Analyse de la stabilité au feu des murs en béton armé par l'approche calcul à la rupture

High rise reinforced concrete walls under fire exhibit important out-of-plane displacements, which in turn lead to an eccentricity of the self-weight with respect to the initial undeformed configuration, resulting in supplementary bending moments. This geometrical change, combined with the degradation of the stiffness and strength properties of reinforced concrete due to severe temperature increase, may lead to the failure of walls under fire.Investigation on fire resistance of reinforced concrete walls will be based on the yield design approach in order to analyze the global stability of high rise walls, taking into account the geometry changes induced by the thermal loading.The program consists of two parts.Firstly, a 9-meter high reinforced concrete wall has already been tested under fire with the equipment Vulcan. This full size experiment aims at validating the modeling of plates under large out-of-plan displacement and identifying potential local phenomenon which has not been considered in the model.Secondly, a yield design approach will carried out to analyze the stability of reinforced concrete walls. By using the perturbation method, a recursive analytical procedure based on a kinematic approach is proposed to find the deformed configuration of reinforced concrete walls under fire. The deformed configuration will be later modeled as a shallow shell, on which a yield design procedure will be performed by a non-linear optimizationPour les panneaux de grande hauteur soumis à un fort gradient thermique associé à l’incendie, ils subissent des déplacements hors plan importants qui, du fait de l’excentrement du poids propre qui en résulte, vont engendrer des efforts de flexion venant s’ajouter aux efforts de compression déjà existants. Un tel changement de géométrie, d’autant plus prononcé que le panneau est de grande hauteur, combiné à une dégradation simultanée des propriétés de résistance des matériaux sous l’effet de l’élévation de température, peut conduire à un effondrement de la structure sous poids propre. L’évaluation de la résistance au feu d’éléments de structures de grandes dimensions, repose sur la théorie du calcul à la rupture, appliquée d’une part à la détermination d’un diagramme d’interaction au feu caractérisant la résistance du panneau en chacune de ses sections, d’autre part à l’analyse de la ruine globale du panneau dans sa configuration déformée. Le programme comporte deux volets complémentaires. Le premier volet concerne l’approche expérimentale. Un mur de 9 mètre a été testé sur le grand équipement Vulcain afin de valider le modèle de calcul d’une part, qui est en cours du développement, et à identifier des phénomènes locaux éventuels non accessibles par la modélisation d’autre part. Le deuxième volet concerne le développement d’outils de modélisation et de calcul performants. Basées sur une approche cinématique, des procédures itératives qui se reposent sur la méthode de perturbation ont été construit pour chercher une solution analytique pour prédire la configuration déformée d’une plaque soumis à un fort gradient thermique. Ensuite, des méthodes numériques fondées sur la discrétisation en éléments finis du panneau déformé, traité comme une coque à faible courbure, et l’utilisation de techniques d’optimisation non-linéaires, qui ont connu récemment des progrès importants, devront pouvoir être développées et appliquées à ce problèm

Stability of reinforced concrete walls under fire conditions by a yield design approach

Pour les panneaux de grande hauteur soumis à un fort gradient thermique associé à l’incendie, ils subissent des déplacements hors plan importants qui, du fait de l’excentrement du poids propre qui en résulte, vont engendrer des efforts de flexion venant s’ajouter aux efforts de compression déjà existants. Un tel changement de géométrie, d’autant plus prononcé que le panneau est de grande hauteur, combiné à une dégradation simultanée des propriétés de résistance des matériaux sous l’effet de l’élévation de température, peut conduire à un effondrement de la structure sous poids propre. L’évaluation de la résistance au feu d’éléments de structures de grandes dimensions, repose sur la théorie du calcul à la rupture, appliquée d’une part à la détermination d’un diagramme d’interaction au feu caractérisant la résistance du panneau en chacune de ses sections, d’autre part à l’analyse de la ruine globale du panneau dans sa configuration déformée. Le programme comporte deux volets complémentaires. Le premier volet concerne l’approche expérimentale. Un mur de 9 mètre a été testé sur le grand équipement Vulcain afin de valider le modèle de calcul d’une part, qui est en cours du développement, et à identifier des phénomènes locaux éventuels non accessibles par la modélisation d’autre part. Le deuxième volet concerne le développement d’outils de modélisation et de calcul performants. Basées sur une approche cinématique, des procédures itératives qui se reposent sur la méthode de perturbation ont été construit pour chercher une solution analytique pour prédire la configuration déformée d’une plaque soumis à un fort gradient thermique. Ensuite, des méthodes numériques fondées sur la discrétisation en éléments finis du panneau déformé, traité comme une coque à faible courbure, et l’utilisation de techniques d’optimisation non-linéaires, qui ont connu récemment des progrès importants, devront pouvoir être développées et appliquées à ce problèmeHigh rise reinforced concrete walls under fire exhibit important out-of-plane displacements, which in turn lead to an eccentricity of the self-weight with respect to the initial undeformed configuration, resulting in supplementary bending moments. This geometrical change, combined with the degradation of the stiffness and strength properties of reinforced concrete due to severe temperature increase, may lead to the failure of walls under fire.Investigation on fire resistance of reinforced concrete walls will be based on the yield design approach in order to analyze the global stability of high rise walls, taking into account the geometry changes induced by the thermal loading.The program consists of two parts.Firstly, a 9-meter high reinforced concrete wall has already been tested under fire with the equipment Vulcan. This full size experiment aims at validating the modeling of plates under large out-of-plan displacement and identifying potential local phenomenon which has not been considered in the model.Secondly, a yield design approach will carried out to analyze the stability of reinforced concrete walls. By using the perturbation method, a recursive analytical procedure based on a kinematic approach is proposed to find the deformed configuration of reinforced concrete walls under fire. The deformed configuration will be later modeled as a shallow shell, on which a yield design procedure will be performed by a non-linear optimizatio

Evaluating the failure load of high-rise reinforced concrete walls under fire loading using the yield design approach

International audienceThe present contribution is addressing the investigation on the failure of high-rise reinforced concrete walls subject to fire loading. In Yang (2018), a 2D plate model has been developed for predicting the deflections and subsequent geometry changes of tall reinforced concrete panels under severe fire exposure. Such a deformed geometry of the wall is a key ingredient to its stability analysis by means of the yield design theory, which is the subject of the present paper. The practical implementation of the approach is based on shell finite elements and a generalized strength criterion accounting for reduced strength capacities of the constitutive materials. For illustrative purposes, numerical simulations based on typical values of input data (geometrical as well as material parameters), are conducted to investigate the sensitivity of the wall stability to the temperature increase on the one hand, and to its geometrical parameters and boundary conditions, on the other hand. One of the main conclusions which can be drawn from the present analysis is that the boundary conditions prescribed along the vertical lateral sides of the wall have a decisive influence on its stability

The Effect of Blue Light on the Production of Citrinin in Monascus purpureus M9 by Regulating the mraox Gene through lncRNA AOANCR

Blue light, as an important environmental factor, can regulate the production of various secondary metabolites of Monascus purpureus M9, including mycotoxin-citrinin, pigments, and monacolin K. The analysis of citrinin in Monascus M9 exposed to blue light for 0 min./d, 15 min./d, and 60 min./d showed that 15 min./d of blue light illumination could significantly increase citrinin production, while 60 min./d of blue light illumination decreased citrinin production. Analysis of long non-coding RNA (LncRNA) was performed on the transcripts of Monascus M9 under three culture conditions, and this analysis identified an lncRNA named AOANCR that can negatively regulate the mraox gene. Fermentation studies suggested that alternate respiratory pathways could be among the pathways that are involved in the regulation of the synthesis of citrinin by environmental factors. Aminophylline and citric acid were added to the culture medium to simulate the process of generating cyclic adenosine monophosphate (cAMP) in cells under illumination conditions. The results of the fermentation showed that aminophylline and citric acid could increase the expression of the mraox gene, decrease the expression of lncRNA AOANCR, and reduce the yield of citrinin. This result also indicates a reverse regulation relationship between lncRNA AOANCR and the mraox gene. A blue light signal might regulate the mraox gene at least partially through lncRNA AOANCR, thereby regulating citrinin production. Citrinin has severe nephrotoxicity in mammals, and it is important to control the residual amout of citrinin in red yeast products during fermentation. LncRNA AOANCR and mraox can potentially be used as new targets for the control of citrinin production

Full-scale fire test on a high-rise RC wall

International audienceThis paper describes a full-scale test carried out on a high-rise reinforced concrete wall subjected to fire loading conditions in the special Vulcain furnace (very large and modular gas furnace) at CSTB, France. The test sample was designed to represent a slender wall with a very large height/thickness ratio. The experimental results showed a large deflection of the high wall in fire conditions (almost equal to the wall height/24) even for a fire exposure time of 90 min. Such a deflection was significantly higher than the maximum value recommended by design codes (about the wall height/30 for 120 min of fire exposure). Furthermore, other test results (full-field displacements in the 3 directions, residual deformed shape after the heating and cooling phases) may also provide additional useful experimental data for validating available or future models (which are usually based on some specific assumptions). In addition, the measurements of thermal-induced deformed shape of the wall are compared to those predicted by an analytical solution derived from a simplified one-dimensional (1D) beam model, as well as to those obtained from non-linear finite-element simulations (with 2D plate elements), in order to assess the practical applicability of these simulations. These comparisons show a reasonably good agreement between the experimental results and the predictions of these models, thereby providing a first validation of the latter

Transcriptomic Insights into Benzenamine Effects on the Development, Aflatoxin Biosynthesis, and Virulence of <i>Aspergillus flavus</i>

Aspergillus flavus is a soilborne pathogenic fungus that poses a serious public health threat due to it contamination of food with carcinogenic aflatoxins. Our previous studies have demonstrated that benzenamine displayed strong inhibitory effects on the mycelial growth of A. flavus. In this study, we systematically investigated the inhibitory effects of benzenamine on the development, aflatoxin biosynthesis, and virulence in A. flavus, as well as the underlying mechanism. The results indicated that benzenamine exhibited great capacity to combat A. flavus at a concentration of 100 &#181;L/L, leading to significantly decreased aflatoxin accumulation and colonization capacity in maize. The transcriptional profile revealed that 3589 genes show altered mRNA levels in the A. flavus after treatment with benzenamine, including 1890 down-regulated and 1699 up-regulated genes. Most of the differentially expressed genes participated in the biosynthesis and metabolism of amino acid, purine metabolism, and protein processing in endoplasmic reticulum. Additionally, the results brought us to a suggestion that benzenamine affects the development, aflatoxin biosynthesis, and pathogenicity of A. flavus via down-regulating related genes by depressing the expression of the global regulatory factor leaA. Overall, this study indicates that benzenamine have tremendous potential to act as a fumigant against pathogenic A. flavus. Furthermore, this work offers valuable information regarding the underlying antifungal mechanism of benzenamine against A. flavus at the level of transcription, and these potential targets may be conducive in developing new strategies for preventing aflatoxin contamination

Table_1_Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities.DOC

To understand the mechanisms of immunomodulatory effect, Dendrobium Officinale polysaccharides (DOP) were treated by ultrasound and mild base separately to generate fractions of various weight-average molecular weight (Mw) and degrees of acetylation (DA). The structural features, conformational properties, functional properties and immunomodulatory activities of original and modified DOPs were investigated. Ultrasonic treatment decreased the Mw and apparent viscosity and improved the water solubility of DOP. Mild base treatment remarkably reduced the DA and the water solubility, while the overall apparent viscosity was increased. Conformational analysis by triple-detector high performance size-exclusion chromatography showed that the molecular chain of DOP turned more compact coil conformation with decreased DA. Results from the macrophages RAW 264.7 analysis showed that samples sonicated for 200 min (Mw 34.2 kDa) showed the highest immune-regulation effects. However, the immunomodulatory effects of the samples after de-acetylation were all compromised compared to the original DOP. This study inspires further research to establish the structural-immunomodulatory relationships, which promote the application of DOP in both the food and medicine fields.</p