Processing on recognition of FTIR-MSP alteration of Heart tissue during mice fetal life

Introduction: Understanding of fetus development is one of the most complicated with a great impact on Teratology. FTIR-MSP is among the most useful spectroscopy technique for biological and cellular application. In this project, various statistical calculations were used for recognition and discrimination of heart tissue spectra during 9.5-17.5 days of mice fetal life. Method and Results: The mice fetuses were dissected on day 9.5-17.5 of gestation and then fixed by fixative solution. Tissue sections (10 µm) were used for FTIR-MSP measurement in the wavenumber region of 4000-400 cm-1. Spectra were preceded by baseline correction, smoothing, deconvolution and 2nd derivatisation. PCA, ANN and SVM have been used to find the most relevant modifications in during fetus development. PCA with adjusting data mass and seven selected major PCs have been used to find the most relevant modifications in different steps of mice fetus heart tissue development and also BP-FF ANN and SVM classifications could diagnose different steps of development up to 96.3% and 92.59% respectively. Conclusions: PCA, ANN and SVM methods could classify and discriminate the FTIR spectroscopic data and can be as a new potential tool for the teratogenic investigations

The effects of radiofrequency radiation on mice fetus weight, length and tissues

The public concern of harmful effects of radiofrequency radiation exposure, especially with rapid increase in the use of wireless and telecommunication devices, is increasing. Some studies show fetal and developmental abnormalities as the result of radiofrequency radiation exposure. We aimed to investigate possible teratogenic effects of radiofrequency in 915 MHz on mice fetus and protective role of vitamin C. 21 pregnant mice were divided into 3 groups. Control group was in normal condition without any stressor agent. Exposure group was exposed to 915 MHz RFR (8 h/day for 10 days) and 0.045 µw/cm2 power density. The exposure plus vitamin C group received 200 mg/kg vitamin C by gavage and was exposed to 915 MHz RFR (8 h/day for 10 days) and 0.045 µw/cm2 power density. The fetus weight, C-R length were measured by digital balance and caliper. Tissues were assessed after staining with H & E. Our results showed significant increase in fetus weight and C-R length and also enlarged liver, tail deformation in mice fetus in exposure group. Although usage of vitamin C caused significant decrease in mentioned parameters. The outcome of this study confirms the effects of radiofrequency radiation on growth parameters such as body weight, length and some tissues in mice fetuses and protective effect of vitamin C. However more studies on non-ionization radiation in different frequencies and severity, during pregnancy are needed to clarify the exact mechanisms of these changes and better protection. Keywords: Radiofrequency radiation, Mice fetus tissues, Vitamin C, Fetal and developmental abnormalitie