Effect of orthodontic treatment on saliva, plaque and the levels of Streptococcus mutans and Lactobacillus

Objectives: The aim of this study was to identify changes in the oral environment with clinical, salivary and bacterial risk markers after placement of fixed orthodontic appliances on permanent dentition. Material and Methods: With ethical approval, we used different techniques to analyzed clinical, salivary and bacterial risk markers in 34 patients (mean age, 16.7 ± 5.2 years), 14 males and 20 females; before starting orthodontic treatment and 1 month after. Clinical risk markers (decayed, missing, and filled surfaces [DMFS], O?Leary´s plaque index, and plaque pH); salivary markers (unstimulated and stimulated saliva flow rate, buffer capacity, pH, and occult blood in saliva) and bacterial counts (Streptococcus mutans and Lactobacillus). Data were analyzed by paired t-test and ?2 test. Results: This study showed that orthodontic appliances increased the stimulated salivary flow rate (p=0.0001), buffer capacity (p=0.0359), salivary pH (p=0.0246) and occult blood in saliva (p=0.0305). Bacterial levels increased slightly after 1 month of treatment, without statistical significance. Between genders, initially we observed differences in: stimulated saliva (p=0.0019), buffer capacity (p=0.0381) and plaque pH (p=0.0430); after treatment the unstimulated saliva (p=0.0026) showed differences. Conclusions: Orthodontic treatment changes the oral environmental factors, promotes an increase in stimulated flow rate, buffer capacity and salivary pH, which augment the anti caries activity of saliva. In contrast, increased occult blood indicated more gingival inflammation, apparently because augmented the retentive plaque surfaces and the difficult to maintain a good oral hygiene, rinsed the bleeding in saliva by periodontal damage

Salival immunoglobulin gene expression in patients with caries

BACKGROUND: Immunoglobulins mediate the host’s humoral immune response are expressed in saliva. AIM: To quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition. SUBJECTS AND METHODS: This was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαμR and FcγRIIB were quantified through TaqMan Assays. RESULTS: One hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups. CONCLUSION: Although we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found

Salivary Immunoglobulin Gene Expression in Patients with Caries

BACKGROUND: Immunoglobulins mediate the host’s humoral immune response are expressed in saliva.AIM: To quantify the FcαR, FcγRIIB, and FcαμR gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition.SUBJECTS AND METHODS: This was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the FcαR, FcαµR and FcγRIIB were quantified through TaqMan Assays.RESULTS: One hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG (FcγRIIB) expression between the studied groups.CONCLUSION: Although we confirmed the existence of FcαR, FcγRIIB and FcαμR gene expression in saliva, only a significant difference in the expression of FcγRIIB between the mixed dentition and permanent dentition was found

Tratamiento quirúrgico de hiperplasia epitelial multifocal En paciente mexicana de 62 años de edad. Caso clínico

la hiperplasia epitelial focal es una enfermedad benigna de curso crónico que afecta a los niños, es producto del virus del papiloma humano y se manifiesta en la mucosa bucal, su aparición espontánea y/o permanencia en pacientes de edad avanzada, probablemente se deba a posibles factores desencadenantes el estímulo irritativo repetido de la fricción y roce de la mucosa labial, carrillos o lengua con los dientes, el hábito de mascar gomas o chicles con mínimos traumatismos acompañantes, así como el mascar tabaco, falta de vitamina K e irritación por corrientes galvánicas asociadas a amalgamas dentales, elmanejo médico de la hiperplasia epitelial focal sólo se requiere para propósitos estéticos y funcionales

Dental arch morphology of Mazahua and mestizo teenagers from central Mexico

Aim: The aim of this study was to determine the morphologic characteristics of the dental arches in the ethnic group "Mazahua" and mestizo teenagers from central Mexico. Methods: A sample of 80 Mazahua and 80 mestizo teenagers with normal occlusion, divided into two age groups, were evaluated. A digital caliper was used to measure in cast models the intercanine width, intermolar width, length and perimeter of the arch, occlusal intermaxillary curve (of Spee), overjet, and overbite. A comparative analysis with Student’s t-test was applied between gender and population groups. Results: Comparison of the dental arches between Mazahua and mestizo teenagers revealed that statistically significant differences existed with respect to most of the measurements. In most cases, they were greater in males; the Mazahua teenagers had intercanine and intermolar widths greater than mestizo teenagers. Conclusions: Each group has a characteristic dental arch form. The ethnic group Mazahua has squared arches, whereas the mestizo teenagers have oval arches, which give them their particular facial characteristics. These findings indicate that population-specific standards are necessary for clinical assessments

Dental arch morphology of Mazahua and mestizo teenagers from central Mexico

e aim of this study was to determine the morphologic characteristics of the dental arches in the ethnic group Mazahua and mestizo teenagers from central Mexico. Methods: A sample of 80 Mazahua and 80 mestizo teenagers with normal occlusion, divided into two age groups, were evaluated. A digital caliper was used to measure in cast models the intercanine width, intermolar width, length and perimeter of the arch, occlusal intermaxillary curve (of Spee), overjet, and overbite. A comparative analysis with Students t-test was applied between gender and population groups. Results: Comparison of the dental arches between Mazahua and mestizo teenagers revealed that statistically significant differences existed with respect to most of the measurements. In most cases, they were greater in males; the Mazahua teenagers had intercanine and intermolar widths greater than mestizo teenagers. Conclusions: Each group has a characteristic dental arch form. The ethnic group Mazahua has squared arches, whereas the mestizo teenagers have oval arches, which give them their particular facial characteristics. These findings indicate that population-specific standards are necessary for clinical assessments

La Ciencia de Materiales en la Facultad de Química - UAEM

El libro se encuentra conformado por 13 capítulos que son resultados parciales de tesis de maestría y doctorado, escritos por estudiantes, por su comité de tutores y, en algunos casos, por los investigadores que colaboran con cada grupo disciplinario. Por ello, está dirigido a estudiantes de posgrado en Ciencia de Materiales, y de Ingeniería en Materiales del último año de la carrera.En 1996 dieron inicio los programas de Maestría y Doctorado en Ciencia de Materiales en la Facultad de Química de la Universidad Autónoma del Estado de México. Estos programas ofrecen a los estudiantes la formación académica y de investigación, a través del desarrollo de proyectos que incluyen la síntesis, modificación y el procesamiento de nuevos materiales para aplicaciones en medicina, óptica, construcción, catálisis, detección de sustancias volátiles, entre otras. En los últimos 3 años con el apoyo de estudiantes e investigadores, se logró concluir esta obra que da a conocer la importancia de sintetizar, caracterizar y aplicar materiales con base en sus propiedades.Secretaría de Educación Pública-Subsecretaría de Educación Superior-Dirección General de Educación Superior Universitaria. Número del convenio con la SEP: 2017-15-001-017