The hemp seeds were treated with diethyl sulphate, in four concentrations (0.1%, 0.25%, 0.5%, and 1%) and in 
two variants of alkylant exposure (3 and 6 hours). The length growth of plantlets, mitotic index and frequency of 
chromosomal aberrations were the analyzed parameters. Significant modifications were obtained at the level of 
parameters in variants treated with DES, comparatively with control