Perfluorocarbon restrains inflammation and cell apoptosis in rats with lung ischemia reperfusion injury via down regulation of TLR4/NF κB signaling pathway

Purpose: To exaluate the effect of perfluorocarbon on lung ischemia-reperfusion injury in rats, and to unravel the potential underlying mechanism.Methods: A total of 36 Sprague-Dawley (SD) rats were randomly assigned to sham group, model group, and perfluorocarbon group (12 rats per group). The levels of inflammatory factors (TNF-α and IL1β) were determined using enzyme-linked immunosorbent assay (ELISA). Cell apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, while Western blotting was conducted to determine the protein expressions of TLR4 and NF-κB.Results: The levels of inflammatory factors in the model and perfluorocarbon groups were significantly higher than those in operation group (p < 0.05), while their levels in perfluorocarbon group were significantly lower than in model group (p < 0.05). The mRNA expression levels of TNF-α and IL-1β in lung tissues rose significantly in both model and perfluorocarbon groups when compared with those in sham group (p < 0.05), but declined significantly in the perfluorocarbon group in comparison with those in model group (p < 0.05). Furthermore, the perfluorocarbon group exhibited a significantly lower cell apoptosis than model group (p < 0.05). The relative protein expression levels of TLR4 and NF-κBdeclined significantly in perfluorocarbon group than in model group.Conclusions: Perfluorocarbon down-regulates the TLR4/NF-κB signaling pathway, and inhibits inflammation a

Distinguish bipolar and major depressive disorder in adolescents based on multimodal neuroimaging:Results from the Adolescent Brain Cognitive Development study^®

Background: Major depressive disorder and bipolar disorder in adolescents are prevalent and are associated with cognitive impairment, executive dysfunction, and increased mortality. Early intervention in the initial stages of major depressive disorder and bipolar disorder can significantly improve personal health. Methods: We collected 309 samples from the Adolescent Brain Cognitive Development study, including 116 adolescents with bipolar disorder, 64 adolescents with major depressive disorder, and 129 healthy adolescents, and employed a support vector machine to develop classification models for identification. We developed a multimodal model, which combined functional connectivity of resting-state functional magnetic resonance imaging and four anatomical measures of structural magnetic resonance imaging (cortical thickness, area, volume, and sulcal depth). We measured the performances of both multimodal and single modality classifiers. Results: The multimodal classifiers showed outstanding performance compared with all five single modalities, and they are 100% for major depressive disorder versus healthy controls, 100% for bipolar disorder versus healthy control, 98.5% (95% CI: 95.4–100%) for major depressive disorder versus bipolar disorder, 100% for major depressive disorder versus depressed bipolar disorder and the leave-one-site-out analysis results are 77.4%, 63.3%, 79.4%, and 81.7%, separately. Conclusions: The study shows that multimodal classifiers show high classification performances. Moreover, cuneus may be a potential biomarker to differentiate major depressive disorder, bipolar disorder, and healthy adolescents. Overall, this study can form multimodal diagnostic prediction workflows for clinically feasible to make more precise diagnose at the early stage and potentially reduce loss of personal pain and public society

<i>Neisseria</i> species as pathobionts in bronchiectasis

Neisseria species are frequently identified in the bronchiectasis microbiome, but they are regarded as respiratory commensals. Using a combination of human cohorts, next-generation sequencing, systems biology, and animal models, we show that bronchiectasis bacteriomes defined by the presence of Neisseria spp. associate with poor clinical outcomes, including exacerbations. Neisseria subflava cultivated from bronchiectasis patients promotes the loss of epithelial integrity and inflammation in primary epithelial cells. In vivo animal models of Neisseria subflava infection and metabolipidome analysis highlight immunoinflammatory functional gene clusters and provide evidence for pulmonary inflammation. The murine metabolipidomic data were validated with human Neisseria-dominant bronchiectasis samples and compared with disease in which Pseudomonas-, an established bronchiectasis pathogen, is dominant. Metagenomic surveillance of Neisseria across various respiratory disorders reveals broader importance, and the assessment of the home environment in bronchiectasis implies potential environmental sources of exposure. Thus, we identify Neisseria species as pathobionts in bronchiectasis, allowing for improved risk stratification in this high-risk group.Published versio

Design and Parameter Optimization of Fruit&ndash;Soil Separation Device of Lily Harvester

The mechanized harvesting of lily fruit in Southern China is affected by a high damage rate of lily fruit and low rate of soil breakage. The existing fruit&ndash;soil separation device is not suitable for heavy soil in Southern China. This study aimed to design a flexible fruit&ndash;soil separation device that can effectively reduce the damage rate of lily and improve the crushing rate of the soil. Thus, it would meet the requirements of southern lily fruit harvesting. In this study, the soil breaking rate and lily damage rate in the fruit&ndash;soil separation were taken as the assessment indexes, and the linear speed of the front conveyor belt, the inclination angle and the rotating speed of the fruit&ndash;soil separation device were recognized as the test factors. By performing the Box&ndash;Behnken test, three-factor and three-level experimental research on the fruit&ndash;soil separation device of the lily harvester was conducted. On this basis, a multiple regression model of the assessment indexes to the respective was built, the effect of various factors on the operation quality was analyzed and optimal operation parameters were determined. When the optimal parameter was adopted in the test (e.g., the linear speed of the front conveyor belt, the inclination angle and the speed of the fruit&ndash;soil separation device reaching 1.2 m&middot;s&minus;1, 36&deg; and 98 r&middot;min&minus;1, respectively), the soil crushing rate was 92.8% and the lily damage rate reached 8.9%, and the fruit&ndash;soil separation effect satisfied the requirements of lily fruit harvest. The results could be referenced for other subsoil fruit harvesters and fruit&ndash;soil separation devices under heavy clay soil in Southern China

Design and Parameter Optimization of Fruit–Soil Separation Device of Lily Harvester

The mechanized harvesting of lily fruit in Southern China is affected by a high damage rate of lily fruit and low rate of soil breakage. The existing fruit–soil separation device is not suitable for heavy soil in Southern China. This study aimed to design a flexible fruit–soil separation device that can effectively reduce the damage rate of lily and improve the crushing rate of the soil. Thus, it would meet the requirements of southern lily fruit harvesting. In this study, the soil breaking rate and lily damage rate in the fruit–soil separation were taken as the assessment indexes, and the linear speed of the front conveyor belt, the inclination angle and the rotating speed of the fruit–soil separation device were recognized as the test factors. By performing the Box–Behnken test, three-factor and three-level experimental research on the fruit–soil separation device of the lily harvester was conducted. On this basis, a multiple regression model of the assessment indexes to the respective was built, the effect of various factors on the operation quality was analyzed and optimal operation parameters were determined. When the optimal parameter was adopted in the test (e.g., the linear speed of the front conveyor belt, the inclination angle and the speed of the fruit–soil separation device reaching 1.2 m·s−1, 36° and 98 r·min−1, respectively), the soil crushing rate was 92.8% and the lily damage rate reached 8.9%, and the fruit–soil separation effect satisfied the requirements of lily fruit harvest. The results could be referenced for other subsoil fruit harvesters and fruit–soil separation devices under heavy clay soil in Southern China

Thermal Conductivity of Silica-aerogel (SA) and Autoclave Aerated Concrete (AAC) Composites

Improving the thermal insulating performance of porous building materials is of great practical significance for building energy conservation. In this work, silica aerogels (SA) with ultralow thermal conductivity were proposed as an appropriate candidate to be integrated with autoclaved aerated concrete (AAC) to produce novel SA-AAC composites with higher thermal insulating performance by physical solution impregnation method. The pore-structures, mechanical and thermal properties of the SA-AAC composites were probed by various experimental tests. According to the microscopy and porosimetry results, SA were observed to adhere to the surface walls of the AAC holes, thus reducing the amount of macro-sized pores. In addition, the improved thermal insulating performance of AAC was successfully achieved with the relative improvement depending on the porosity of the pristine AAC. At the mass fraction of SA of ~7%, the highest relative improvement was found to be ~30% The results of this work exhibited a great potential of this novel SA-AAC composite in engineering applications

Lithium Enrichment by the Carbothermal Reduction of Spodumene Ore and the Preparation of Manganese-Silicon Alloy

To increase the low utilization rate of spodumene ore during lithium extraction, spodumene ore was subjected to carbothermic reduction to enrich lithium and prepare a manganese-silicon alloy. The experimental results showed that during thermal reduction, lithium was volatilized and collected in the condensation zone. The Li2O content in the lithium condensate was 41.72%, which was 10.85 times higher than that of the raw material. The effects of varying reduction temperatures and times on the lithium volatilization rate and direct yield of Mn5Si3 alloy were investigated. The best process conditions were 1873 K for 6 h. Under these conditions, the lithium volatilization rate was 97.65%, and the direct yield of Mn5Si3 was 86.47%

Study on Recovery of Lithium from Lithium-Containing Aluminum Electrolyte

The current process of recovering lithium from wasted aluminum electrolyte mostly entails extracting lithium from lithium-containing aluminum electrolyte by acid leaching and dissipation. Aiming at the disadvantages of the existing treatment method, such as the long process flow, environmental pollution, poor working environment, etc., we propose a new technology to extract lithium from the wasted aluminum electrolyte and systematically investigate the effects of raw material particle size, holding time, temperature and other factors on the recovery of lithium. The results show that the better process conditions for the recovery of lithium are as follows: the raw material particle size is 75~150 μm, the additive is CaCl2, the mass ratio of calcium chloride to lithium-containing aluminum electrolyte is 3:5, the reaction temperature is 1473 K, and the holding time is 3 h. After the product of the reaction is crushed and leaching is carried out by using deionized water (pH = 6.8), the temperature of the leaching is 368 K, the leaching time is 3 h, and the solid–liquid ratio is 1/3, and the leaching rate of Li can be up to 75.1%. In addition, the purity of the recovered AlF3 is more than 92.7%. This process realizes the comprehensive and efficient use of lithium-containing aluminum electrolyte and provides a new idea for the development of lithium extraction technology from lithium-containing aluminum electrolyte

Lithium Enrichment by the Carbothermal Reduction of Spodumene Ore and the Preparation of Manganese-Silicon Alloy

To increase the low utilization rate of spodumene ore during lithium extraction, spodumene ore was subjected to carbothermic reduction to enrich lithium and prepare a manganese-silicon alloy. The experimental results showed that during thermal reduction, lithium was volatilized and collected in the condensation zone. The Li2O content in the lithium condensate was 41.72%, which was 10.85 times higher than that of the raw material. The effects of varying reduction temperatures and times on the lithium volatilization rate and direct yield of Mn5Si3 alloy were investigated. The best process conditions were 1873 K for 6 h. Under these conditions, the lithium volatilization rate was 97.65%, and the direct yield of Mn5Si3 was 86.47%