9 research outputs found
Proizvodnja goveÄeg mesa obogaÄenog organski vezanim selenom
In this study, the effects of the organic and inorganic form of selenium, in the diet for finishing beef cattle, were investigated. Sell-Plex produced by American firm Alltech as a source of organic selenium with selenium concentration of 2000 mg/kg was used. The source of inorganic selenium was sodium selenite. The trial was conducted on Simmental fattening cattle in the final stage in the free housing system and it lasted 60 days. Selenium content in control and trial group (m. longissimus dorsi) was 78.65 and 125.03 Ī¼g/kg (P (lt) 0.01), in kidneys 855.52 and 1026.04 Ī¼g/kg(P (lt) 0.001); in liver 453.13 and 534.64 Ī¼g/ kg(P (lt) 0.01) and in spleen 308.44 and 383.62 Ī¼g/kg (P (lt) 0.001), respectively. There were no differences in main carcass characteristics, share of some tissues in the round and three rib chops, chemical composition and meat quality of m. longissimus dorsi (P>0.05).Considering the higher storage of organic selenium in meat and internal organs of the fattening cattle with respect to the inorganic form, it was concluded that the selenium supply in organic form has a better bioavailability. Inclusion of organic selenium into the diet in the final stage of fattening cattle and its deposition in meat and internal organs is a good and easy way to get meat with functional foods properties that in addition to nutritional value should positively affect human health.U radu su ispitani efekti organskog i neorganskog selena u obrocima junadi u tovu. Kao ozvor organskog selena koriÅ”Äen je preparat Sell-Plex u kome je koncentracija selena iznosila 2000 mg/kg a izvor neorganskog selena bio je natrijumselenit. Ogled je izveden na Simentalskoj rasi goveda u zavrÅ”noj fazi tova u slobodnom sistemu držanja i trajao je 60 dana. Sadržaj selena u m. longissimus dorsi, jetri, slezini i bubrezima bio je znaÄajno viÅ”i na ishranbenom tretmanu sa organskim selenom u odnosu na neorganski, Å”to ukazuje na njegovu bolju bioiskoristivost. UraÄena je disekcija polutke prema zvaniÄno važeÄoj skali za klasifikaciju trupova odraslih goveda propisanoj od strane evropske zajednice. Dobijeni rezultati ukazuju da nije bilo razlike izmeÄu tretmana sa organskim i neorganskim selenom u telesnoj masi grla pre klanja kao i glavnim karakteristikama polutke. TakoÄe, nije bilo razlike u udelu pojedinih tkiva u butu i trorebarnom iseÄku kao i ni u hemijskom sastavu i kvalitetu mesa u m. longissimus dorsi. ImajuÄi u vidu da je selen esencijalan mikroelement u ishrani a da organski vezan selen ima bolju bioiskoristivost u odnosu na neorganski, njegovo ukljuÄivanje u obroke goveda i deponovanje u mesu i unutraÅ”njim organima je dobar i jednostavan naÄin za dobijanje mesa sa svojstvima funkcionalne hrane koja treba da pored nutritivne vrednosti pozitivno utiÄe na zdravlje ljudi. PoÅ”to su pojedini delovi planete Zemlje slabo opskrbljeni sa selenom ovo je najjednostavniji naÄin da se reÅ”i problem njegovog deficita
V struÄno-nauÄni skup sa meÄunarodnim uÄeÅ”Äem "Aktuelnosti u edukaciji i rehabilitacija osoba sa smetnjama u razvoju"
Efekat dodavanja selena u hranu na kvalitet mesa
The health of farm animals depends on many factors and they all indicate that food plays an important role in preserving health, improving reproductive and productive characteristics and functioning of immune system. Besides numerous nutritional factors, antioxidants play a special role in the struggle for survival and good health state. An oxidative stress represents a condition induced by generating and reacting of reactive-oxygen species (ROS), and their toxic products, on different metabolic and physiological processes. The generating of ROS is induced by both endogenous and exogenous factors. In the case of the organism inability to expel the causative agents of oxidative stress they can damage cell lipids, proteins or DNA thus endangering the cell functions. Lipid peroxidation is a process which can lead to degradation of lipids and damage of cell membrane. Selenoenzyme glutathione peroxidase can protect organism from peroxidative damages, decrease the level of malondialdehyde protecting in this way a muscular tissue from oxidation. Recent research has shown that selenium has an effect of preserving sensory characteristics of meat and its texture, what is of great importance for consumers. Animal tissue incorporates supplemented selenium quickly thus it is possible to produce a selenium enriched meat and eggs. Selenium also shows positive effects on meat quality: it reduces water loss, influence the stability of colour, prolongs oxidative protection, and therefore the time of its shelf life.Zdravlje životinja zavisi od brojnih faktora, a sve ukazuje da hrana ima važnu ulogu u oÄuvanju zdravlja, poboljÅ”anju reproduktivnih i proizvodnih karakteristika i funkcionisanju imunskog sistema. Pored brojnih hranidbenih faktora, antioksidansi imaju posebnu ulogu u borbi za opstanak i dobro zdravstveno stanje. Oksidativni stres je stanje izazvano stvaranjem i reagovanjem reaktiv-oksigen vrsta (ROS) i njihovih toksiÄnih produkata na razliÄite metaboliÄke i fizioloÅ”ke procese. Stvaranje ROS izazivaju endogeni i egzogeni faktori. Ukoliko organizam nije u stanju da otkloni uzroÄnike oksidativnog stresa oni mogu da oÅ”tete Äelijske lipide, proteine ili DNA, a time da ugroze funkcije Äelija. Lipidna peroksidacija je proces koji dovodi do degradacije lipida i oÅ”teÄenja Äelijske membrane. Selenoenzim glutation peroksidaza Å”titi organizam od peroksidativnih oÅ”teÄenja, smanjuje nivo malondialdehida i na taj naÄin Å”titi miÅ”iÄna tkiva od oksidacije. IzuÄavanja poslednjih godina ukazuju da selen utiÄe na oÄuvanje senzornih karakteristika mesa i njegove teksture, Å”to je od velikog znaÄaja za konzumente. Životinjsko tkivo brzo inkorporira dodati selen i tako je moguÄe proizvesti selenom obogaÄeno meso i jaja. Selen ispoljava pozitivne efekte i na kvalitet mesa: redukuje gubitak teÄnosti, utiÄe na postojanost boje, produžava oksidativnu zaÅ”titu, a time i vreme njegove upotrebe
In vitro culture as a tool for improvement of cabbage cultivars in Yugoslavia
Cabbage (Brassica oleracea var. capitata) is one of the most common vegetables in Yugoslavia. Our breeding programs on Brassicas are developed with the aim to create genetically pure lines which could be used for creation of various hybrids and new cultivars. Since there are barriers which prevent fast realization of these goals, we have introduced in vitro culture techniques in our breeding programs. Thus we investigated possibilities of using micropropagation, anther culture, in vitro selection and genetic engineering methods as tools to improve our Brassicas and create new perspective genotypes suitable for uses in conventional breeding methods. So far we have clonned a number of perspective Brassica genotypes and identified the most appropriate medium for their growth and multiplication. We present here our efforts to overcome incompatibility barriers by attempting to create double-haploid genotypes of cabbage through anther culture. Identifying possible tolerance of our domestic populations to herbicide by in vitro selection is also one of our goals. We have also created our first transformed Brassicas, performing Agrobacterium-mediated transformation. In this paper we have presented our research in these areas
Results of the project 'Workshops for improvement the practical skills of people with intellectual disabilities'
IV International Scientific Conference Special Education and Rehabilitation - Early Interventio
Samozastupanje osoba sa intelektualnom ometenoÅ”Äu ā teorijski okvir
IV struÄno-nauÄni skup sa meÄunarodnim uÄeÅ”Äem Aktuelnosti u edukaciji i rehabilitaciji osoba sa smetnjama u razvoju
Razvoj veÅ”tina samozastupanja osoba sa intelektualnom ometenoÅ”Äu ā preliminarno saopÅ”tenje
IV struÄno-nauÄni skup sa meÄunarodnim uÄeÅ”Äem Aktuelnosti u edukaciji i rehabilitaciji osoba sa smetnjama u razvoj
Transformations of Brassicaceae family
KupusnjaÄe (Brassicaceae) su ekonomski veoma znaÄajna familija biljaka. U poslednjih trideset godina jako mnogo se radi na poboljÅ”avanju karakteristika vrsta roda Brassica. Medu novijim metodama koje se koriste u oplemenjivaÄke svrhe na kupusima jesu i tehnike genetiÄkog inženjerstva. Cilj ovog rada bio je da razmotri transformacije kupusnjaÄa, prikaže preliminarne rezultate dobijem u naÅ”oj laboratoriji, gde je izvrÅ”ena inokulacija nekoliko genotipova roda Brassica. KoriÅ”Äenje soj A. tumefaciens koji nosi bar gen za rezistenciju na herbicid Basta. kao i gen za rezistenciju na kanamicin (nptII) i reporter gen za-glukuronidazu (GUS). U radu je dat i osvrt na svrsishodnost transformacija Brassicacea, kao i na eventualne rizike koje koriÅ”Äenje transgenih kupusa nosi.Brassicaceae are economically very important crop family. Improvement of its characteristics is in focus for the last thirty years very much. One of the methods of biotechnology used in Brassica genus is genetic transforĀmation. In this paper we have discussed transformations of Brassica genus, comparing it with the results that we achieved in our laboratory. We have performed inoculation with Agrobacterium tumefacien, with the construct containing resistance to herbicide Basta (bar), marker genes for Kanamycin resistance (npt II) and reporter gene-glucuronidase. We have also discussed risks of transgenic crop use and seed development