Regenerated woody plants influence soil microbial communities in a subtropical forest

10 páginas.- 4 figuras.- 3 tablas.- referencias.- upplementary data to this article can be found online at https://doi. org/10.1016/j.apsoil.2023.104890Forests are critical for supporting multiple ecosystem services such as climate change mitigation. Microbial diversity in soil provides important functions to maintain and regenerate forest ecosystems, and yet a critical knowledge gap remains in identifying the linkage between attributes of regenerated woody plant (RWP) communities and the diversity patterns of soil microbial communities in subtropical plantations. Here, we investigated the changes in soil microbial communities and plant traits in a nine hectare Chinese fir (Cunninghamia lanceolata; CF) plantation to assess how non-planted RWP communities regulate soil bacterial and fungal diversity, and further explore the potential mechanisms that structure their interaction. Our study revealed that soil bacterial richness was positively associated with RWP richness, whereas soil fungal richness was negatively associated with RWP basal area. Meanwhile, RWP richness was positively correlated with ectomycorrhizal (ECM) fungal richness but negatively correlated with the richness of both pathogenic and saprotrophic fungi, suggesting that the RWP-fungal richness relationship was trophic guild-specific. Soil microbial community beta diversity (i.e., dissimilarity in community composition) was strongly coupled with both RWP beta diversity and the heterogeneity of RWP basal area. Our study highlights the importance of community-level RWP plant attributes for the regulation of microbial biodiversity in plantation systems, which should be considered in forest management programs in the future.This work was funded by the National Key Research and Development Program of China (2021YFD2201301 and 2022YFF1303003), the National Natural Science Foundation of China (U22A20612), and the Key Project of Jiangxi Province Natural Science Foundation of China (20224ACB205003).Peer reviewe

An inducible genome editing system for plants

Conditional manipulation of gene expression is a key approach to investigating the primary function of a gene in a biological process. While conditional and cell-type-specific overexpression systems exist for plants, there are currently no systems available to disable a gene completely and conditionally. Here, we present a new tool with which target genes can efficiently and conditionally be knocked out by genome editing at any developmental stage. Target genes can also be knocked out in a cell-type-specific manner. Our tool is easy to construct and will be particularly useful for studying genes having null alleles that are non-viable or show pleiotropic developmental defects.Peer reviewe

Production of tartaric acid using immobilized recominant cis-epoxysuccinate hydrolase

To investigate the expression and immobilization of recombinant cis-epoxysuccinate hydrolase (ESH), and its application in the biological production of l-(+)-tartaric acid.</p

Cytokinins initiate secondary growth in the Arabidopsis root through a set of LBD genes

During primary growth, plant tissues increase their length, and as these tissues mature, they initiate secondary growth to increase thickness.(1) It is not known what activates this transition to secondary growth. Cytokinins are key plant hormones regulating vascular development during both primary and secondary growth. During primary growth of Arabidopsis roots, cytokinins promote procambial cell proliferation(2,3) and vascular patterning together with the hormone auxin.(4-7) In the absence of cytokinins, secondary growth fails to initiate.(8) Enhanced cytokinin levels, in turn, promote secondary growth.(8,9) Despite the importance of cytokinins, little is known about the downstream signaling events in this process. Here, we show that cytokinins and a few downstream LATERAL ORGAN BOUNDARIES DOMAIN (LBD) family of transcription factors are rate limiting components in activating and further promoting secondary growth in Arabidopsis roots. Cytokinins directly activate transcription of two homologous LBD genes, LBD3 and LBD4. Two other homologous LBDs, LBD1 and LBD11, are induced only after prolonged cytokinin treatment. Our genetic studies revealed a two stage mechanism downstream of cytokinin signaling: while LBD3 and LBD4 regulate activation of secondary growth, LBD1, LBD3, LBD4, and LBD11 together promote further radial growth and maintenance of cambial stem cells. LBD overexpression promoted rapid cell growth followed by accelerated cell divisions, thus leading to enhanced secondary growth. Finally, we show that LBDs rapidly inhibit cytokinin signaling. Together, our data suggest that the cambium-promoting LBDs negatively feed back into cytokinin signaling to keep root secondary growth in balance.Peer reviewe

SUPERHYDROPHOBIC SURFACES PREPARED BY PLASMA FLUORINATION OF LOTUS-LEAF-LIKE AMORPHOUS CARBON FILMS

Lotus-leaf-like amorphous carbon (a-C) films were fabricated on glass and Si substrates by a magnetron sputtering system and fluorinated in carbon tetrafluoride plasma. The fluorinated films (a-C:F) had a maximum contact angle (CA) of about 162°, which is much larger than that of the nonfluorinated films (105°). Furthermore, the geometric microstructure and chemical composition of the a-C:F films were investigated by scanning electron microscopy and Fourier transform infrared spectrometry, respectively. The characterization results indicated that the CA on the surfaces of the a-C:F films can be improved remarkably by the plasma-fluorinated process. Such a-C:F films that combine superhydrophobicity with other properties may have many potential applications.Superhydrophobic, amorphous carbon films, wettability, plasma fluorination, scanning electron microscopy

Icariin Alleviates Nonalcoholic Fatty Liver Disease in Polycystic Ovary Syndrome by Improving Liver Fatty Acid Oxidation and Inhibiting Lipid Accumulation

(1) Background: Icariin is the main component of the Chinese herb Epimedium. A number of studies have shown that it alleviates abnormal lipid metabolism. However, it is not clear whether and how icariin can ameliorate hepatic steatosis with polycystic ovary syndrome (PCOS). This study was designed to explore the anti-hepatosteatosis effect of icariin in rats with polycystic ovary syndrome. (2) Methods: Female Sprague Dawley(SD)rats were treated with a high-fat diet and letrozole for 21 days to make nonalcoholic fatty liver disease (NAFLD) in the polycystic ovary syndrome model. Then model rats were treated with icariin (by gavage, once daily) for 28 days. Serum hormones and biochemical variables were determined by ELISA or enzyme. RNA-sequence analysis was used to enrich related target pathways. Then, quantitative Real-time PCR (qRT-PCR) and Western blot were performed to verify target genes and proteins. (3) Results: Icariin treatment reduced excess serum levels of Testosterone (T), Estradiol (E2), Luteinizing hormone (LH), Follicle-stimulating hormone (FSH), LH/FSH ratio, insulin, triglycerides (TG), and aspartate aminotransferase (AST) in high-fat diet (HFD) and letrozole fed rats. Meanwhile, icariin ameliorated HFD and letrozole-induced fatty liver, as evidenced by a reduction in excess triglyceride accumulation, vacuolization, and Oil Red O staining area in the liver of model rats. Results of RNA-sequencing, western blotting, and qRT-PCR analyses indicated that icariin up-regulated fatty acid translocase (CD36), in mitochondria, and peroxisome proliferator-activated receptor α (PPARα) expression, which led to the enhancement of fatty acid oxidation molecules, such as cytochrome P450, family 4, subfamily a, polypeptide 3 (CYP4A3), carnitine palmitoyltransferase 1 α (CPT1α), acyl-CoA oxidase 1 (ACOX1), medium-chain acyl-CoA dehydrogenase (MCAD), and long-chain acyl-CoA dehydrogenase (LCAD). Besides, icariin reduced lipid synthesis, which elicited stearoyl-Coenzyme A desaturase 1 (SCD1), fatty acid synthase (FASN), and acetyl-CoA (ACC). (4) Conclusion: Icariin showed an ameliorative effect on hepatic steatosis induced by HFD and letrozole, which was associated with improved fatty acid oxidation and reduced lipid accumulation in the liver

Effects of Second Phases on Microstructure, Microhardness, and Corrosion Behavior of Mg-3Sn-(1Ca) Alloys

The effects of second phases on microstructure, microhardness, and corrosion behavior of aged Mg-3Sn (T3) and Mg-3Sn-1Ca (TX31) alloys are investigated systematically. The thermal stability of the CaMgSn phase is higher than that of the Mg2Sn phase, and the microstructure remains essentially unchanged in the TX31 alloy after solution treatment for 28 h at 733 K. The T3 alloy exhibits double age-hardening peaks; one is 54.9 &plusmn; 2.1 HV for 7 h, and the other is 57.4 &plusmn; 2.8 HV for 15 h. However, the microhardness quickly reaches a stable value with increasing aging times in the TX31 alloy due to the no change in CaMgSn phases. It was also found by electrochemical impedance spectra that the corrosion resistance of aged T3 alloy is superior to that of aged TX31 alloy, especially T3 alloy aged for 7 h. The corrosion film of aged T3 alloy is denser, which attributes to most of dissolved Sn in the &alpha;-Mg matrix and the formation of a small quantity of tiny Mg2Sn particles, and effectively prevents the occurrence of further corrosion of the Mg matrix. However, galvanic cells formed between &alpha;-Mg and CaMgSn phases accelerate the corrosion of aged TX31 alloy

Short-Term Starvation Weakens the Efficacy of Cell Cycle Specific Chemotherapy Drugs through G1 Arrest

Short-term starvation (STS) during chemotherapy can block the nutrient supply to tumors and make tumor cells much more sensitive to chemotherapeutic drugs than normal cells. However, because of the diversity of starvation methods and the heterogeneity of tumors, this method’s specific effects and mechanisms for chemotherapy are still poorly understood. In this study, we used HeLa cells as a model for short-term starvation and etoposide (ETO) combined treatment, and we also mimicked the short-term starvation effect by knocking down the glycolytic enzyme GAPDH to explore the exact molecular mechanism. In addition, our study demonstrated that short-term starvation protects cancer cells against the chemotherapeutic agent ETO by reducing DNA damage and apoptosis due to the STS-induced cell cycle G1 phase block and S phase reduction, thereby diminishing the effect of ETO. Furthermore, these results suggest that starvation therapy in combination with cell cycle-specific chemotherapeutic agents must be carefully considered