up total soil OM) did not decrease the risk of N loss as defined by the nitrification:immobilisation (N:I) ratio at most temperatures, so was not an effective management tool to control N losses. In comparison, short-term root exudate additions decreased the risk of N loss at all temperatures in both the control and plant residue treatment field soils. Increased net N mineralisation and decreased microbial C use efficiency at temperatures greater than 30 °C resulted in significant ammonium (NH4+) accumulation. Microbial decomposers appeared to use amino acid-C for growth but peptide-C for energy production. Findings indicate that the greatest risk of N loss in these semi-arid soils will occur during rains at the start of the growing season, due to inorganic N accumulation over summer fallow when there are high soil temperatures, occasional significant rainfall events and no growing plants to release root exudates. While most attempts to manipulate the soil N cycle have occurred during the winter cropping period, our findings highlight the need to manage N supply during summer fallow if we are to minimise losses to the environment from semi-arid soils

Leaching of phthalate acid esters from plastic mulch films and their degradation in response to UV irradiation and contrasting soil conditions

Phthalate acid esters (PAEs) are commonly used plastic additives, not chemically bound to the plastic that migrate into surrounding environments, posing a threat to environmental and human health. Dibutyl phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP) are two common PAEs found in agricultural soils, where degradation is attributed to microbial decomposition. Yet the impact of the plastic matrix on PAE degradation rates is poorly understood. Using 14C-labelled DBP and DEHP we show that migration from the plastic matrix into soil represents a key rate limiting step in their bioavailability and subsequent degradation. Incorporating PAEs into plastic film decreased their degradation in soil, DBP (DEHP) from 79% to 21% (9% to <1%), over four months when compared to direct application of PAEs. Mimicking surface soil conditions, we demonstrated that exposure to ultraviolet radiation accelerated PAE mineralisation twofold. Turnover of PAE was promoted by the addition of biosolids, while the presence of plants and other organic residues failed to promote degradation. We conclude that PAEs persist in soil for longer than previously thought due to physical trapping within the plastic matrix, suggesting PAEs released from plastics over very long time periods lead to increasing levels of contamination

Deep-C storage: Biological, chemical and physical strategies to enhance carbon stocks in agricultural subsoils

Due to their substantial volume, subsoils contain more of the total soil carbon (C) pool than topsoils. Much of this C is thousands of years old, suggesting that subsoils offer considerable potential for long-term C sequestration. However, knowledge of subsoil C behaviour and manageability remains incomplete, and subsoil C storage potential has yet to be realised at a large scale, particularly in agricultural systems. A range of biological (e.g. deep-rooting), chemical (e.g. biochar burial) and physical (e.g. deep ploughing) C sequestration strategies have been proposed, but are yet to be assessed. In this review, we identify the main factors that regulate subsoil C cycling and critically evaluate the evidence and mechanistic basis of subsoil strategies designed to promote greater C storage, with particular emphasis on agroecosystems. We assess the barriers and opportunities for the implementation of strategies to enhance subsoil C sequestration and identify 5 key current gaps in scientific understanding. We conclude that subsoils, while highly heterogeneous, are in many cases more suited to long-term C sequestration than topsoils. The proposed strategies may also bring other tangible benefits to cropping systems (e.g. enhanced water holding capacity and nutrient use efficiency). Furthermore, while the subsoil C sequestration strategies we reviewed have large potential, more long-term studies are needed across a diverse range of soils and climates, in conjunction with chronosequence and space-for-time substitutions. Also, it is vital that subsoils are more consistently included in modelled estimations of soil C stocks and C sequestration potential, and that subsoil-explicit C models are developed to specifically reflect subsoil processes. Finally, further mapping of subsoil C is needed in specific regions (e.g. in the Middle East, Eastern Europe, South and Central America, South Asia and Africa). Conducting both immediate and long-term subsoil C studies will fill the knowledge gaps to devise appropriate soil C sequestration strategies and policies to help in the global fight against climate change and decline in soil quality. In conclusion, our evidence-based analysis reveals that subsoils offer an untapped potential to enhance global C storage in terrestrial ecosystems

The JAK inhibitor ruxolitinib reduces inflammation in an ILC3-independent model of innate immune colitis

Innate immunity contributes to the pathogenesis of inflammatory bowel disease (IBD). However, the mechanisms of IBD mediated by innate immunity are incompletely understood and there are limited models of spontaneous innate immune colitis to address this question. Here we describe a new robust model of colitis occurring in the absence of adaptive immunity. RAG1-deficient mice expressing TNFAIP3 in intestinal epithelial cells (TRAG mice) spontaneously developed 100% penetrant, early-onset colitis that was limited to the colon and dependent on intestinal microbes but was not transmissible to co-housed littermates. TRAG colitis was associated with increased mucosal numbers of innate lymphoid cells (ILCs) and depletion of ILC prevented colitis in TRAG mice. ILC depletion also therapeutically reversed established colitis in TRAG mice. The colitis in TRAG mice was not prevented by interbreeding to mice lacking group 3 ILC nor by depletion of TNF. Treatment with the JAK inhibitor ruxolitinib ameliorated colitis in TRAG mice. This new model of colitis, with its predictable onset and colon-specific inflammation, will have direct utility in developing a more complete understanding of innate immune mechanisms that can contribute to colitis and in pre-clinical studies for effects of therapeutic agents on innate immune-mediated IBD

CHANDRA Observations of X-ray Jet Structure on kpc to Mpc Scales

With its exquisite spatial resolution of better than 0.5 arcsecond, the Chandra observatory is uniquely capable of resolving and studying the spatial structure of extragalactic X-ray jets on scales of a few to a few hundred kilo-parsec. Our analyses of four recent Chandra images of quasar jets interpret the X-ray emission as inverse Compton scattering of high energy electrons on the cosmic microwave background. We infer that these jets are in bulk relativistic motion, carrying kinetic powers upwards of 10^46 ergs/s to distances of hundreds of kpc, with very high efficiency.Comment: 4 pages, 3 figures, to be published in the proceedings of the Bologna jet workshop, "The Physics of Relativistic Jets in the CHANDRA and XMM Era.

The Point of Origin of the Radio Radiation from the Unresolved Cores of Radio-Loud Quasars

Locating the exact point of origin of the core radiation in active galactic nuclei (AGN) would represent important progress in our understanding of physical processes in the central engine of these objects. However, due to our inability to resolve the region containing both the central compact object and the jet base, this has so far been difficult. Here, using an analysis in which the lack of resolution does not play a significant role, we demonstrate that it may be impossible even in most radio loud sources for more than a small percentage of the core radiation at radio wavelengths to come from the jet base. We find for 3C279 that $\sim85$ percent of the core flux at 15 GHz must come from a separate, reasonably stable, region that is not part of the jet base, and that then likely radiates at least quasi-isotropically and is centered on the black hole. The long-term stability of this component also suggests that it may originate in a region that extends over many Schwarzschild radii.Comment: 7 pages with 3 figures, accepted for publication in Astrophysics and Space Scienc