Anti-Schistosomal Activity of Chenopodium ambrosoides Extracts in Adult Worms In vivo and In vitro

Plants may contain ingredients that have anti-parasitic activity against parasites of medical significance. Chenopodium ambrosoides (Wormseed) a wide spread herb in the Family Chenopodiacea was investigated for anti-schistosomal activity using, the human trematode parasite, Schistosoma mansoni, as the target. The plant is well known for its vermifuge and anti-helminthetic properties. The root, stem, leaves and fruit of the plant were extracted sequentially using n-hexane, dichloromethane, methanol and distilled water as solvents and tested for anti-schistosomal activity. The crude extracts of leaves and fruits remarkable and significant activity that resulted in significant egg counts reduction, compared to untreated controls (P < 0.05). Among the plant extracts (n – hexane, dichloromethane, methanol and aqueous), aqueous (leaf) and methanol (fruit) extracts showed responses closest to PZQ. Aqueous (leaf) had 46% worms reduction, methanol (fruit) had 23% worms reduction and Praziquantel had 34% worms reduction (P > 0.05). The in vitro results showed methanol (fruit) extract killed more adult worms of S. mansoni than the aqueous (leaf) extract. Methanol (fruit) extract potency depended on concentration. The higher the concentration, the faster the killing. The effect of both methanol (fruit) and aqueous (leaf) extracts on S. mansoni adult worms showed that methanol (fruit) extract had better potency than aqueous (leaf) extract.  The killing effect of methanol (fruit) and aqueous (leaf) extracts were statistically similar to Praziquantel. Keywords: Chenopodium ambrosoides (Wormseed),  In vivo and  In vitr

Determination of Anti-schistosomal Finger Profiles of Chenopodium ambrosoide Crude Extracts in BALB/c Mice Using Thin Layer Chromatography (TLC)

Plants may contain ingredients that have anti-parasitic activity against parasites of medical siginificance. Chenopodium ambrosoides (Wormseed) a wide spread herb in the Family: Chenopodiacea was investigated for anti-schistosomal activity using, the human trematode parasite, Schistosoma mansoni, as the target. The plant is well known for its vermifuge and anti-helminthetic properties. The root, stem, leaves and fruit of the plant were extracted sequentially using n-hexane, dichloromethane, methanol and distilled water as solvents and tested for anti-schistosomal activity. TLC finger profiles mobile of C. ambrosoides extracts showed aqueous (leaf) extract had  more Rf spots than methanol (fruit) extract but they were not significantly different (P > 0.05). The results of this study suggest that Chenopodium ambrosoides aqueous (leaf) and methanol (fruit) extracts has remarkable anti-schistosomal properties, and should be investigated to determine their toxicity and also tested against other parasites as a source novel anti-parasitic compounds. Keywords: Rf - Mobility Relative to front TLC - Thin Layer Chromatograph

Anti-Schistosomal Activity of Chenopodium ambrosoides Extracts in Adult Worms In vivo and In vitro

Plants may contain ingredients that have anti-parasitic activity against parasites of medical significance. Chenopodium ambrosoides (Wormseed) a wide spread herb in the Family Chenopodiacea was investigated for anti-schistosomal activity using, the human trematode parasite, Schistosoma mansoni, as the target. The plant is well known for its vermifuge and anti-helminthetic properties. The root, stem, leaves and fruit of the plant were extracted sequentially using n-hexane, dichloromethane, methanol and distilled water as solvents and tested for anti-schistosomal activity. The crude extracts of leaves and fruits wereremarkableand showedsignificant activity that resulted in significant egg counts reduction, compared to untreated controls (P < 0.05). Among the plant extracts (n – hexane, dichloromethane, methanol and aqueous), aqueous (leaf) and methanol (fruit) extracts showed responses closest to PZQ. Aqueous (leaf) had 46% worms reduction, methanol (fruit) had 23% worms reduction and Praziquantel had 34% worms reduction (P > 0.05). The in vitro results showed methanol (fruit) extract killed more adult worms of S. mansoni than the aqueous (leaf) extract. The effect of both methanol (fruit) and aqueous (leaf) extracts on S. mansoni adult worms showed that methanol (fruit) extract had better potency than aqueous (leaf) extract.  The killing effect of methanol (fruit) and aqueous (leaf) extracts were statistically similar to Praziquantel. Keywords:Chenopodium ambrosoides (Wormseed),  In vivo and  In vitr