Études cinétiques par HPLC et purification de la y-glutamyltranspeptidase recombinante humaine provenant des levures

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal

Distinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization.

The V3 loop of the HIV-1 envelope (Env) protein elicits a vigorous, but largely non-neutralizing antibody response directed to the V3-crown, whereas rare broadly neutralizing antibodies (bnAbs) target the V3-base. Challenging this view, we present V3-crown directed broadly neutralizing Designed Ankyrin Repeat Proteins (bnDs) matching the breadth of V3-base bnAbs. While most bnAbs target prefusion Env, V3-crown bnDs bind open Env conformations triggered by CD4 engagement. BnDs achieve breadth by focusing on highly conserved residues that are accessible in two distinct V3 conformations, one of which resembles CCR5-bound V3. We further show that these V3-crown conformations can, in principle, be attacked by antibodies. Supporting this conclusion, analysis of antibody binding activity in the Swiss 4.5 K HIV-1 cohort (n = 4,281) revealed a co-evolution of V3-crown reactivities and neutralization breadth. Our results indicate a role of V3-crown responses and its conformational preferences in bnAb development to be considered in preventive and therapeutic approaches

Le syndrome de Marfan (thérapeutiques actuelles et d'avenir pour la prise en charge de la dilatation aortique)

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Lignocellulosic fibres surface interactions in enzymatic reaction using data-mining

peer reviewedLignocellulosic fibres modification focused so far essentially on the resulting material properties to create functional fibres instead of determining the reaction influencing parameters. Using a data-mining algorithm, surface chemical composition of the fibres after modifications was compared to multiple signals. A 24 h reaction at either 25 °C or 60 °C, pH5 was conducted in presence of trans-ferulic acid, laccase, and lignocellulosic fibres (flax, hemp, or cellulose) having different chemical surface composition. Dimers and trimers were detected in variable concentrations in the reaction filtrate and extractive. At 25 °C, crystalline cellulose, amorphous cellulose, xylans, mannans, and lignins were well correlated to specific reaction products while at 60 °C, only lignins and xylan were found correlated to reaction products. Fibres surface composition affected the extractive profile. Lignocellulosic surface composition influence on the product formed was unveiled using a data mining approach. This study presents a way to unveil non-evident chemical interface interaction in reactions