Evaluation of pre-clinical safety and toxicology of Althaea officinalis extracts as naturopathic medicine for common carp (Cyprinus carpio)

The current study was done to investigate the preclinical safety and toxicology of Althaea officinalis extract as naturopathic medicine in common carp (Cyprinus carpio). Specimens were treated with 0 (control), 2.5, 5 and 10 g of marshmallow extract for 45 days. Plasma biochemical parameters were measured after 15 and 45 days. Total protein, albumin and globulin levels (p0.05). During the experimental period, triglyceride levels noticeably decreased in fish fed with 2.5 g of A. officinalis extract (p<0.05). Although, administration of marshmallow extract up to 5 g per kg of feed did not show any side effect on fishes, the use of the A. officinalis extract (10 g) led to cytotoxicity and modifications in blood biochemical parameters of fish. Therefore, we recommend the use of the lower concentrations than 10 g A. officinalis extract in prospective clinical studies

Sequential sampling of Rhopalosiphum maidis and Schizaphis graminum (Hem.: Aphididae) in wheat fields of Badjgah, Fars province, Iran

Between 2010 and 2011, spatial distribution and fixed precision sequential sampling plans were determined for two aphids, Rhopalosiphum maidis F. and Schizaphis graminum R., in wheat fields in Badjgah countryside of Iranian Fars province. For this purpose, two fields of two hectares each were selected and sampled on weekly basis. Each sample consisted of 200 randomly selected wheat plants along with the total number of counted aphids. Based on RV, the best sample unit was four stems. This data was used to describe spatial distribution pattern of R. maidis and S. graminum by Taylor’s power law (TPL) and Iwao’s patchiness regression methods. The results indicated aggregated spatial distribution of aphids' populations in wheat fields, based on the mentioned methods. The TPL provided a better description of the aphids' spatial distribution. Since regression line slopes were not significantly different for S. graminum and R. maidis, the same Green model was proposed for both species. In this model, minimum numbers of samples were 20, 12 and 9 for precision levels of 0.15, 0.25 and 0.3, respectively. Comparing Green and conventional methods in 0.15, 0.25 and 0.3 precision levels showed that the number of required samples were reduced 77.8 ± 1.05, 78.3 ± 0.91 and 81.4 ± 0.81 percent, respectively. Based on Wilson and Room's model, when the mean populations of aphids were 1.6, 3.86 and 5.62, the proportion of infestation in the field were 0.5, 0.75 and 0.85, respectively. Therefore, by increasing the infestation percentage of the samples, the number of required samples will be reduced

Sequential sampling of overwintered sunn pest, Eurygaster integriceps (Het.: Scutelleridae) in rainfed wheat fields in Borujerd, Iran

The sunn pest, Eurygaster integriceps Puton, is the most important pest of wheat and barley in most wheat producing regions of Iran, including Lorestan province. The pest overwinters under bushes in mountains and returns to wheat fields in the next spring. Study of spatial distribution of overwintered sunn pest (OSP) in rainfed wheat fields using two quadrate sizes (50 à 50 cm2 = 0.25 m2 and 70.71 à 70.71 cm2 = 0.5 m2) and their conformity with negative binomial distribution, showed a dispersion pattern of aggregation. In this study, a sequential sampling plan (Waldâs method) was presented by use of 1.5 OSP/m2 as an action threshold and 1.05 OSP/m2 as the safety level, and estimating the common K (Kc) for the both sizes of quadrates in 2004 and 2005. Two sizes of quadrates were used as sample units to estimate densities and dispersion patterns of the pest populations. The decision lines, y = 0.32x ± 7.62 andy = 0.63x ± 7.44 were calculated for 0.25 and 0.5 m2 quadrates, respectively. The operating characteristic (OC) and average sample number (ASN) curves indicated that low numbers of samples are required for control decision making when the population densities are either low (less than 0.22 and 0.44 OSP/q for small and large quadrates, respectively) or high (more than 0.45 and 0.89 OSP/q for small and large quadrates, respectively). However, the number of samples required will be high when the population density is nearly medium and at the point of inflection of OC curve (0.32 and 0.63 OSP/q for small and large quadrates, respectively)

Resistance of grapevine to the erineum strain of Colomerus vitis (Acari: Eriophyidae) in western Iran and its correlation with plant features.

Trisetacus juniperinus (Nalepa) sensu Keifer (Acari: Eriophyoidea: Phytoptidae) causes irregular development of buds, shoot deformations and stunted growth of trees, resulting in a serious threat to nurseries and young stands of Cupressus sempervirens L. (Mediterranean cypress). Recently, some cypress clones selected for their resistance to the fungal canker agent Seiridium cardinale (Wag.) have shown high susceptibility to the mite. Considering its tiny body, its hidden lifestyle inside the buds and the probable occurrence of other species (the vagrant Epitrimerus cupressi (Keifer) is common on the Mediterranean cypress in Italy), detection and monitoring of T. juniperinus require taxonomic expertise and are often time-consuming and challenging before serious damage is discernible. In the present study, a rapid, cost-effective PCR-based method was developed and validated to detect T. juniperinus on cypresses. The cytochrome c oxidase subunit I gene was amplified with degenerate and specific primers, but the latter were the only ones able to discriminate between T. juniperinus and E. cupressi. PCR products distinguished the two species both in a pool of individuals in a mixed population of both species and in single individuals, indicating the sensitivity of the detection method. PCR–RFLP (restriction fragment length polymorphism) by means of XmnI and XbaI endonucleases separated the two species. Furthermore, a washing-sieving protocol was used to make mite collection from the tree sample faster and simpler; this procedure did not interfere with the molecular detection of the species. The possibility of the routine use of this assay to monitor quarantine eriophyoids infesting plant material is discussed