Basic Cardiopulmonary Resuscitation Manual

The purpose of this publication is to systematize the knowledge necessary to make Cardiopulmonary Resuscitation (CPR) or Cardiorespiratory Resuscitation (CRR) known to nursing health specialists, which translates as a set of temporary and internationally standardized maneuvers assigned to ensure the oxygenation of the vital organs when the blood circulation of an individual stops suddenly, independently of the cause of the Cardiorespiratory arrest (CRA). This knowledge is found correctly delimited in The Basic Cardiopulmonary Resuscitation Manual, which especially emphasizes the step-by-step methodology and techniques to follow for Cardiopulmonary Resuscitation (CPR). This is a basic work that cannot be overlooked by health care specialists, the society, and the public in general, because of the high probability that this situation will present at some moment in life. Thus, it is recommended to be prepared to help some person in an emergency situation. The manual is an excellent consultation work for health professionals. Given that what is expressed in this work is fundamental for life, and its objective is to make known the relevant information concerning the knowledge of CPR

Molecular Events behind the Selectivity and Inactivation Properties of Model NaK-Derived Ion Channels

Y55W mutants of non-selective NaK and partly K+-selective NaK2K channels have been used to explore the conformational dynamics at the pore region of these channels as they interact with either Na+ or K+. A major conclusion is that these channels exhibit a remarkable pore conformational flexibility. Homo-FRET measurements reveal a large change in W55–W55 intersubunit distances, enabling the selectivity filter (SF) to admit different species, thus, favoring poor or no selectivity. Depending on the cation, these channels exhibit wide-open conformations of the SF in Na+, or tight induced-fit conformations in K+, most favored in the four binding sites containing NaK2K channels. Such conformational flexibility seems to arise from an altered pattern of restricting interactions between the SF and the protein scaffold behind it. Additionally, binding experiments provide clues to explain such poor selectivity. Compared to the K+-selective KcsA channel, these channels lack a high affinity K+ binding component and do not collapse in Na+. Thus, they cannot properly select K+ over competing cations, nor reject Na+ by collapsing, as K+-selective channels do. Finally, these channels do not show C-type inactivation, likely because their submillimolar K+ binding affinities prevent an efficient K+ loss from their SF, thus favoring permanently open channel states.This work was partly supported by grants PGC2018-093505-B-I00 from the Spanish “Ministerio de Ciencia e Innovación”/FEDER, UE, and FCT-Fundação para a Ciência e a Tecnologia, I.P., under the scope of the project UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute for Bioengineering and Biosciences—iBB and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB

Scavenger assemblages are structured by complex competition and facilitation processes among vultures

Understanding the factors that allow multiple species to coexist and share resources is an outstanding question in community ecology. Animals that share resources tend to use different strategies to decrease potential competition, through morphological adaptations, establishment of hierarchies, behavioral adaptations or spatial or temporal segregation. The main objective of this study was to infer interspecific processes of competition and facilitation through the study of species co-occurrence patterns in a vertebrate scavenger guild in de Brazilian cerrado. We analyzed patterns of spatial and temporal co-occurrence between species pairs, both qualitatively and quantitatively, and determined the activity patterns of the different scavenger species. For this purpose, we placed and monitored 11 large (i.e. goat) and 45 small (i.e. chicken) carcasses by camera-trapping, obtaining a total of 27 448 images. Our results show complex competitive and facilitative relationships among scavenging species in the Brazilian cerrado that are influenced by carcass size and change depending on the spatial and temporal scale at which they are analyzed. The scavenger assemblages that consumed large and small carcasses were different, evidencing resource partitioning between obligate and facultative scavengers. Furthermore, as an alternative to reduce competition levels, most species showed differences in their scavenging patterns, in addition to a strong temporal segregation during carcass consumption. Regarding New World vultures, our results suggest a strong interference competition between species with clear differences in their ecological traits (e.g. size, social behavior). However, we also found evidence of facilitation processes between vulture species in the location and access to the interior of the carcasses. Our findings highlight the role of obligate scavengers both in competition and facilitation processes in this vertebrate scavenger community. Future research should focus on investigating which species play the most important role in the structure and dynamics of this community, also considering intraspecific and behavioral patterns.LNA, ZMR and ESG were supported by the Generalitat Valenciana and the European Social Fund (ACIF/2019/056, APOSTD/2019/016, SEJI/2018/024, respectively), and JASZ by funds from the Spanish Ministry of Science, Innovation and Universities and the European Regional Development Fund (RTI2018-099609-B-C21). ESG received the grant RYC2019-027216-I funded by MCIN/AEI/10.13039/501100011033 and by ESF Investing in your future. ZMR was also supported by a postdoctoral contract funded by the Junta de Andalucía (POSTDOC_21_00353)

Olfactory dysfunction in young smokers J.

To establish the prevalence of olfactory dysfunction in smoking and non-smoking students of our Faculty who attend the Department of Otolaryngology (ENT) of our Hospital. Materials and method: Students (smokers and non-smokers) that do and do not suffer from olfactory dysfunction. We applied a questionnaire and a pocket smell test for screening all of the students. Results: We evaluated 207 students, between 18 and 30 years old; 50.7% (n=105) were women and 49.3% (n=102) were men. The smokers among them smoked up to 6 packs per year. One hundred twenty three students were non-smokers and 84 students were smokers. Of the 84 students who were smokers, 67 (79.7%) answered the Pocket Smell Test correctly (3/3) and 17 (20.2%) students had one or more errors. We had 123 non-smoker students and 103 (83.7%) students answered the Pocket Smell Test correctly and 20 (16.2%) answered with one or more errors. The prevalence of olfactory dysfunction in young smokers with a 95% conidence interval would be 32.8%. Conclusions: This study informed us about olfactory dysfunctions in our student population and their smoking habits. We corroborate that the Pocket Smell Test is reliable with the questionnaire; nevertheless it is a screening test. We have a population of young people who smoke one cigarette per day and who didn’t have a signiicant alteration in their ability of smell at the time of the study. This is consistent with medical literature. More studies should be conducted in order to expand this information

Nuevo método para acelerar la curación de la enfermedad de Perthes

Se presenta el caso de un paciente de 13 años de edad, con el diagnóstico de enfermedad de Perthes avanzada, atendido en el servicio de Ortopedia y Traumatología del Hospital Universitario Celia Sánchez Manduley, en el que se empleó por primera vez el tratamiento quirúrgico convencional asociado al empleo de la oxigenación hiperbárica. La evolución del paciente fue satisfactoria, la nueva combinación de tratamientos, logró reducir el período de evolución de la enfermedad en seis meses.A case of 13 years old patient with advanced Perthes disease is presented. The child was admitted to the orthopedics and traumathology service of the University Hospital Celia Sánchez Manduley. Surgical treatment was associated to hiperbaric oxynation therapy. The patient's outcome was satisfactory. The new treatment combination was able to reduce the evolution's period of disease in six months

Ganglion-specific splicing of TRPV1 underlies infrared sensation in vampire bats.

Vampire bats (Desmodus rotundus) are obligate blood feeders that have evolved specialized systems to suit their sanguinary lifestyle. Chief among such adaptations is the ability to detect infrared radiation as a means of locating hotspots on warm-blooded prey. Among vertebrates, only vampire bats, boas, pythons and pit vipers are capable of detecting infrared radiation. In each case, infrared signals are detected by trigeminal nerve fibres that innervate specialized pit organs on the animal's face. Thus, vampire bats and snakes have taken thermosensation to the extreme by developing specialized systems for detecting infrared radiation. As such, these creatures provide a window into the molecular and genetic mechanisms underlying evolutionary tuning of thermoreceptors in a species-specific or cell-type-specific manner. Previously, we have shown that snakes co-opt a non-heat-sensitive channel, vertebrate TRPA1 (transient receptor potential cation channel A1), to produce an infrared detector. Here we show that vampire bats tune a channel that is already heat-sensitive, TRPV1, by lowering its thermal activation threshold to about 30 °C. This is achieved through alternative splicing of TRPV1 transcripts to produce a channel with a truncated carboxy-terminal cytoplasmic domain. These splicing events occur exclusively in trigeminal ganglia, and not in dorsal root ganglia, thereby maintaining a role for TRPV1 as a detector of noxious heat in somatic afferents. This reflects a unique organization of the bat Trpv1 gene that we show to be characteristic of Laurasiatheria mammals (cows, dogs and moles), supporting a close phylogenetic relationship with bats. These findings reveal a novel molecular mechanism for physiological tuning of thermosensory nerve fibres

Recovery of Beach-Foredune Vegetation after Disturbance by Storms

Beaches and foredunes are characterized by being exposed to harsh environmental restrictions mainly due to salt spray, burial by sand and occasional storms. In response to this, plant species from these habitats have adaptations that allow them not only to survive in these environments, but also to recover after the impact of disturbances such as severe storms. In this study, we had the opportunity to study vegetation recovery on the coast of Huelva, Spain, after the impact of a strong winter storm in 2017 which severely affected the vegetation growing on the beach and foredune. Species composition and abundance of vegetation was compared before (2013) and after (2018) the storm hit the coast in 2017. The results show that the effects of the storm were still evident a year later. Native species, mainly perennials, were able to recover almost completely to predisturbance levels. In contrast, the invasive species, Oenothera drummondii, which was abundant before the storm, disappeared from the beach and its presence in the foredune was greatly reduced. Given the forecasts of sea level rise and the increased frequency and intensity of storms, it is necessary to sustain and reinforce the natural coastal sectors where native plant communities maintain the resilience of coastal ecosystems when impacted to these disturbances.Consejo Nacional de Ciencia y Tecnología (CONACYT) 7799Ministerio de Economía y Competitividad CGL2015-65058-

In vivo extracellular matrix protein expression by human periodontal ligament after stimulation with orthodontic force

It is well known that the orthodontic force applied to teeth generates a series of events that remodel the periodontal ligament (PDL). Extracellular matrix proteins (ECM) are described as molecular regulators of these events. However, the exact contribution of these proteins in human PDL modeling by orthodontic force application in vivo is not known. The aim of this present study was to evaluate the protein expression of fibronectin, laminin and vitronectin by human PDL from teeth on which orthodontic force was applied. Twenty healthy individuals were included in the study. PDL was obtained from teeth after a 3-week treatment with orthodontic force. PDL-protein samples were separated on 7.5% SDS-PAGE Western blot analysis with specific monoclonal antibodies for fibronectin, laminin and vitronectin. Bands were visualized with an enhanced chemiluminescence detection system and densitometric. Scanning of bands was carried out to compare differences in protein expression. A significant increment in fibronectin (13.9%), laminin (16.5%) and vitronectin (14.2%) expression was found in PDL from teeth treated with orthodontic force for 3 weeks in comparison with teeth in the control group. Our results support the concept that molecular changes take place by application of orthodontic forces to the PDL. Over expression of these proteins suggests that extracellular matrix (ECM) remodeling could be generated in response to mechanical stress.Keywords: Extracellular matrix proteins, periodontal ligament, orthodontic forceAfrican Journal of Biotechnology Vol. 9(34), pp. 5599-5604, 23 August, 201