541 research outputs found
Motion of nanodroplets near edges and wedges
Nanodroplets residing near wedges or edges of solid substrates exhibit a
disjoining pressure induced dynamics. Our nanoscale hydrodynamic calculations
reveal that non-volatile droplets are attracted or repelled from edges or
wedges depending on details of the corresponding laterally varying disjoining
pressure generated, e.g., by a possible surface coating.Comment: 12 pages, 7 figure
Comparative study on some physiologic, biometrics, nutritional value and molecular characteristics of Mighan Lake’s Artemia (Arak)
Due to the importance of identifying the major characteristics of Artemia populations, in this study some physiologic, biometric, nutritional and genetic characteristics of one Artemia population from Iran named Arak’s Artemia (Mighan Lake) was studied. The hatched larvae of Artemia were reared in the saline water of 80 g/l with standards method in which percentage of survival and growth were evaluated on days 3, 7, 11 and 15 of culture period. In order to study the morphometric characteristics of Artemia, diameter of full cysts as well as 11 more morphological parameters of adult Artemia were measured. The fatty acid profile was analyzed by gas chromatography. The Genetic characteristics were compared with other Artemia populations by sequencing after PCR amplification of Hsp 26 gene. According to the results, the diameter of cysts and nauplii instar were 276.28 and 544.66 micron, respectively. The growth and survival of brine shrimp Artemia, in comparison with other populations, reflected good growth and survival of this population. The results of fatty acids profile also showed higher amounts of polyunsaturated fatty acids in this Artemia compared to other populations cultured under identical conditions. The morphometric characteristics and genetic study of Hsp 26 gene showed great affinity of this population with the parthenogenetic brine shrimp Artemia. However, individual differences could be used to characterize this population
Recommended from our members
Biporous Metal-Organic Framework with Tunable CO2/CH4 Separation Performance Facilitated by Intrinsic Flexibility.
In this work, we report the synthesis of SION-8, a novel metal-organic framework (MOF) based on Ca(II) and a tetracarboxylate ligand TBAPy4- endowed with two chemically distinct types of pores characterized by their hydrophobic and hydrophilic properties. By altering the activation conditions, we gained access to two bulk materials: the fully activated SION-8F and the partially activated SION-8P with exclusively the hydrophobic pores activated. SION-8P shows high affinity for both CO2 ( Qst = 28.4 kJ/mol) and CH4 ( Qst = 21.4 kJ/mol), while upon full activation, the difference in affinity for CO2 ( Qst = 23.4 kJ/mol) and CH4 ( Qst = 16.0 kJ/mol) is more pronounced. The intrinsic flexibility of both materials results in complex adsorption behavior and greater adsorption of gas molecules than if the materials were rigid. Their CO2/CH4 separation performance was tested in fixed-bed breakthrough experiments using binary gas mixtures of different compositions and rationalized in terms of molecular interactions. SION-8F showed a 40-160% increase (depending on the temperature and the gas mixture composition probed) of the CO2/CH4 dynamic breakthrough selectivity compared to SION-8P, demonstrating the possibility to rationally tune the separation performance of a single MOF by manipulating the stepwise activation made possible by the MOF's biporous nature
Thermal inactivation and conformational lock studies on glucose oxidase
In this study, the dissociative thermal inactivation
and conformational lock theories are applied for the
homodimeric enzyme glucose oxidase (GOD) in order to
analyze its structure. For this purpose, the rate of activity
reduction of glucose oxidase is studied at various temperatures
using b-D-glucose as the substrate by incubation of
enzyme at various temperatures in the wide range between
40 and 70 �C using UV–Vis spectrophotometry. It was
observed that in the two ranges of temperatures, the
enzyme has two different forms. In relatively low temperatures,
the enzyme is in its dimeric state and has normal
activity. In high temperatures, the activity almost disappears
and it aggregates. The above achievements are confirmed
by dynamic light scattering. The experimental
parameter ‘‘n’’ as the obvious number of conformational
locks at the dimer interface of glucose oxidase is obtained
by kinetic data, and the value is near to two. To confirm the
above results, the X-ray crystallography structure of the
enzyme, GOD (pdb, 1gal), was also studied. The secondary
and tertiary structures of the enzyme to track the thermal
inactivation were studied by circular dichroism and
fluorescence spectroscopy, respectively. We proposed a
mechanism model for thermal inactivation of GOD based
on the absence of the monomeric form of the enzyme by
circular dichroism and fluorescence spectroscopy
The correlation of RNase A enzymatic activity with the changes in the distance between Nepsilon2-His12 and N delta1-His119 upon addition of stabilizing and destabilizing salts.
The effect of stabilizing and destabilizing salts on the catalytic behavior of ribonuclease A (RNase A) was investigated at pH 7.5 and 25 degrees C, using spectrophotometric, viscometric and molecular dynamic methods. The changes in the distance between N(epsilon2) of His(12) and N(delta1) of His(119) at the catalytic center of RNase A upon the addition of sodium sulfate, sodium hydrogen sulfate and sodium thiocyanate were evaluated by molecular dynamic methods. The compactness and expansion in terms of Stokes radius of RNase A upon the addition of sulfate ions as kosmotropic salts, and thiocyanate ion as a chaotropic salt, were estimated by viscometric measurements. Enzyme activity was measured using cytidine 2', 3'-cyclic monophosphate as a substrate. The results from the measurements of distances between N(epsilon2) of His(12) and N(delta1) of His(119) and Stokes radius suggest (i) that the presence of sulfate ions decreases the distance between the catalytic His residues and increases the globular compactness, and (ii) that there is an expansion of the enzyme surface as well as elongation of the catalytic center in the presence of thiocyanate ion. These findings are in agreement with activity measurements
Thermodynamic approach to holographic dark energy and the R\'{e}nyi entropy
Using the first law of thermodynamics, we propose a relation between the
system entropy () and its IR () and UV () cutoffs. In addition,
applying this relation to the apparent horizon of flat FRW universe, whose
entropy meets the R\'{e}nyi entropy, a new holographic dark energy model is
addressed. Thereinafter, the evolution of the flat FRW universe, filled by a
pressureless source and the obtained dark energy candidate, is studied. In our
model, there is no mutual interaction between the cosmos sectors. We find out
that the obtained model is theoretically powerful to explain the current
accelerated phase of the universe. This result emphasizes that the generalized
entropy formalism is suitable for describing systems including the long-range
interactions such as gravity.Comment: Accepted by Eur. Phys. J. C (2018
- …