28 research outputs found
Revisiting AFLP fingerprinting for an unbiased assessment of genetic structure and differentiation of taurine and zebu cattle
The Drosophila melanogaster Seminal Fluid Protease “Seminase” Regulates Proteolytic and Post-Mating Reproductive Processes
Proteases and protease inhibitors have been identified in the ejaculates of animal taxa ranging from invertebrates to mammals and form a major protein class among Drosophila melanogaster seminal fluid proteins (SFPs). Other than a single protease cascade in mammals that regulates seminal clot liquefaction, no proteolytic cascades (i.e. pathways with at least two proteases acting in sequence) have been identified in seminal fluids. In Drosophila, SFPs are transferred to females during mating and, together with sperm, are necessary for the many post-mating responses elicited in females. Though several SFPs are proteolytically cleaved either during or after mating, virtually nothing is known about the proteases involved in these cleavage events or the physiological consequences of proteolytic activity in the seminal fluid on the female. Here, we present evidence that a protease cascade acts in the seminal fluid of Drosophila during and after mating. Using RNAi to knock down expression of the SFP CG10586, a predicted serine protease, we show that it acts upstream of the SFP CG11864, a predicted astacin protease, to process SFPs involved in ovulation and sperm entry into storage. We also show that knockdown of CG10586 leads to lower levels of egg laying, higher rates of sexual receptivity to subsequent males, and abnormal sperm usage patterns, processes that are independent of CG11864. The long-term phenotypes of females mated to CG10586 knockdown males are similar to those of females that fail to store sex peptide, an important elicitor of long-term post-mating responses, and indicate a role for CG10586 in regulating sex peptide. These results point to an important role for proteolysis among insect SFPs and suggest that protease cascades may be a mechanism for precise temporal regulation of multiple post-mating responses in females
Comparing the transcriptomes of embryos from domesticated and wild Atlantic salmon (Salmo salar L.) stocks and examining factors that influence heritability of gene expression
Background Due to selective breeding, domesticated and wild Atlantic salmon are genetically diverged, which raises concerns about farmed escapees having the potential to alter the genetic composition of wild populations and thereby disrupting local adaptation. Documenting transcriptional differences between wild and domesticated stocks under controlled conditions is one way to explore the consequences of domestication and selection. We compared the transcriptomes of wild and domesticated Atlantic salmon embryos, by using a custom 44k oligonucleotide microarray to identify perturbed gene pathways between the two stocks, and to document the inheritance patterns of differentially-expressed genes by examining gene expression in their reciprocal hybrids. Results Data from 24 array interrogations were analysed: four reciprocal cross types (W♀×W♂, D♀×W♂; W♀×D♂, D♀×D♂)×six biological replicates. A common set of 31,491 features on the microarrays passed quality control, of which about 62% were assigned a KEGG Orthology number. A total of 6037 distinct genes were identified for gene-set enrichment/pathway analysis. The most highly enriched functional groups that were perturbed between the two stocks were cellular signalling and immune system, ribosome and RNA transport, and focal adhesion and gap junction pathways, relating to cell communication and cell adhesion molecules. Most transcripts that were differentially expressed between the stocks were governed by additive gene interaction (33 to 42%). Maternal dominance and over-dominance were also prevalent modes of inheritance, with no convincing evidence for a stock effect. Conclusions Our data indicate that even at this relatively early developmental stage, transcriptional differences exist between the two stocks and affect pathways that are relevant to wild versus domesticated environments. Many of the identified differentially perturbed pathways are involved in organogenesis, which is expected to be an active process at the eyed egg stage. The dominant effects are more largely due to the maternal line than to the origin of the stock. This finding is particularly relevant in the context of potential introgression between farmed and wild fish, since female escapees tend to have a higher spawning success rate compared to males
A N'Dama cattle resource population for research on trypanotolerance
The genetic components of trypanotolerance measurements must be determined in well controlled on going production systems where the necessary identification and/or quantification of environment and genetic effects are possible. A resource population has been established in the Mushie District Bandundu Province, Democratic Republic of Congo, within a commercial ranch situated in one of the highest available tsetse/trypanosomosis challenge areas having cattle. Cattle are grade N'Dama selected from an original group imported into R.D. Congo 75 years ago. Breeding cows are maintained in multi-sire herds. Parentage (sire) determination in the experimental population is done through molecular genetic markers (micro-satellites). In order to evaluate the genetic diversity within the experimental population, 50 unrelated bulls were genotyped for 33 bovine microsatellites, mapping 19 chromosomes. It was concluded that the variability of and polymorphism of, the markers in the resource population is substantial and will allow accurate parentage determination. More importantly for the relevance of the research on the within-breed genetic variation of trypanotolerance measurements, it was concluded that the resource population was one of the purest N'Dama cattle population available for research, exposed to high level of natural trypanosomosis risk, as indicated by the likely absence of introgression of Zebu type genes
Genetic distance between Bos taurus breeds and a possible phylogenic tree with a Bison outgroup
Genetic distances between Bos taurus breeds and a possible phylogenetic tree with a Bison outgroup
Microsatellite variation between an African and five European taurine breeds results in a geographical phylogenetic tree with a bison outgroup.
Microsatellite variation between an African and five European taurine breeds results in a geographical phylogenetic tree with a bison outgroup
The genetic relationship between five Belgian breeds (Belgian Blue, Black Pied, Red Pied, White and Red of East Flanders, Red of West Flanders), an African taurine N'Dama breed and a captive American bison herd was studied by means of 31 microsatellite loci. On the basis of the allele frequencies of the 31 scored loci, Nei's modified genetic distance was calculated between the populations. The neighbour joining clustering algorithm was used to construct a possible phylogenetic tree. Herein American bison served as an outgrouThe dendrogram displays a geographical topology with three major lineages as expected, but is unclear in clustering the local breeds
Evaluation Of The Genetic Variability Of 23 Bovine Microsatellite Markers In Four Belgian Cattle Breeds
The polymorphism of 23 microsatellites in the four main cattle breeds in Belgium (Holstein Friesian, Belgian Blue, Belgian Red Pied and East Flemish) was analysed. Heterozygosity, polymorphism information content, the effective number of alleles, exclusion probability and the probability of genotypic identity for two random individuals were calculated for all microsatellites and all breeds. The Belgian Blue breed is generally a little less polymorphic in comparison with the other three breeds, Estimates of the genetic distances between these breeds confirmed the widely accepted proposition that the Belgian Blue is the most genetically distinct of these breeds. The three other breeds are likely to become one population, given current breeding strategies. Exclusion probabilities in parentage control cases are >0.9999 in all four breeds when all 23 microsatellites are used and >0.98 with only the two most polymorphic multiplexes
Signatures of selection? Patterns of microsatellite diversity on a chromosome containing a selected locus
This paper explores patterns of genetic diversity near a locus known to have been under selection. The myostatin gene (GDF-8) has been shown to be associated with double muscling, a phenotype selected for in a number of cattle breeds. We examined population genetic parameters for microsatellite loci at varying distances from GDF-8 in doublemuscled (DM) and non-double-muscled (non-DM) cattle breeds in order to assess patterns of diversity. A theoretical analysis was also performed to predict the patterns of diversity expected under different scenarios. We found differences in the patterns of heterozygosity, allele diversity and linkage disequilibrium between DM and non-DM breeds. However, there were some exceptions to the predicted patterns. These are discussed in light of the histories of the breeds and the potential for using microsatellite diversity for mapping trait genes in livestock populations