Evaluation of Lipase-Pancreatin Administration on Testicular Structure Followed by L-arginine-Induced Pancreatitis in Mice

Background & Objective: L-arginine-induced experimental pancreatitis was evaluated as a distinct and new form of experimental pancreatitis with particular consideration to the therapeutic role of lipase-pancreatin and its effects on testicular tissue structure. Materials & Methods: Fifty mice were classified into: 1) control; 2) induced pancreatitis (IP) by administration of L-arginine solution (300 mg/100g); 3) IP by L-arginine solution (300 mg/100g) along with lipase- pancreatin; 4) IP group with L-arginine solution (400 mg/100g); 5) IP group by L-arginine solution (400 mg/100g) along with lipase- pancreatin. L-arginine solution was administered only once at the beginning of the course by intraperitoneal injection and lipase-pancreatin treatments were twice daily for 14 consecutive days and started 72 hours later after L-arginine administration. Finally, tissues samples were prepared for stereological analysis by SPSS 23 software and one-way analysis of variance (One-way ANOVA). Results: The results showed that after experimental pancreatitis with different doses of L-arginine, the mean area and diameter of seminiferous tubules and the area of spermatogonia cells in the treated groups were significantly decreased or increased in comparison with the control group, However, no significant difference was observed in the area of spermatid cells and primary spermatocytes between the treatment and control groups. It was also observed that in the lipase-pancreatin-treated groups, the tissue changes caused by L-arginine-induced pancreatitis were significantly compensated. Conclusion: It can be concluded that the L-arginine-induced pancreatitis has caused testicular tissue changes and the use of lipase-pancreatin solution has been shown to be partly effective in reducing these changes