Chromosomal Organization and Sequence Diversity of Genes Encoding Lachrymatory Factor Synthase in Allium cepa L.

Lachrymatory factor synthase (LFS) catalyzes the formation of lachrymatory factor, one of the most distinctive traits of bulb onion (Allium cepa L.). Therefore, we used LFS as a model for a functional gene in a huge genome, and we examined the chromosomal organization of LFS in A. cepa by multiple approaches. The first-level analysis completed the chromosomal assignment of LFS gene to chromosome 5 of A. cepa via the use of a complete set of A. fistulosum–shallot (A. cepa L. Aggregatum group) monosomic addition lines. Subsequent use of an F2 mapping population from the interspecific cross A. cepa × A. roylei confirmed the assignment of an LFS locus to this chromosome. Sequence comparison of two BAC clones bearing LFS genes, LFS amplicons from diverse germplasm, and expressed sequences from a doubled haploid line revealed variation consistent with duplicated LFS genes. Furthermore, the BAC-FISH study using the two BAC clones as a probe showed that LFS genes are localized in the proximal region of the long arm of the chromosome. These results suggested that LFS in A. cepa is transcribed from at least two loci and that they are localized on chromosome 5

Mycobacterium caprae Infection in Captive Borneo Elephant, Japan

In 2016, disseminated tuberculosis caused by Mycobacterium caprae was diagnosed in a captive Borneo elephant in Japan. The bacterium was initially identified from clinical isolates. An isolate collected during a relapse showed isoniazid monoresistance and a codon 315 katG mutation

Realization of high Ti plasmas and confinement characteristics of ITB plasmas in the LHD deuterium experiments

The deuterium (D) operation was initiated in the LHD in 2017. In the first campaign of the D experiments, we successfully extended the high temperature regime in the LHD. The new record of the ion temperature (Ti) of 10 keV associated with the ion internal transport barrier (ITB) was achieved due to several operational optimization. The thermal confinement characteristics of ITB plasmas were compared between hydrogen and D discharges. The effective ion thermal diffusivity of the ion-ITB plasmas was found to be smaller in the D discharges than that in the H discharges. The profiles of the Ti, the electron density, and the impurity of the high Ti plasmas strongly depended on the magnetic configuration and these profiles tended to peaked in the inward-shifted configuration. It was also found that the electron thermal confinement of the electron-ITB plasmas was clearly improved in the deuterium case. The GKV simulation showed the linear growth rate of TEM/ITG reduced in the plasmas with D both for the ion ITB and the electron ITB plasmas and qualitatively agreed with the tendency of the change in the thermal diffusivity obtained from the power balance analysis

Plasmonically enhanced Faraday effect in metal and ferrite nanoparticles composite precipitated inside glass

Using femtosecond laser irradiation and subsequent annealing, nanocomposite structures composed of spinel-type ferrimagnetic nanoparticles (NPs) and plasmonic metallic NPs have been formed space-selectively within glass doped with both α-Fe2O3 and Al. The Faraday rotation spectra exhibit a distinct negative peak at around 400 nm, suggesting that the ferrimagnetic Faraday response is enhanced by the localized surface plasmon resonance (LSPR) due to metallic Al NPs. At the interfaces in the nanocomposites, the ferrimagnetism of magnetite NPs is directly coupled with the plasmon in the Al NPs. The control of the resonance wavelength of the magneto-optical peaks, namely, the size of plasmonic NPs has been demonstrated by changing the irradiation or annealing conditions