Temporal isolation between two strongly differentiated stocks of the Greenland halibut (Reinhardtius hippoglossoides Walbaum, 1792) from the Western Barents Sea

Identification of discrete stocks of Greenland halibut is an important aspect of proper fisheries exploitation. Available literature data indicated a lack of significant inter‑area differences between Greenland halibut populations from the Northeast Atlantic. To define the population diversity, two aspects were taken into account: enzyme‑genetic diversity and the concentration of heavy metals in tissues. Seven allozyme loci variations were used to characterize the genetic structure of four populations of Greenland halibut from the Western Barents Sea region. The samples were collected from the spawning area in the period when this species took migration to spawn. The sample RH4 was significantly different from the other samples collected from the same location (RH2 and RH3) and depth for over two days. Another sample (RH8), collected from the nearby area 6 days later was similar to the samples RH2, RH3. We noticed a significant divergence between the sample RH4 and the three remaining samples, where the value of the index FST fluctuated about 0.40 and approximately 0.01 between three similar populations. This genetic fluctuation negates the thesis of a panmictic character of the Western Atlantic population. Feeding groups of Greenland halibut are moving along the Barents Sea shelf and they are exposed to different heavy metals concentrations according to the food preferences or the exact place of feeding. We identified similar concentrations of heavy metals, i.e., Zn, Cu, Cd, and Pb in all samples. Trace metal analysis of aquatic organisms from the Barents Sea can provide important information on the degree of environmental contamination, and the potential impact of seafood consumption

The influence of glycation on a high pressure denaturation of ubiquitin

Synopsis The combination of deuterium-hydrogen exchange (DHX) and mass spectrometry (MS) can be used for studying a high pressure denaturation (HPD) of proteins. Herein we present the results of investigations of the influence of glycation on the HPD of ubiquitin. Application of various values of pressure causes different degrees of protein unfolding, resulting in molecules with a different number of protons available for exchange with deuterons. The dependence of this number on pressure gives information on the denaturation state of a protein. On the basis of the obtained results we can conclude that increasing number of fructosamine moieties in ubiquitin decreases the pressure required for its denaturation. It suggests that glycation moderately decreases the protein stability. The present study is the first example of application of hydrogen-deuterium exchange as a method of investigating the influence of posttranslational modification of protein on the HPD

An Optimised Di-Boronate-ChemMatrix Affinity Chromatography to Trap Deoxyfructosylated Peptides as Biomarkers of Glycation

We report herein a novel ChemMatrix® Rink resin functionalised with two phenylboronate (PhB) moieties linked on the N-α and N-ε amino functions of a lysine residue to specifically capture deoxyfructosylated peptides, compared to differently glycosylated peptides in complex mixtures. The new PhB-Lys(PhB)-ChemMatrix® Rink resin allows for exploitation of the previously demonstrated ability of cis diols to form phenylboronic esters. The optimised capturing and cleavage procedure from the novel functionalised resin showed that only the peptides containing deoxyfructosyl-lysine moieties can be efficiently and specifically detected by HR-MS and MS/MS experiments. We also investigated the high-selective affinity to deoxyfructosylated peptides in an ad hoc mixture containing unique synthetic non-modified peptides and in the hydrolysates of human and bovine serum albumin as complex peptide mixtures. We demonstrated that the deoxyfructopyranosyl moiety on lysine residues is crucial in the capturing reaction. Therefore, the novel specifically-designed PhB-Lys(PhB)-ChemMatrix® Rink resin, which has the highest affinity to deoxyfructosylated peptides, is a candidate to quantitatively separate early glycation peptides from complex mixtures to investigate their role in diabetes complications in the clinics

PODZIAŁY DOMATYCZNE GRAFÓW I ICH PRODUKTÓW

recenzenci: prof. dr hab. Mieczysław Borowiecki, Uniwersytet Zielonogórski, Wydział Matematyki, Informatyki i Ekonometriiprof. dr hab. Adam Paweł Wojda, Akademia Górniczo-Hutnicza, Wydział Matematyki Stosowanej, Katedra Matematyki Dyskretnej

Edge decoration of anthracene switches global diatropic current that controls the acene reactivity

[Image: see text] The 14π-electron system of anthracene has been merged with the unsaturated Z-1,2-difurylethene to form a macrocycle(s) with the retained local conjugation of all incorporated subunits that were substantially modulated with a redox activation, opening a global delocalization involving all integrated aromatics. In addition, the edge modulation of acene via the attachment of a specific isomer of the conjugated system gives steric confinements that are characteristic of small macrocycles, forcing substantially short C(H)···O electrostatic interactions that are documented spectroscopically with the support of X-ray analysis

ACCEPTED MANUSCRIPT The influence of glycation on a high pressure denaturation of ubiquitin BIOSCIENCE REPORTS The influence of glycation on a high pressure denaturation of ubiquitin

The combination of deuterium-hydrogen exchange and mass spectrometry can be used for studying a high pressure denaturation of proteins. Herein we present the results of investigations of the influence of glycation on the high pressure denaturation of ubiquitin. Application of various values of pressure causes different degrees of protein unfolding, resulting in molecules with a different number of protons available for exchange with deuterons. The dependence of this number on pressure gives information on the denaturation state of a protein. On the basis of the obtained results we can conclude that increasing number of fructosamine moieties in ubiquitin decreases the pressure required for its denaturation. It suggests that glycation moderately decreases the protein stability. This study is the first example of application of hydrogen deuterium exchange as a method of investigating the influence of posttranslational modification of protein on the high-pressure denaturation

Research into leeways in the regions of the Świnoujście– Szczecin fairway on the Szczecin Lagoon

The paper analyzes the influence of air mass movement on moving (the leeway) surface water in the Świnoujście–Szczecin fairway region on the Szczecin Lagoon. The Szczecin Lagoon includes waters of the Odra River estuary (Poland’s second largest river) and the southern Baltic Sea. To calculate the leeway parameters, a relevant surface drifter was outlined and constructed. The data on the leeway of the drifter was obtained from in-situ experiments conducted on the Szczecin Lagoon in the summer of 2018. In turn, the air mass movement data was recorded at meteorological stations in Trzebież and Świnoujście. A statistical analysis of the leeway parameters of the drifter was also presented. Distributions of the leeway and wind speeds in the Świnoujście–Szczecin fairway regions were established. Moreover, linear regressions between the leeway and wind parameters were performed by decomposing the leeway into its downwind and crosswind components for each 10-minute sample. It is worth highlighting that relationships between these components of the leeway and wind parameters were studied for weak, medium, and stronger winds. This research may be useful for increasing navigation safety in the Świnoujście–Szczecin fairway regions on the Szczecin Lagoon