35 research outputs found
Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia
Integrated Jingmenvirus Polymerase Gene in Ixodes ricinus Genome
Members of the jingmenviruses group have been found in arthropods and mammals on all continents except Australia and Antarctica. Two viruses of this group were isolated from patients with fever after a tick bite. Using a nested RT-PCR assay targeting a jingmenvirus polymerase gene fragment, we screened ticks collected in seven regions of Russia and found that the abundant jingmenvirus-positive were of Ixodes ricinus species, with the prevalence ranging from 19.8% to 34.3%. In all cases, DNase/RNase treatment suggested that the detected molecule was DNA and subsequent next generation sequencing (NGS) proved that the viral polymerase gene was integrated in the I. ricinus genome. The copy number of the integrated polymerase gene was quantified by qPCR relative to the ITS2 gene and estimated as 1.32 copies per cell. At least three different genetic variants of the integrated polymerase gene were found in the territory of Russia. Phylogenetic analysis of the integrated jingmenvirus polymerase gene showed the highest similarity with the sequence of the correspondent gene obtained in Serbia from I. ricinus
MELTING RATE CALCULATION ON PREHEATED WIRES OF VARIOUS CHEMISTRY UNDER ARC WELDING
The empirical factors dependences on the process parameters for the welding wires of various chemist ry are o btained for t he empirical formula permitting to calculate the wire melting rate (feed) in a wide range of current s, preheat temperatures, electrode diameters , and stick - outs under the welding in carbon dioxide on the reversed p olarity current. The calculation accuracy by the empirical formula and by the known computational melting model of the same wires is comparatively appraised. The effect of stick - out and preheat temperature increase of the welding wires on the productivity - boosting features of their melting is asse ssed
INITIAL ARC IGNITION CONTROL IN GAS-SHIELDED WELDING
The control method of the initial arc ignition in the gas-shielded welding is offered. It is based on the short-circuit current limitation after heating the wire stick-out with the metal passing current to the plastic state for the time of the wire burnout approach point at the stick-out-to-work distance not exceeding the arc breaking length. The dependences describing the conditions necessary and sufficient for the reliable establishment of the welding process after the first wire-work contact are experimentally investigated and analytically determined
Alphanumerical Visual Display Made Of Dna Logic Gates For Drug Susceptibility Testing Of Pathogens
Molecular diagnostics of drug-resistant pathogens require the analysis of point mutations in bacterial or viral genomes, which is usually performed by trained professionals and/or by sophisticated computer algorithms. We have developed a DNA-based logic system that autonomously analyzes mutations found in the genome of Mycobacterium tuberculosis complex (MTC) bacteria and communicates the output to a human user as alphanumeric characters read by the naked eye. The five-gate system displays “O” (“no infection”) for the absence of MTC infection and “P” or “F” for passing or failing a drug-susceptibility test, respectively
Profiling of 179 miRNA expression in blood plasma of lung cancer patients and cancer-free individuals
Lung cancer is one of major cancers, and survival of lung cancer patients is dictated by the timely detection and diagnosis. Cell-free circulating miRNAs were proposed as candidate biomarkers for lung cancer. These RNAs are frequently deregulated in lung cancer and can persist in bodily fluids for extended periods of time, shielded from degradation by membrane vesicles and biopolymer complexes. To date, several groups reported the presence of lung tumour-specific subsets of miRNAs in blood. Here we describe the profiling of blood plasma miRNAs in lung cancer patients, healthy individuals and endobronchitis patients using miRCURY LNA miRNA qPCR Serum/Plasma Panel (Exiqon). From 241 ratios differently expressed between cancer patients and healthy individuals 19 miRNAs were selected for verification using the same platform. LASSO-penalized logistic regression model, including 10 miRNA ratios comprised of 14 individual miRNAs discriminated lung cancer patients from both control groups with AUC of 0.979
Comparative Study of Extracellular Vesicles from the Urine of Healthy Individuals and Prostate Cancer Patients.
Recent studies suggest that extracellular vesicles may be the key to timely diagnosis and monitoring of genito-urological malignancies. In this study we investigated the composition and content of extracellular vesicles found in the urine of healthy donors and prostate cancer patients. Urine of 14 PCa patients and 20 healthy volunteers was clarified by low-speed centrifugation and total extracellular vesicles fraction was obtain by high-speed centrifugation. The exosome-enriched fraction was obtained by filtration of total extracellular vesicles through a 0.1 μm pore filter. Transmission electron microscopy showed that cell-free urine in both groups contained vesicles from 20 to 230 nm. Immunogold staining after ultrafiltration demonstrated that 95% and 90% of extracellular vesicles in healthy individuals and cancer patients, respectively, were exosomes. Protein, DNA and RNA concentrations as well as size distribution of extracellular vesicles in both fractions were analyzed. Only 75% of the total protein content of extracellular vesicles was associated with exosomes which amounted to 90-95% of all vesicles. Median DNA concentrations in total extracellular vesicles and exosome-enriched fractions were 18 pg/ml and 2.6 pg/ml urine, correspondingly. Urine extracellular vesicles carried a population of RNA molecules 25 nt to 200 nt in concentration of no more than 290 pg/ml of urine. Additionally, concentrations of miR-19b, miR-25, miR-125b, and miR-205 were quantified by qRT-PCR. MiRNAs were shown to be differently distributed between different fractions of extracellular vesicles. Detection of miR-19b versus miR-16 in total vesicles and exosome-enriched fractions achieved 100%/93% and 95%/79% specificity/sensitivity in distinguishing cancer patients from healthy individuals, respectively, demonstrating the diagnostic value of urine extracellular vesicles
Plasma miR-19b and miR-183 as Potential Biomarkers of Lung Cancer
<div><p>Lung cancer is a complex disease that often manifests at the point when treatment is not effective. Introduction of blood-based complementary diagnostics using molecular markers may enhance early detection of this disease and help reduce the burden of lung cancer. Here we evaluated the diagnostic potential of seven plasma miRNA biomarkers (miR-21, -19b, -126, -25, -205, -183, -125b) by quantitative reverse transcription PCR. Influence clinical and demographical characteristics, including age, tumor stage and cancer subtype on miRNA levels was investigated. Four miRNAs were significantly dysregulated (miR-19b, -21, -25, -183) in lung cancer patients. Combination of miR-19b and miR-183 provided detection of lung cancer with 94.7% sensitivity and 95.2% specificity (AUC = 0.990). Thus, miRNAs have shown the potential to discriminate histological subtypes of lung cancer and reliably distinguish lung cancer patients from healthy individuals.</p></div
ROC analysis of miRNA expression.
<p>Receiving Operator Characteristic (ROC) curves for individual miRNAs and combination of miR-19b and miR-183. (A)–(E) Individual miRNAs; (F) Binary logistic regression of miR-19b and miR-183. ROC curves discriminate squamous cell carcinoma (SCC), adenocarcinoma (AD) and total study population of lung cancer patients (LC) from healthy individuals (HD).</p
Targets and functions of selected miRNAs.
<p>Targets and functions of selected miRNAs.</p